Hujing HBS-1101 Microplate Reader

Overview

The Hujing HBS-1101 Microplate Reader is a compact, benchtop absorbance-based microplate detection system engineered for precision and reliability in routine and research-grade life science laboratories. Operating on the principle of photometric absorbance measurement—where light attenuation through sample-containing wells is quantified according to the Beer-Lambert law—the instrument delivers consistent optical performance across the visible to near-infrared spectrum (400–1000 nm). Its vertically oriented 8-channel optical architecture minimizes cross-talk and ensures high well-to-well reproducibility, while the thermally stabilized halogen light source and precision filter wheel enable accurate, low-drift measurements essential for ELISA, protein assays, cell viability studies, and enzymatic kinetics. Designed to meet the operational expectations of quality-controlled environments, the HBS-1101 supports traceable calibration workflows and complies with fundamental metrological requirements validated by third-party verification reports.

Key Features

• 8-channel vertical optical path design for simultaneous multi-well acquisition and minimized optical crosstalk
• High-stability 12 V DC halogen lamp with integrated power management to extend lamp lifetime and reduce thermal drift
• Expandable filter capacity supporting up to 15 interchangeable interference filters—standard set includes 405, 450, 492, and 630 nm for common immunoassay applications
• 0.001 OD resolution with ≤ ±0.01 OD accuracy and ≤ ±0.003 OD baseline stability over 30 minutes at 450 nm
• Programmable plate shaking function with three amplitude levels and precise timer control (0–255 s)
• 7-inch capacitive touchscreen interface enabling intuitive graphical plate layout, real-time data preview, and on-device protocol configuration
• Robust mechanical architecture housed in a compact footprint (400 × 260 × 200 mm), optimized for space-constrained lab benches

Sample Compatibility & Compliance

The HBS-1101 is compatible with standard 96-well microplates—including flat-bottom, U-bottom, and V-bottom formats—as well as opaque or clear polystyrene, polypropylene, and quartz plates. It supports both single- and dual-wavelength measurements, accommodating background subtraction and spectral correction protocols required for turbid or colored matrices. While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 out-of-the-box, its software includes audit trail logging, user access levels, and electronic signature-ready report generation—features aligned with GLP and internal GMP-aligned quality systems. The device carries a formal metrological verification certificate issued by an accredited Chinese calibration laboratory, confirming conformance to JJG 861–2015 (Chinese national verification regulation for microplate readers). All firmware and software components are covered under registered copyright protection (China Copyright Protection Center No. 2022SR0XXXXXX), ensuring intellectual property integrity and long-term support continuity.

Software & Data Management

The bundled PC-based analysis software provides a comprehensive, modular workflow environment for assay development, execution, and reporting. Core functionalities include endpoint, two-point, kinetic, and dual-wavelength measurement modes; nonlinear curve fitting using four-parameter logistic (4PL) and five-parameter logistic (5PL) algorithms; dynamic threshold-based result classification; and statistical quality control evaluation via Levey-Jennings charts and Westgard multirule analysis. Data export options include CSV, Excel (.xlsx), PDF, and XML formats, with full support for LIMS integration via standardized file I/O. The software maintains a time-stamped, immutable audit trail for all critical actions—including plate mapping edits, parameter changes, and result approvals—satisfying documentation requirements for internal QA/QC audits and regulatory readiness assessments.

Applications

The HBS-1101 serves as a foundational platform for quantitative colorimetric assays in academic, clinical, and industrial settings. Typical use cases include ELISA quantification of cytokines and antibodies, Bradford/Lowry/BCA protein assays, MTT/XTT/CCK-8 cell proliferation and cytotoxicity screening, β-galactosidase and alkaline phosphatase reporter gene assays, and kinetic monitoring of enzyme activity (e.g., LDH, ACE, or proteases). Its broad wavelength range also permits adaptation to non-standard assays requiring custom filter sets—such as NAD(P)H oxidation at 340 nm or heme-based peroxidase reactions at 570 nm—when configured with optional filters.

FAQ

Is the HBS-1101 compliant with FDA 21 CFR Part 11?
The instrument and software are not pre-certified to 21 CFR Part 11; however, the audit trail, electronic signature support, and role-based access controls provide a configurable foundation for Part 11 compliance within validated internal environments.
Can the HBS-1101 perform fluorescence or luminescence measurements?
No—this is a dedicated absorbance reader. It does not include excitation sources, emission filters, or PMT detectors required for fluorescence or luminescence detection.
What filter wavelengths are available beyond the standard set?
Optional filters spanning 340–1000 nm are available upon request, including 340, 540, 570, 595, 620, and 850 nm, subject to mechanical compatibility and calibration validation.
Does the software support automated plate barcode scanning?
Barcode reading is not natively supported; however, external USB barcode scanners can be integrated via Windows HID drivers for manual ID entry into sample metadata fields.
How is calibration verified and maintained?
Users may perform daily optical verification using NIST-traceable neutral density filters or certified reference standards; full recalibration services are offered by authorized Hujing service centers under the 3-year warranty program.