Sanotac SW100 Automated Column Chromatography System for Preparative Liquid Chromatography
| Brand | Sanotac |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Manufacturer |
| Origin Category | Domestic |
| Model | SW100 |
| Application Level | Laboratory Grade |
| Instrument Type | High-Pressure Preparative Liquid Chromatograph |
| Flow Rate Range | 0.01–100.00 mL/min |
| Flow Accuracy | ±1% |
| Flow Precision (RSD) | ≤0.5% |
| Maximum Pressure | 10 MPa |
| Wavelength Range | 190–800 nm |
| Wavelength Accuracy | ±1 nm |
| Wavelength Repeatability | 0.2 nm |
| Baseline Noise | ±0.5 × 10⁻⁵ AU (254 nm, TC = 1 s) |
| Data Acquisition Frequency | 10 Hz |
| Gradient Types | Step, Linear, Online Adjustable |
| UV Detector | Dual-lamp (Deuterium + Tungsten), Simultaneous Dual-Wavelength Detection |
| Fraction Collector | Automated, 2 × 60 positions (Φ15 mm × 150 mm tubes), Time/Peak/Threshold/Sequential/Cyclic Collection Modes |
| System Materials | 316L Stainless Steel, PEEK, Titanium, Hastelloy options |
| Software Compliance | Fully compliant with CFDA GxP and FDA 21 CFR Part 11 (audit trail, electronic signature, role-based access control, database-driven project management) |
Overview
The Sanotac SW100 Automated Column Chromatography System is a high-pressure preparative liquid chromatography (PLC) platform engineered for reproducible, scalable purification of small-molecule compounds, natural products, synthetic intermediates, and biomolecules—including peptides and moderately sized proteins. Operating on the fundamental principle of differential partitioning between a mobile phase and a stationary phase under controlled pressure gradients, the SW100 delivers precise solvent delivery, real-time dual-wavelength UV-Vis detection, and synchronized fraction collection within a single integrated architecture. Designed for laboratory-scale method development and gram-to-hundred-gram preparative workflows, it bridges the gap between analytical HPLC optimization and process-scale purification—enabling seamless transition from discovery to preclinical material supply. Its modular design supports both stainless-steel and high-pressure glass columns (up to 10 MPa), accommodating diverse stationary phases including reversed-phase C18, silica, amino, cyano, and ion-exchange media.
Key Features
- High-precision dual-plunger reciprocating pump with cam-profile pulsation compensation, delivering flow stability with RSD ≤ 0.5% across the full range (0.01–100.00 mL/min) and accuracy of ±1%.
- Dual-lamp UV-Vis detector (deuterium + tungsten) enabling simultaneous monitoring at two user-selectable wavelengths (190–800 nm), with wavelength accuracy ±1 nm and repeatability of 0.2 nm—critical for co-elution assessment and impurity tracking.
- Low-noise optical system: baseline noise ≤ ±0.5 × 10⁻⁵ AU at 254 nm (1 s time constant), ensuring reliable peak integration even for trace-level components.
- Automated fraction collector with programmable collection logic—including time-based, peak-triggered, threshold-defined, sequential, and cyclic modes—supporting standard 15 mm × 150 mm test tubes (2 × 60 positions) and customizable rack configurations.
- Self-cleaning pump head design minimizes salt crystallization and buffer residue accumulation, extending maintenance intervals and improving long-term system robustness.
- Multi-material fluidic path options (316L stainless steel, PEEK, titanium, Hastelloy C-276) ensure chemical compatibility with aggressive solvents (e.g., TFA, HFIP), acidic/basic mobile phases, and protein-denaturing conditions.
- Modular expandability: compatible with optional detectors (ELSD, RI), external sample injectors, column switching valves, and SPE cartridge interfaces—without hardware modification.
Sample Compatibility & Compliance
The SW100 accommodates a broad spectrum of sample matrices: crude plant extracts, combinatorial synthesis libraries, fermentation broths, and recombinant protein lysates. It supports both liquid and solid-phase loading via a 10 mL fixed-loop preparative injection valve. Column compatibility includes standard 10–50 mm ID stainless-steel columns and reinforced glass columns rated to 10 MPa—enabling use of sub-10 µm particles for enhanced resolution. All operational parameters—including gradient profiles, flow rates, detection settings, and collection criteria—are stored in a relational database schema per project, ensuring full traceability. The system adheres to regulatory requirements for data integrity: electronic records are immutable, user actions are logged with timestamps and operator IDs, and role-based permissions enforce separation of duties. Audit trails comply with FDA 21 CFR Part 11 and CFDA GxP guidelines, supporting GLP/GMP-aligned laboratories engaged in API purification, botanical standardization, or biopharmaceutical process characterization.
Software & Data Management
Controlled via Sanochrom™ Chromatography Workstation (Windows 10/8/7 compatible), the SW100 software provides a graphical method editor, real-time chromatogram display, and integrated fraction mapping. Methods are saved as version-controlled objects linked to raw data files, calibration logs, and instrument configuration snapshots. Project-level data storage ensures that every chromatographic run—including pump parameters, UV spectra, and collected fractions—is contextually associated with its experimental intent. The software implements cryptographic hash verification for data files, enforces mandatory electronic signatures for method activation and report generation, and maintains tamper-evident audit logs covering login events, parameter changes, and data exports. Remote monitoring via secure USB or RS-232 interface enables supervised operation without local workstation dependency.
Applications
- Purification of bioactive alkaloids, flavonoids, and terpenoids from traditional Chinese medicine extracts.
- Isolation of chiral intermediates and stereoisomeric APIs following asymmetric synthesis.
- Desalting and buffer exchange of peptide fractions prior to lyophilization.
- Removal of endotoxins and host-cell proteins during early-stage monoclonal antibody purification.
- Fractionation of oligonucleotide synthesis crude mixtures (e.g., phosphoramidite deprotection byproducts).
- Preparative separation of polymer additives, catalyst residues, and reaction side-products in fine chemical manufacturing.
FAQ
Does the SW100 support method transfer from analytical HPLC to preparative scale?
Yes—gradient slope normalization, retention time scaling, and column volume ratio calculations are supported within Sanochrom™ software to facilitate robust method translation.
Can the system be validated for GMP environments?
The SW100 meets core instrumentation qualification requirements (IQ/OQ/PQ) and includes built-in tools for flow rate verification, wavelength calibration, and detector linearity testing—documented in the provided validation protocol templates.
Is third-party column compatibility guaranteed?
All standard 1/4″–28 UNF and M10 × 1 fittings are supported; column hardware adapters and pressure-rated unions are supplied for seamless integration with columns from Waters, Agilent, Phenomenex, YMC, and other major vendors.
What maintenance routines are recommended for extended uptime?
Daily pump seal flush with water/methanol, weekly UV lamp intensity check, quarterly precision filter replacement, and biannual piston seal inspection—detailed in the preventive maintenance logbook included with shipment.
