INNOTEG EasyPrep HP High-Pressure Preparative Liquid Chromatography System

Overview

The INNOTEG EasyPrep HP is a high-pressure preparative liquid chromatography (PLC) system engineered for reproducible, scalable purification of synthetic intermediates, natural product isolates, and pharmaceutical compounds. It operates on the principle of high-resolution liquid-phase separation under elevated backpressure—typically up to 3000 psi—enabling efficient resolution of structurally similar analytes using both reversed-phase (C18, C8) and normal-phase (silica, amino) columns with internal diameters ranging from 10 mm to 50 mm. Unlike analytical HPLC systems, the EasyPrep HP is purpose-built for milligram-to-gram scale isolation with integrated fraction collection, real-time UV monitoring, and binary gradient solvent delivery. Its architecture supports method translation from analytical to preparative conditions, facilitating seamless workflow integration in discovery chemistry, process development, and quality control laboratories.

Key Features

• High-precision dual-piston reciprocating pump delivering stable flow rates from 0.1 to 100 mL/min with ≤0.5% RSD across the full range.
• Binary gradient capability with independent solvent selection and programmable mixing ratios (0–100% in 0.1% increments), supporting method robustness and solvent economy.
• UV-Vis detector with selectable wavelength ranges (200–400 nm standard; optional 200–800 nm configuration for broader chromophore detection including conjugated aromatics and transition metal complexes).
• Intelligent fraction collector with automatic tube rack recognition, enabling precise spatial indexing and traceable sample handling.
• Real-time operational flexibility: users may modify gradient profiles via drag-and-drop interface, adjust flow rate during active runs, and insert manual collection triggers without interrupting separation.
• Integrated hardware safety protocols—including continuous leak sensing, pressure transducer-based over-pressure cutoff, and temperature-monitored solvent reservoirs—ensure compliance with ISO 13849-1 functional safety guidelines for laboratory equipment.

Sample Compatibility & Compliance

The EasyPrep HP accommodates diverse sample matrices encountered in drug discovery and natural product research, including crude reaction mixtures, plant extracts, fermentation broths, and peptide digests. Its stainless-steel fluidic path (316L SS pump heads, valves, and tubing) resists corrosion from acidic, basic, and organic mobile phases (e.g., TFA/H₂O, TEA/CHCl₃, MeOH/H₂O). The system meets mechanical and electrical safety requirements per IEC 61010-1 and is designed to support Good Laboratory Practice (GLP) and Good Manufacturing Practice (GMP) workflows. Optional software modules provide electronic signature functionality, user access control tiers, and immutable audit trails aligned with FDA 21 CFR Part 11 Annex 11 expectations. All column hardware interfaces conform to ISO 8536-4 standards for high-pressure fittings.

Software & Data Management

The proprietary EasyChrom Control Suite offers a Windows-based graphical interface optimized for preparative method development and execution. It supports method import/export in ASTM E1946-compliant format, enables batch processing of multiple methods with auto-scheduling, and logs all instrument parameters—including pressure traces, UV chromatograms, fraction metadata, and operator actions—with time-stamped digital signatures. Raw data files are stored in vendor-neutral HDF5 format, ensuring long-term readability and third-party analysis compatibility (e.g., with MATLAB, Python pandas, or commercial cheminformatics platforms). Network deployment options allow centralized instrument management across multi-user facilities while maintaining local data sovereignty.

Applications

The EasyPrep HP serves as a core purification platform in academic, industrial, and contract research settings. Typical use cases include: isolation of chiral intermediates in asymmetric synthesis; purification of oligonucleotides and modified peptides post-solid-phase synthesis; fractionation of alkaloid-rich botanical extracts for structure–activity relationship studies; recovery of monoclonal antibody fragments following enzymatic cleavage; and preparative-scale separation of regioisomers in heterocyclic medicinal chemistry. Its pressure and flow capabilities make it especially suitable for flash chromatography replacement, orthogonal purification after SFC, and early-stage API purification where throughput and method fidelity are prioritized over ultra-high resolution.

FAQ

What column dimensions are supported?
The system is validated for preparative columns with internal diameters from 10 mm to 50 mm and lengths up to 250 mm, accommodating both packed and pre-packed cartridges.
Is method transfer from analytical HPLC possible?
Yes—scaling rules based on constant linear velocity and retention time normalization are embedded in the software’s method translation assistant, minimizing re-optimization effort.
Does the system support regulatory-compliant data archiving?
With optional validation packages, the software supports 21 CFR Part 11-compliant electronic records, including role-based permissions, change history tracking, and PDF/A-2 export with embedded digital signatures.
Can I integrate third-party detectors or fraction collectors?
The system provides TTL and RS-232/485 interfaces for external device synchronization; however, native functionality (e.g., peak-triggered collection) is fully realized only with INNOTEG-certified accessories.
What maintenance intervals are recommended?
Pump seal replacement every 6 months under continuous operation; UV lamp calibration quarterly; system suitability testing prior to each regulated batch run per USP guidance.