Shanghai Shiping SPH-300D/311D/322D Single-Layer Orbital Shaker (50 mm Orbit Diameter)

Overview

The Shanghai Shiping SPH-300D/311D/322D series are single-layer orbital shakers engineered for high-reliability microbial cultivation in life science and biopharmaceutical R&D environments. These instruments operate on the principle of orbital motion—rotating the platform in a circular path with a fixed diameter (Φ50 mm)—to generate controlled shear and gas–liquid interfacial dynamics essential for aerobic fermentation. Unlike reciprocating or linear shakers, orbital motion promotes uniform liquid film formation along vessel walls, significantly increasing the effective surface area for oxygen transfer (k_La). This is especially critical for antibiotic-producing strains (e.g., Streptomyces spp.) and other obligate aerobes requiring sustained dissolved oxygen (DO) levels during extended batch culture. The SPH series is designed for integration into climate-controlled rooms where ambient temperature stability is maintained externally; it does not incorporate active heating or cooling, ensuring energy efficiency and mechanical simplicity.

Key Features

• PID microprocessor control system with LCD interface enabling precise, repeatable speed regulation (±1 rpm accuracy) across the full 50–300 rpm range.
• Eight-segment programmable operation: each segment supports independent speed and duration settings, facilitating complex multi-phase cultivation protocols (e.g., lag-phase adaptation followed by exponential growth optimization).
• Patented single-axis balanced drive mechanism—designed and manufactured in China—ensures low-vibration, low-noise operation and long-term mechanical stability under continuous duty cycles.
• Soft-start circuitry prevents abrupt acceleration, minimizing splashing and preserving sample integrity, particularly in low-volume cultures (e.g., 50 mL in 500 mL Erlenmeyer flasks).
• Dual-layer safety architecture: independent over-speed detection with audible/visual alert, plus redundant electrical protection (leakage current cutoff, over-current trip, and motor thermal shutdown).
• Robust mechanical construction using cold-rolled steel chassis, structural cast iron supports, chromium-alloy drive shafts, and premium-grade imported bearings for >10,000 hours of maintenance-free service life.
• Electrostatically applied corrosion-resistant powder coating ensures durability in humid laboratory environments and complies with ISO 9001-certified manufacturing practices.

Sample Compatibility & Compliance

The SPH-300D/311D/322D accommodates standard microbiological vessels including glass and polycarbonate Erlenmeyer flasks (50–2000 mL), test tubes in rack holders, and shallow culture dishes. Its Φ50 mm orbit diameter is optimized for scalable process development—from screening-level 250 mL cultures to pilot-scale 2 L batches—while maintaining consistent k_La profiles across vessel sizes. The shaker meets IEC 61010-1:2010 safety requirements for laboratory equipment and incorporates design elements aligned with GLP-compliant workflows, including parameter memory retention, power-fail recovery, and auditable operational logs (when interfaced with external data acquisition systems). While not certified for GMP production environments, its mechanical reproducibility and traceable speed calibration support method validation per USP <1058> and ASTM E2500-13 guidelines.

Software & Data Management

These models feature embedded firmware—not cloud-connected software—with local data persistence: all user-programmed parameters (speed, time, segment sequence) are retained after power cycling. The LCD display provides real-time feedback on setpoint, actual speed, elapsed time, and remaining duration. For laboratories requiring electronic records, optional RS-232 or analog 0–10 V output interfaces enable connection to SCADA systems or LIMS platforms. Audit trail functionality (e.g., FDA 21 CFR Part 11 compliance) is achievable via third-party data loggers that capture timestamped speed and runtime events, supporting regulatory submissions for preclinical fermentation studies.

Applications

• Aerobic microbial strain screening and optimization in antibiotic discovery pipelines.
• Recombinant protein expression in E. coli and yeast systems requiring tight DO control.
• Cell culture support for suspension-adapted mammalian lines during early-process characterization.
• Enzyme production and metabolic engineering studies where oxygen mass transfer limits productivity.
• Environmental microbiology assays involving hydrocarbon-degrading consortia or nitrifying bacteria.
• Academic teaching labs conducting fundamental studies in microbial kinetics and bioreactor scaling principles.

FAQ

Does this shaker include temperature control?
No. The SPH-300D/311D/322D operates at ambient temperature only and must be installed in a thermostatically regulated room or incubator chamber.
What is the maximum flask size supported on the SPH-322D platform?
The 1108 × 740 mm platform accommodates up to four 2 L Erlenmeyer flasks or twelve 500 mL flasks using standard universal clamps.
Is speed calibration traceable to national standards?
Yes—each unit undergoes factory calibration using a NIST-traceable tachometer; calibration certificates are available upon request.
Can the shaker run unattended for 500 hours?
Yes. The 0–500 h timer supports fully autonomous operation, and the variable-frequency motor is rated for continuous duty under nominal load conditions.
Are replacement parts such as bearings or drive belts available?
All critical wear components—including imported bearings, motor assemblies, and control PCBs—are stocked and supplied directly by Shanghai Shiping’s international spare parts division.