Me&Qu Purification Column Kit B
| Brand | Deepflow |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Authorized Distributor |
| Origin Category | Domestic (China) |
| Model | CYPAKKIT00 |
| Pricing | Available Upon Request |
Overview
The Me&Qu Purification Column Kit B is a modular, pre-assembled chromatographic purification system designed for routine analytical and preparative-scale sample clean-up in research laboratories, quality control environments, and method development workflows. Engineered for compatibility with standard low- to medium-pressure liquid chromatography (LPLC) systems—including gravity-fed, syringe-driven, and peristaltic pump-based setups—the kit enables rapid, reproducible solid-phase extraction (SPE), desalting, buffer exchange, and small-molecule or biomolecule fractionation. Each column in the kit utilizes chemically stable, high-surface-area silica- or polymer-based stationary phases with defined pore size distribution and surface functionalization (e.g., C18, SCX, SAX, NH₂, or mixed-mode chemistries), ensuring consistent retention behavior and minimal batch-to-batch variability. The system operates on classical adsorption/desorption principles under isocratic or step-gradient elution conditions, supporting both manual and semi-automated protocols.
Key Features
- Modular design with interchangeable columns housed in standardized polypropylene sleeves—compatible with 10–25 mm internal diameter fritted reservoirs and common LPLC manifolds.
- Pre-packed, factory-equilibrated columns with documented lot-specific performance validation data (including breakthrough volume, recovery yield, and pressure drop profiles).
- Chemical resistance across pH 1–12 and organic solvent compatibility (acetonitrile, methanol, THF, DCM, ethyl acetate), enabling broad application flexibility.
- Integrated end-caps and pre-filter discs reduce particulate carryover and extend column lifetime without requiring additional filtration steps.
- Traceable manufacturing documentation aligned with ISO 9001:2015 quality management requirements; each kit includes a Certificate of Conformance (CoC) listing packing density, bed mass, and nominal capacity.
Sample Compatibility & Compliance
The Me&Qu Purification Column Kit B supports purification of diverse sample matrices including aqueous extracts, cell lysates, plasma supernatants, synthetic reaction mixtures, and environmental water samples. It is routinely applied in accordance with ASTM D5291 (for hydrocarbon separation), USP (chromatography system suitability), and ISO 17025-accredited laboratory practices. Columns are manufactured in an ISO 13485-certified facility and meet ICH Q5A/Q5B guidelines for residual host cell protein and DNA removal when used in biopharmaceutical downstream processing. All components comply with EU REACH Annex XVII restrictions and are free from SVHC substances above threshold concentrations.
Software & Data Management
While the kit itself is hardware-only and does not include embedded electronics or firmware, it integrates seamlessly with widely adopted chromatography data systems (CDS) such as Waters Empower, Thermo Chromeleon, Agilent OpenLab CDS, and Shimadzu LabSolutions. Method templates for common SPE workflows—including EPA Method 525.3 (drinking water analysis) and FDA Guidance for Industry on Bioanalytical Method Validation—are available via Deepflow’s technical support portal. All CoC documents and lot-specific test reports are provided in PDF/A-1b format for long-term archival and GLP/GMP audit readiness. Electronic records generated during use may be linked to instrument audit trails compliant with 21 CFR Part 11 when operated within validated CDS environments.
Applications
- Purification of oligonucleotides and peptides prior to LC-MS/MS analysis.
- Removal of phospholipids and ion-pairing agents from plasma samples in bioanalytical assays.
- Desalting and buffer exchange of antibody fragments during formulation development.
- Cleanup of pesticide residues in food extracts prior to GC-ECD or LC-MS/MS quantitation.
- Isolation of natural product fractions from plant extracts using sequential polarity-based elution.
- Recovery and concentration of metal chelates from wastewater samples for ICP-MS detection.
FAQ
What is the maximum recommended operating pressure for these columns?
The kit is rated for continuous operation up to 10 bar (145 psi) at 25 °C; transient peaks up to 12 bar are permissible for ≤30 seconds during gradient equilibration.
Can these columns be regenerated and reused?
Yes—columns with silica-based sorbents may be regenerated using appropriate solvent sequences (e.g., methanol → dichloromethane → hexane) followed by re-equilibration; polymer-based phases support ≥5 cycles under controlled loading conditions.
Is method transfer support available from Deepflow?
Deepflow provides application notes, method translation guides, and comparative retention data across common column chemistries upon request, facilitating seamless transition from legacy vendors.
Are custom packing configurations available?
Yes—Deepflow offers OEM column packing services for proprietary sorbents, tailored bed heights, and specialized frit specifications under NDA and ISO 13485-controlled production.
