Shanghai Shiping SPH-200F Advanced Temperature-Controlled Orbital Shaker

Overview

The Shanghai Shiping SPH-200F Advanced Temperature-Controlled Orbital Shaker is a benchtop incubatory shaker engineered for precise, reproducible cultivation of microbial, mammalian, and suspension cell cultures under tightly regulated thermal and mechanical conditions. It operates on the principle of orbital shaking—generating uniform circular motion with a fixed 25 mm diameter—to ensure consistent oxygen transfer, nutrient homogenization, and minimal shear stress across culture vessels. Integrated P.I.D. microprocessor control enables dynamic regulation of both temperature and rotational speed, maintaining setpoints within ±0.2 °C and ±1 rpm respectively. The unit features a forced-air circulation system with independent heating (400 W) and R134a-based refrigeration (130 W), enabling stable operation from 4 °C (refrigerated culturing) up to 60 °C (thermophilic or enzyme assay applications). Its 69 L chamber accommodates standard flask configurations—including up to twenty-five 100 mL flasks or nine 1 L vessels—making it suitable for small-scale bioprocess development, QC microbiology, and academic research labs requiring GxP-aligned environmental control.

Key Features

• Single-axis balanced drive mechanism ensures low-vibration, high-stability orbital motion with no mechanical backlash or resonance at any speed (30–400 rpm).
• Full-range amplitude (25 mm orbital diameter) optimized for enhanced dissolved oxygen transfer in aerobic cultures without compromising cell viability.
• Dual-mode operation: switchable between continuous orbital and reciprocating motion via firmware toggle—enabling method flexibility for varied suspension requirements.
• LCD backlit display with dual-parameter readout (setpoint vs. real-time temperature & rpm), intuitive navigation, and password-protected parameter locking to prevent unauthorized changes.
• Comprehensive safety architecture: independent overtemperature cutoff (mechanical + electronic), motor overheat protection, refrigeration overload monitoring, ground-fault circuit interruption (GFCI), and sensor fault diagnostics.
• Intelligent power recovery logic resumes pre-interruption settings—including timer, temperature, and speed—following AC power loss, supporting unattended overnight runs.
• NSK double-row precision bearings and heat-treated 45# carbon steel shaft ensure >20,000 hr operational lifetime with zero maintenance under continuous duty cycles.

Sample Compatibility & Compliance

The SPH-200F supports standard Erlenmeyer flasks (50–2000 mL), test tubes, deep-well plates, and custom bioreactor vessels mounted on its 420 × 400 mm 304 stainless steel platform. Chamber geometry (460 × 500 × 350 mm internal) permits vertical clearance up to 990 mm when fully opened—facilitating safe handling of tall culture vessels. All wetted surfaces are electropolished 304 stainless steel; non-wetted housing uses corrosion-inhibiting ABS polymer compliant with UL 94 V-0 flame rating. While not certified to ISO 13485 or FDA 21 CFR Part 11 out-of-the-box, the instrument’s audit-trail-capable parameter logging (via optional RS-485/Modbus interface), encrypted settings, and automatic event alarms support alignment with GLP and GMP documentation requirements when integrated into validated lab workflows.

Software & Data Management

The embedded microcontroller provides local data retention for up to 100 runtime events (e.g., temperature excursions, door openings, alarm triggers) with timestamps. Optional serial communication (RS-485) enables integration with LabVantage, STARLIMS, or custom SCADA systems for centralized monitoring and CSV export. Firmware supports eight-segment programmable ramp profiles—ideal for simulating diurnal temperature shifts or gradual acclimatization protocols. Real-time clock synchronization, parameter memory recall, and one-touch factory reset further streamline SOP-driven operations in regulated environments.

Applications

• Microbial growth kinetics studies (E. coli, yeast, Bacillus spp.) under controlled hypothermic or thermogenic conditions.
• Suspension-based mammalian cell culture (CHO, HEK293) during transfection optimization and early-stage bioprocess screening.
• Enzyme activity assays requiring precise thermal equilibration and agitation (e.g., lipase, amylase, protease kinetics).
• Environmental microbiology: water quality testing, BOD incubation, and antibiotic susceptibility profiling per CLSI M07-A10 guidelines.
• Pharmaceutical QC: stability-indicating dissolution testing, extractables/leachables sample agitation, and media preparation validation.

FAQ

Does the SPH-200F support external data logging or network connectivity?
Yes—via optional RS-485 Modbus RTU interface, enabling bidirectional communication with LIMS or building management systems.
Can the unit maintain 4 °C while operating at 400 rpm?
Yes—the refrigeration system (130 W R134a compressor) and insulated chamber allow simultaneous low-temperature and high-speed operation without thermal drift.
Is calibration traceable to NIST or other national standards?
Factory calibration certificates are provided for temperature (±0.1 °C accuracy) and speed (±1 rpm); users may perform field verification using ISO/IEC 17025-accredited reference probes.
What is the maximum flask fill volume supported at 400 rpm without splashing?
For 250 mL flasks, ≤60% fill volume is recommended; for 1 L flasks, ≤40% fill volume ensures containment integrity across the full speed range.
Are replacement parts such as bearings, compressors, or control boards available globally?
Original NSK bearings, Danfoss compressors, and proprietary PCB assemblies are stocked by Shanghai Shiping’s international distribution partners with lead times under 4 weeks.