PerkinElmer Lambda Bio UV-Vis Spectrophotometer (Refurbished)
| Brand | PerkinElmer |
|---|---|
| Origin | USA |
| Model | Lambda Bio |
| Instrument Type | UV-Visible Spectrophotometer |
| Age | 4 years |
| Warranty | 6 months |
| Configuration | Complete system with standard accessories |
| Compliance | Meets original factory specifications for optical performance and photometric accuracy |
Overview
The PerkinElmer Lambda Bio UV-Vis Spectrophotometer is a compact, high-stability double-beam spectrophotometer engineered for routine quantitative and qualitative analysis in academic, industrial, and quality control laboratories. Based on a Czerny-Turner monochromator design with a holographic grating and silicon photodiode detector, the instrument delivers reliable absorbance, transmittance, and reflectance measurements across the 190–1100 nm spectral range. Its double-beam architecture compensates for source drift and environmental fluctuations in real time, ensuring high reproducibility (< ±0.002 A at 0.5 A, typical) and baseline stability (< 0.0005 A/h). Designed for robust daily operation, the Lambda Bio features a thermally stabilized optical bench and precision-machined mechanical components to maintain wavelength accuracy (±0.5 nm) and photometric linearity (±0.005 A from 0.0 to 2.0 A) over extended use—attributes validated during full functional refurbishment.
Key Features
- Double-beam optical path with automatic reference beam correction for enhanced stability and reduced measurement drift
- Wavelength scanning range: 190–1100 nm with 1.0 nm nominal bandwidth (fixed slit)
- Photometric range: –0.3 to 3.0 A (Absorbance), 0–300% T (Transmittance), –99.9 to +99.9% R (Reflectance)
- Integrated cuvette holder accommodating 10 mm pathlength cells; compatible with optional holders for microvolume, flow-through, or solid-sample accessories
- Onboard memory for up to 100 methods and 500 spectra; USB port for data export and firmware updates
- Refurbished and fully calibrated per original PerkinElmer Lambda Bio specification; all optical surfaces cleaned, alignment verified, and lamp intensity optimized
- Includes deuterium and tungsten-halogen lamps, power supply, RS-232 interface cable, and user documentation set
Sample Compatibility & Compliance
The Lambda Bio supports standard quartz, glass, and plastic cuvettes (10 mm pathlength), as well as optional accessories for specialized applications—including fiber-optic probes, integrating spheres for diffuse reflectance, and temperature-controlled cell holders. It complies with ISO 9001–certified refurbishment protocols and meets the optical performance criteria defined in ASTM E275 (Standard Practices for Describing and Measuring Performance of UV-Vis Spectrophotometers) and ISO/IEC 17025–aligned calibration traceability. While not pre-configured for 21 CFR Part 11 compliance, the instrument’s data export capability enables integration into validated laboratory information management systems (LIMS) supporting GLP/GMP workflows when paired with appropriate audit-trail software.
Software & Data Management
The Lambda Bio operates independently via its front-panel interface or connects to Windows-based PCs using PerkinElmer’s legacy WinLab™ software (v3.x or earlier), which provides full scan, kinetics, multi-wavelength, and concentration analysis modes. All refurbished units include licensed software installation media and activation support. Raw spectral data are exportable in ASCII (.txt) or CSV format for post-processing in third-party tools (e.g., OriginLab, MATLAB, Python pandas). Internal memory retains method parameters and last-scan spectra even after power cycling—ensuring continuity in high-throughput environments where PC dependency must be minimized.
Applications
This instrument is routinely deployed for nucleic acid and protein quantification (A260/A280), enzyme kinetics assays (e.g., NADH oxidation at 340 nm), colorimetric endpoint assays (e.g., Bradford, Lowry, BCA), dissolution testing in pharmaceutical QC, water quality monitoring (nitrate, phosphate, COD), and thin-film thickness estimation via interference fringe analysis. Its stability and ease of use make it particularly suitable for teaching labs, contract testing facilities, and manufacturing sites requiring cost-effective, low-maintenance UV-Vis instrumentation with documented metrological integrity.
FAQ
Is this instrument covered by a manufacturer warranty?
No—this is a refurbished unit sold with a 6-month limited warranty provided by the authorized refurbisher, covering defects in materials and workmanship under normal laboratory use.
Does the system include a computer or software license?
The instrument includes WinLab™ software media and a valid single-user license; however, a compatible Windows PC (Windows 7–10, 32-bit) must be supplied separately.
Has the spectrophotometer been recalibrated before shipment?
Yes—each unit undergoes full optical recalibration using NIST-traceable standards for wavelength accuracy (Hg and D₂ emission lines) and photometric accuracy (neutral density filters), with full calibration report included.
Can I upgrade to a newer PerkinElmer software platform?
WinLab™ is the final supported software version for the Lambda Bio platform; no official migration path exists to newer PerkinElmer platforms such as UV WinControl or Spectrum Touch.
What maintenance is recommended after installation?
Annual lamp replacement (deuterium and tungsten-halogen), quarterly cleaning of the sample compartment and optical windows with spectroscopic-grade methanol, and biannual verification of wavelength and photometric accuracy using certified reference materials.
