Revvity Sciclone G3 NGSx High-Throughput Automated Library Preparation Workstation
| Brand | Revvity |
|---|---|
| Origin | USA |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Product Category | Imported Instrument |
| Model | Sciclone G3 NGSx High-Throughput Automated Library Preparation Workstation |
| Sample Throughput | 8–96 samples per run |
Overview
The Revvity Sciclone G3 NGSx High-Throughput Automated Library Preparation Workstation is an integrated, benchtop liquid handling platform engineered specifically for reproducible, walk-away automation of next-generation sequencing (NGS) library construction workflows. Leveraging precise positive-displacement pipetting and modular deck architecture, the system executes complex multi-step protocols—including fragmentation, end repair, A-tailing, adapter ligation, size selection, and PCR amplification—under fully programmable control. Designed to meet the stringent demands of core genomics facilities, clinical research labs, and contract research organizations (CROs), the Sciclone G3 NGSx operates within defined thermal and environmental constraints to ensure consistent enzymatic reaction kinetics and nucleic acid integrity across runs. Its closed-system workflow minimizes aerosol generation and operator-dependent variability, directly supporting compliance with ISO/IEC 17025 quality management requirements for molecular testing laboratories.
Key Features
- Benchtop footprint with recessed deck design accommodating up to 24 tip boxes (96 tips/box), reducing X-Y-Z axis travel and enhancing mechanical stability during high-cycle operations.
- Integrated gripper arm with full positional control, enabling seamless plate movement between deck modules without reliance on external stackers or robotic conveyors.
- Pre-validated, vendor-agnostic protocol library covering major NGS chemistries—including Illumina, Thermo Fisher Ion Torrent, Oxford Nanopore, and PacBio—supporting WGS, RNA-Seq, ChIP-Seq, targeted panels, and amplicon sequencing.
- Filter-tip compatibility across all pipetting channels to eliminate cross-contamination risk in low-input and single-cell applications.
- Onboard temperature-controlled modules (4°C–70°C) for reagent storage, incubation, and post-ligation cleanup steps, eliminating manual plate transfers.
- Real-time error logging and hardware diagnostics integrated into the control software, supporting GLP-compliant audit trails and instrument qualification (IQ/OQ).
Sample Compatibility & Compliance
The Sciclone G3 NGSx supports input materials ranging from purified genomic DNA and total RNA to FFPE-derived nucleic acids and cfDNA, with compatibility across standard 96-well and 384-well microplate formats. All liquid handling steps adhere to CLSI EP25-A guidelines for verification of automated nucleic acid extraction and library prep systems. The platform is validated for use in environments governed by FDA 21 CFR Part 11 (electronic records/signatures), supporting role-based user access, electronic signature capture, and immutable audit logs. It meets IEC 61000-6-3 EMC emission standards and UL 61010-1 safety certification for laboratory instrumentation.
Software & Data Management
Controlled via Revvity’s intuitive Sciclone Control Software v5.x, the workstation enables drag-and-drop protocol assembly, parameter validation, and real-time run monitoring through a web-enabled interface. Protocol files include embedded metadata (reagent lot numbers, expiration dates, calibration timestamps) and support version-controlled deployment across multiple instruments. Raw execution logs are exportable in CSV and XML formats for integration with LIMS platforms (e.g., LabVantage, STARLIMS) and downstream bioinformatics pipelines. Software updates are delivered through secure, authenticated channels compliant with NIST SP 800-53 security controls.
Applications
- High-volume clinical NGS testing workflows requiring batch processing of 8–96 samples per run with minimal hands-on time.
- Multi-omic core facilities performing parallel library prep for DNA, RNA, and epigenetic assays under standardized SOPs.
- Pharmaceutical development labs executing GMP-aligned library prep for biomarker discovery and companion diagnostic assay validation.
- Academic sequencing centers scaling throughput while maintaining inter-run CVs ≤8% for fragment size distribution and library yield metrics.
- Reference laboratories implementing ISO 15189-accredited NGS testing where traceability, reagent tracking, and environmental monitoring are mandatory.
FAQ
What types of NGS library preparation kits are supported out-of-the-box?
The system ships with pre-verified methods for Illumina TruSight, Nextera Flex, and IDT xGen; Thermo Fisher AmpliSeq and Ion Chef; and Oxford Nanopore SQK kits—all accessible via the protocol library browser.
Can the Sciclone G3 NGSx be integrated into an existing LIMS environment?
Yes—through RESTful API endpoints and configurable HL7/CSV data export modules, enabling bidirectional sample tracking and result synchronization.
Is routine maintenance required between runs?
No scheduled maintenance is needed between runs; however, daily tip box replacement and weekly deck surface decontamination using 70% ethanol are recommended per Revvity’s operational guidance.
How does the system handle low-input or degraded samples (e.g., FFPE or cfDNA)?
The platform supports dual-channel low-volume pipetting (0.5–200 µL) with positive displacement tips, and optional magnetic bead-based cleanup modules optimized for sub-10 ng inputs.
Does Revvity provide application support for custom protocol development?
Yes—Revvity’s Global Applications Team offers remote and on-site protocol adaptation services, including analytical method transfer, robustness testing, and IQ/OQ documentation support.
