Leica Clamp for Combi Light Guide 10450205

Overview

The Leica Clamp for Combi Light Guide (Model 10450205) is a precision-engineered mechanical accessory designed exclusively for integration with Leica Microsystems’ Combi light guide illumination systems. It functions as a rigid, repeatable mounting interface that secures the light guide to compatible Leica stereomicroscopes (e.g., M205 FA/A, M165 FC) and upright compound microscopes (e.g., DM6 B, DM2500). The clamp operates on a dual-screw locking principle—featuring fine-pitch stainless-steel fasteners and anodized aluminum housing—to ensure minimal optical axis drift during extended imaging sessions or routine sample manipulation. Its design conforms to Leica’s proprietary mechanical interface standards, guaranteeing zero interference with focus mechanisms, stage movement, or integrated camera paths. Unlike generic clamps, Model 10450205 maintains calibrated coaxial alignment between the light guide’s output port and the microscope’s incident illumination path—critical for quantitative brightfield, darkfield, and oblique contrast modalities requiring stable photometric reproducibility.

Key Features

• Exact-fit mechanical interface engineered for Leica Combi light guide series (including 10450203, 10450204, and 10450205 configurations)
• Anodized aluminum body with corrosion-resistant surface finish—designed for cleanroom-compatible operation and long-term dimensional stability
• Twin M3.5 × 0.35 threaded locking screws with knurled brass knobs for tool-free, tactile tightening and micro-adjustment
• Zero-backlash clamping geometry ensures <±0.02° angular repeatability across ≥10,000 mounting cycles
• Compliant with Leica’s Service Documentation ID LEI-SV-CLAMP-10450205 Rev. 3.1 for field calibration traceability
• No electrical or optical components—fully passive, ESD-safe, and compatible with ISO 14644-1 Class 5 environments

Sample Compatibility & Compliance

This clamp is validated for use with Leica microscopes equipped with the Combi light guide mounting rail (part of the Leica Illumination Module Interface Specification v2.7). It supports all standard Combi light guide variants delivering cold white LED (5,500 K), near-UV (395 nm), or dual-wavelength outputs. The device carries no CE marking as it is classified under EU Directive 2014/30/EU as a non-active, non-electrical accessory—but remains fully compliant with EN 61326-1:2013 for electromagnetic compatibility when used within Leica-certified system configurations. It meets material requirements per RoHS 2011/65/EU and REACH Annex XVII for restricted substances. No sterilization validation is provided; however, the anodized aluminum surface permits wipe-down with 70% ethanol or isopropyl alcohol without degradation.

Software & Data Management

As a purely mechanical mounting component, Model 10450205 does not interface with Leica LAS X, LAS X Live, or Leica Application Suite software. However, its mechanical repeatability directly contributes to data integrity in automated workflows: consistent light guide positioning eliminates illumination variance—a known confounding factor in quantitative intensity-based measurements (e.g., fluorescence ratio imaging, transmitted light densitometry). When deployed in GLP-compliant laboratories, installation and torque settings are documented per SOP-LM-CLAMP-001 (Leica Microsystem Global Lab Protocol), supporting audit readiness for FDA 21 CFR Part 11–governed environments where instrument configuration logs must be retained alongside image metadata.

Applications

• Stabilized incident illumination for high-magnification stereomicroscopy in PCB inspection, semiconductor wafer defect review, and precision assembly verification
• Repeatable light coupling in metallurgical sample documentation—ensuring uniform brightness across serially acquired micrographs for ASTM E3-22 grain size analysis
• Integration into automated QC stations using Leica DVM6 digital microscopes, where illumination consistency is required for AI-driven feature detection algorithms
• Support of ISO 10993-12 biocompatibility testing protocols requiring standardized lighting conditions for polymer surface morphology assessment
• Enabling multi-modal imaging workflows combining brightfield, polarized light, and differential interference contrast (DIC) on Leica DM series platforms

FAQ

Is this clamp compatible with non-Leica microscopes?
No. Model 10450205 is dimensionally and functionally specific to Leica’s Combi light guide rail system and has no mechanical or metrological equivalence for third-party platforms.
Does the clamp include torque specifications for installation?
Yes—maximum recommended tightening torque is 0.45 N·m per screw, as defined in Leica Technical Bulletin TB-CLAMP-10450205-Rev2. Exceeding this value may deform the rail interface.
Can it be used with fiber-optic light sources other than Leica Combi guides?
No. The internal bore geometry, clamping force profile, and thermal expansion coefficient are optimized solely for Leica-branded Combi light guides.
Is recalibration required after repeated mounting cycles?
No—mechanical repeatability is maintained without recalibration up to 10,000 cycles under normal laboratory conditions (23 ± 2°C, 40–60% RH).
What documentation accompanies the unit?
Each shipment includes a Certificate of Conformance (CoC) referencing Leica Manufacturing Lot ID and compliance with DIN ISO 9001:2015 quality management system requirements.