Jiapeng HD-2000 UV Detector
| Brand | Jiapeng |
|---|---|
| Origin | Shanghai, China |
| Model | HD-2000 |
| Detection Wavelengths | 220 nm, 254 nm, 280 nm, 340 nm |
| Flow Cell | 100 µL volume, 3 mm path length |
| Absorbance Range | 0.05 A to 2 A (7 ranges) |
| Wavelength Accuracy | ±1 nm |
| Detector Type | Fixed-wavelength UV photometer |
| Gradient Capability | Linear gradient support |
| Interface | GE/AKTA-compatible column fittings |
| Display | LED-based, triple-channel OD readout (simultaneous display of three absorbance values) |
| Control | Embedded microcontroller architecture |
Overview
The Jiapeng HD-2000 UV Detector is a benchtop fixed-wavelength photometric detector engineered for real-time monitoring of biomolecular elution profiles during liquid chromatography purification workflows. It operates on the principle of ultraviolet absorbance spectroscopy, leveraging Beer–Lambert law-based quantification at four discrete, user-selectable wavelengths—220 nm (peptide bond), 254 nm (nucleic acid backbone), 280 nm (aromatic amino acids), and 340 nm (background reference or solvent correction). Designed for integration into low-to-medium pressure chromatographic systems—including ion exchange (IEX), gel filtration, and hydrophobic interaction chromatography—the HD-2000 delivers stable baseline performance and high signal reproducibility under continuous flow conditions. Its 3 mm path-length, 100 µL flow cell enables reliable detection of dilute protein and nucleic acid samples without requiring pre-concentration, supporting typical flow rates from 0.5 mL/h up to 14.5 mL/h when paired with compatible peristaltic or syringe pumps.
Key Features
- Four fixed-wavelength LED light sources (220 nm, 254 nm, 280 nm, 340 nm) with uniform spectral output and rapid thermal stabilization
- Triple-channel digital LED display showing simultaneous absorbance (A) values at three selected wavelengths—enabling real-time ratio analysis (e.g., A260/A280) without external software
- Seven-step absorbance range selection (0.05 A to 2 A) optimized for dynamic response across low-abundance and high-concentration eluates
- Flow cell with 3 mm optical path and 100 µL internal volume—designed for minimal dispersion and high recovery in analytical- to preparative-scale separations
- Microcontroller-based embedded system with non-volatile parameter storage and zero-drift calibration routine
- Standard Luer-lock and GE/AKTA-compatible column interface ports for seamless integration into existing purification platforms
- Low baseline drift ( 0.999 over full range) verified per ISO 17025 traceable protocols
Sample Compatibility & Compliance
The HD-2000 is routinely deployed in GMP-adjacent research environments for process development of therapeutic proteins, monoclonal antibodies, plasmid DNA, and oligonucleotides. Its wavelength set aligns with USP , EP 2.2.25, and ASTM E275–21 guidelines for UV-Vis detection in biopurification. While not certified for regulated manufacturing use out-of-the-box, the instrument supports audit-ready operation when integrated with compliant data acquisition systems (e.g., validated LabChart or custom Python-based logging tools adhering to FDA 21 CFR Part 11 requirements for electronic records). The detector is compatible with aqueous and mild organic mobile phases (≤30% acetonitrile, ≤20% methanol), and its quartz-free optical path eliminates UV absorption artifacts common in fused-silica alternatives below 220 nm.
Software & Data Management
The HD-2000 operates as a standalone analog/digital hybrid detector: absorbance signals are output via 0–1 V DC analog voltage (BNC) and TTL-level digital trigger pulses synchronized to fraction collection events. Raw signal acquisition requires externally connected chart recorders, DAQ systems (e.g., National Instruments USB-6009), or PC-based oscilloscope software. For GLP-compliant workflows, users commonly pair the detector with open-source tools such as Phyton-based ChromaLog or commercial packages like Uniscan Chromatography Suite—both capable of timestamped peak integration, baseline correction, and export to CSV/PDF formats. Audit trails, user access logs, and electronic signatures must be implemented at the host software level, as the HD-2000 itself does not embed Part 11 functionality.
Applications
- Real-time monitoring of IEX elution gradients during antibody capture and polishing steps
- Quantitative tracking of His-tagged protein elution in IMAC purification
- Routine assessment of nucleic acid purity (A260/A280, A260/A230) directly from column effluent
- Method development for polysaccharide or glycoprotein separation using dual-wavelength subtraction
- Educational use in undergraduate biochemistry labs for teaching chromatographic theory and detector fundamentals
- Preparative-scale fraction screening in natural product isolation workflows
FAQ
Is the HD-2000 suitable for HPLC applications?
No—it is designed for low-pressure FPLC and gravity-fed or peristaltic-pump-driven systems (max. operating pressure < 2 bar). It lacks the flow cell design and pressure rating required for analytical or UHPLC use.
Can the flow cell be replaced with a micro-volume variant?
Yes—Jiapeng offers optional 20 µL and 50 µL flow cells with identical 3 mm path length; these require recalibration and are sold separately.
Does the detector support wavelength scanning?
No—HD-2000 is a fixed-wavelength instrument. It does not contain a monochromator or diode array; spectral flexibility is achieved by manual LED selection only.
What is the minimum detectable concentration for bovine serum albumin (BSA)?
At 280 nm and 1 A full scale, the theoretical LOD is ~48 µg/mL for standard flow conditions (1 mL/min, 3 mm path), consistent with published extinction coefficient (ε280 = 43,824 M⁻¹cm⁻¹).
Is firmware upgrade capability available?
Firmware is stored in masked ROM and not field-upgradable; functional enhancements are delivered via hardware revision (e.g., HD-2000 v2.1 introduces improved temperature compensation).
