PreFluid WF600G-YZ35 High-Precision Dispensing Peristaltic Pump

Overview

The PreFluid WF600G-YZ35 is a high-precision dispensing peristaltic pump engineered for repeatable, traceable, and scalable liquid handling in regulated laboratory and pilot-scale production environments. Unlike basic peristaltic pumps relying on open-loop speed control, the WF600G-YZ35 integrates closed-loop servo motor control with real-time feedback to maintain consistent rotational velocity across variable load conditions — critical for volumetric accuracy in low- to medium-viscosity fluid dispensing. Its core operating principle relies on occlusion-based positive displacement: rollers compress a flexible tubing segment against a rigid housing, creating discrete fluid parcels whose volume correlates directly with motor rotation count, tubing inner diameter, and compression geometry. This mechanism ensures inherent fluid isolation, eliminating cross-contamination risk and enabling safe handling of sensitive reagents, sterile formulations, and aggressive solvents without wetted metal parts.

Key Features

• High-accuracy dispensing with typical volumetric error ≤ ±0.5% under calibrated conditions using recommended tubing (e.g., silicone or PharMed® BPT), validated via gravimetric measurement per ISO 8655-3 guidelines.
• Programmable dispensing parameters including target volume (0.1 mL–500 mL), dispense time (1.5–4 s), retract angle (0°–30°), acceleration/deceleration profiles, and batch quantity — all accessible via intuitive bilingual LCD interface.
• Dual-mode operation: standalone mode with local keypad control and external mode via RS485 serial interface supporting Modbus RTU protocol for integration into PLC-controlled filling lines or LIMS-hosted workflows.
• Robust mechanical architecture featuring CNC-machined aluminum alloy chassis, electrophoretic-coated steel enclosure, and IP20-rated front panel — designed for routine cleaning with 70% ethanol or isopropyl alcohol and resistance to common laboratory corrosives.
• Process safety functions include no-bottle detection (via optional photoelectric sensor input), automatic purge cycle, power-fail memory retention (preserves last 100 batch records and active settings), and configurable alarm outputs for over-torque, tubing wear, or communication timeout.

Sample Compatibility & Compliance

The WF600G-YZ35 accommodates standard peristaltic pump tubing sizes compatible with YZ35 roller geometry, including but not limited to Norprene® LFT-30, Masterflex® I/P 16, and Pharmapure® 204010085/86 series (ID 3.2 mm, wall thickness 1.6 mm). It supports aqueous buffers, cell culture media, diagnostic reagents, lyophilized powder suspensions, and low-concentration ethanol/water mixtures (≤30% v/v). While not certified as a Class A medical device, its design aligns with key elements of ISO 13485:2016 (for manufacturers of medical device accessories) and supports GMP-compliant documentation practices through audit-ready calibration logs and parameter change history. Optional 21 CFR Part 11-compliant software add-ons are available for electronic signature, user access control, and immutable event logging.

Software & Data Management

The onboard firmware stores up to 100 batch records with timestamps, total dispensed volume, average flow rate, and error flags. Via RS485, users can export raw dispense events (start/stop time, pulse count, retract status) to SCADA systems or custom Python/Node-RED scripts for real-time dashboarding. PreFluid provides a Windows-based calibration utility for gravimetric verification, generating PDF reports with uncertainty estimates per EURACHEM/CITAC Guide. Firmware updates are delivered via USB flash drive with SHA-256 checksum validation to ensure integrity.

Applications

• Pharmaceutical aseptic filling: precise volumetric dosing of water-for-injection (WFI) into vials prior to lyophilization (USP ).
• In vitro diagnostic (IVD) manufacturing: metered dispensing of enzyme conjugates, calibrators, and controls into microtiter plates or assay cartridges (CLSI EP10-A3 compliant workflows).
• Bioprocess intermediate transfer: controlled addition of pH adjusters, antifoam agents, or feed solutions into bioreactors during fed-batch cultivation.
• Academic research labs: reproducible sample aliquoting for HPLC autosampler vials, PCR plate setup, or multi-well cytotoxicity assays requiring sub-milliliter precision.

FAQ

What tubing sizes are compatible with the YZ35 roller assembly?
Standard tubing with inner diameter 3.2 mm and wall thickness 1.6 mm (e.g., Pharmapure 204010085) is recommended for optimal occlusion and longevity. Other sizes may be used but require recalibration and may reduce accuracy or tube life.
Does the pump support FDA 21 CFR Part 11 compliance?
The base unit does not include electronic signature or audit trail functionality; however, it interfaces with third-party Part 11-compliant SCADA or MES platforms via Modbus RTU for full regulatory traceability.
How is accuracy verified and maintained over time?
Accuracy is verified gravimetrically using NIST-traceable analytical balances and documented per internal SOPs. Routine maintenance includes visual inspection of tubing compression, roller wear, and periodic recalibration every 6 months or after 500 operating hours.
Can the pump operate continuously at maximum speed (600 rpm)?
Yes, continuous operation at 600 rpm is supported for short durations (<30 min); extended high-speed use requires active cooling and tubing replacement every 12–24 hours depending on fluid chemistry and ambient temperature.
Is there an option for analog (0–10 V or 4–20 mA) speed control?
No analog input is provided natively; speed modulation must be achieved via Modbus register writes or front-panel programming. External analog-to-serial converters may be integrated with appropriate isolation.