PerkinElmer Lambda 4A UV-Vis Spectrophotometer (Refurbished)

Overview

The PerkinElmer Lambda 4A UV-Vis Spectrophotometer is a double-beam, scanning spectrophotometer engineered for high-precision quantitative and qualitative analysis across the ultraviolet and visible spectral range (190–1100 nm). Based on monochromator-driven dispersive optics with a high-stability tungsten-halogen and deuterium lamp source, the Lambda 4A delivers robust photometric accuracy, low stray light (<0.01% at 220 nm), and excellent baseline stability—critical for routine QC testing, method validation, and research-grade absorbance measurements. This refurbished unit has undergone full functional verification at SpectraLab Scientific’s ISO/IEC 17025-aligned calibration facility, including wavelength accuracy verification (±0.3 nm), photometric linearity assessment (0–3.0 A), and baseline repeatability testing under controlled environmental conditions.

Key Features

• Double-beam optical design with real-time reference compensation for enhanced drift resistance and measurement reproducibility
• Scanning speed up to 2400 nm/min with programmable scan rate and data interval resolution (down to 0.1 nm)
• Standard 1 nm spectral bandwidth, optimized for resolution/sensitivity balance in pharmaceutical, environmental, and academic applications
• Integrated microprocessor control with front-panel keypad and LCD display; compatible with legacy PerkinElmer WinUV software via RS-232 interface
• Robust mechanical architecture featuring precision-ground optical mounts, temperature-stabilized detector housing, and vibration-damped baseplate
• Refurbishment includes full optical alignment, lamp replacement (if aged beyond 75% of rated life), cuvette holder recalibration, and comprehensive electrical safety testing

Sample Compatibility & Compliance

The Lambda 4A supports standard 10 mm pathlength quartz, glass, or plastic cuvettes (including matched pairs for ratio-based measurements), as well as optional accessories such as solid-sample holders, fiber-optic probes, and Peltier-controlled temperature cells. All refurbished units comply with IEC 61010-1 safety standards and meet ASTM E275–22 specifications for spectrophotometer performance verification. While not supplied with 21 CFR Part 11-compliant software out-of-box, the instrument’s hardware architecture is fully compatible with validated third-party data acquisition platforms used in GLP and GMP environments. Documentation includes a Certificate of Refurbishment, traceable wavelength and photometric verification reports, and a full service history log.

Software & Data Management

This Lambda 4A operates natively with PerkinElmer’s WinUV v4.x software suite (Windows XP/Vista/7 compatible), enabling spectral acquisition, kinetic profiling, multi-component quantitation, and derivative spectroscopy. Raw absorbance/transmittance data are exportable in ASCII, CSV, and JCAMP-DX formats for integration into LIMS or statistical analysis tools (e.g., JMP, MATLAB, R). For regulated laboratories, SpectraLab provides optional post-refurbishment software validation packages—including IQ/OQ documentation templates, electronic audit trail configuration guidance, and instrument-specific risk assessments aligned with ISO 14971. All firmware versions are verified against PerkinElmer’s official release notes to ensure compatibility and security patch compliance.

Applications

The Lambda 4A serves as a foundational analytical tool across diverse sectors: in pharmaceutical QC labs for assay validation and excipient interference screening per USP ; in environmental testing for nitrate, phosphate, and COD quantification following APHA Standard Methods; in academic research for enzyme kinetics (e.g., NADH oxidation), nanoparticle size estimation via surface plasmon resonance, and polymer degradation studies; and in food & beverage quality control for color index determination (e.g., AOAC 993.01) and preservative concentration monitoring. Its proven reliability over decades of field use—particularly in resource-constrained or high-throughput settings—makes it a cost-optimized alternative to newer platform instruments without compromising metrological integrity.

FAQ

Is this instrument eligible for extended warranty or service contracts?

Yes—SpectraLab offers optional 12- and 24-month extended service agreements covering parts, labor, and preventive maintenance visits.
Does the refurbishment include optical recalibration traceable to NIST standards?

Yes—wavelength and photometric calibration are performed using NIST-traceable holmium oxide and neutral density filters, with full documentation provided.
Can this Lambda 4A be integrated into an automated sample handling system?

It supports standard autosampler interfaces (e.g., PerkinElmer AS-90) via TTL trigger and analog voltage output; compatibility with third-party robotic handlers requires custom cabling and signal mapping verification.
What consumables and spare parts are available for long-term support?

SpectraLab maintains inventory of deuterium and tungsten-halogen lamps, fused silica cuvettes, desiccant cartridges, fuses, and optical alignment tools—all with documented shelf-life and lot traceability.
Is remote diagnostic support available after purchase?

Yes—secure remote access (via TeamViewer with customer authorization) is included in all service plans for firmware troubleshooting, parameter optimization, and operator training sessions.