TIANLONG PANA3200S+ Fully Automated Magnetic Bead-Based Nucleic Acid Purification System
| Brand | TIANLONG |
|---|---|
| Origin | Shaanxi, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Instrument Type | Magnetic Bead-Based Purification |
| Automation Level | Dedicated Fully Automatic |
| Throughput | 32 samples per run |
| Compliance | CE-IVD compliant (as per EU IVDR Annex II Class B), GLP/GMP-ready architecture |
Overview
The TIANLONG PANA3200S+ is a fully integrated, walk-away nucleic acid purification system engineered for high-throughput clinical diagnostics and molecular biology laboratories. It operates on the principle of magnetic bead-based solid-phase extraction—leveraging paramagnetic particles functionalized with silica or carboxyl groups to selectively bind nucleic acids under controlled chaotropic salt and pH conditions. The system automates lysis, binding, washing, elution, and optional downstream PCR reaction setup in a single platform when interfaced with the Gentier 96E or 96R real-time PCR systems. Designed for diagnostic-grade reproducibility, it supports end-to-end traceability from sample tube scanning to purified nucleic acid output, minimizing manual intervention and human error while maintaining strict biosafety containment.
Key Features
- Fully automated workflow: From barcode-scanned sample loading through magnetic bead separation, wash cycles, and elution to optional PCR master mix dispensing—executed without operator intervention.
- Patented rotary mixing technology (CN ZL201710435931.8): Ensures uniform magnetic bead suspension and maximizes nucleic acid recovery across viscous or particulate-laden samples such as whole blood, nasopharyngeal swab eluates, urine, and tissue homogenates.
- Integrated biosafety architecture: Includes laminar airflow with HEPA filtration (H14 grade), negative-pressure chamber design, directional exhaust ducting, UV-C germicidal irradiation (254 nm), droplet capture trays, and disposable waste cassettes compliant with WHO BSL-2 and ISO 15195:2018 requirements.
- Cold reagent storage: On-board 4–10 °C refrigerated reagent compartment maintains stability of enzymes, lysis buffers, and elution solutions during extended runs.
- Precision liquid handling: Dual-channel positive-displacement pipetting with 1–1000 µL range, calibrated to ISO 8655-6 standards; centrifugation module (up to 3000 ×g) integrated for post-elution clarification.
Sample Compatibility & Compliance
The PANA3200S+ accepts primary collection tubes—including 10–15 mL conical tubes, 5 mL EDTA tubes, and standard U-bottom 96-well plates—without decanting or transfer. It supports direct input of diverse clinical matrices: anticoagulated whole blood, bronchoalveolar lavage fluid, cerebrospinal fluid, saliva, urine, and flocked nylon swab transport media. All purification protocols are validated per ISO 20387:2018 (biobanking) and align with CLSI EP12-A2 and EP25-A guidelines for verification of nucleic acid extraction performance. The system’s software logs full audit trails meeting FDA 21 CFR Part 11 requirements for electronic records and signatures, including user authentication, timestamped event logging, and immutable data export in CSV and PDF formats.
Software & Data Management
Controlled via a Windows-based embedded HMI with touchscreen interface, the PANA3200S+ software provides real-time status monitoring, protocol customization (including user-defined incubation times, wash volumes, and elution temperatures), and dynamic error recovery. Each run generates a comprehensive digital report containing sample IDs, instrument parameters, reagent lot numbers, environmental sensor readings (temperature/humidity), and QC flagging for low-yield or inhibited extractions. Data export supports LIS/HIS integration via ASTM E1384-compliant HL7 v2.5.1 messaging and secure SFTP transmission. Software validation documentation (IQ/OQ/PQ) is provided per IEC 62304 Class B medical device software standards.
Applications
The system serves core applications in clinical virology (e.g., SARS-CoV-2, influenza A/B, RSV detection), oncology (circulating tumor DNA isolation), transplant monitoring (donor-derived cell-free DNA quantification), and antimicrobial resistance profiling (carbapenemase gene screening). Its throughput and compatibility make it suitable for centralized reference labs processing >200 samples/day, public health surveillance programs requiring rapid turnaround, and research cores supporting CRISPR library prep or single-cell RNA-seq workflows where input consistency directly impacts sequencing library complexity.
FAQ
What sample types are validated for use with the PANA3200S+?
Whole blood (EDTA/K2EDTA), nasopharyngeal/oropharyngeal swabs in viral transport media, urine, saliva, and formalin-fixed paraffin-embedded (FFPE) tissue lysates—all supported with protocol-specific optimization kits.
Does the system support regulatory submissions for IVD assays?
Yes. The platform is CE-IVD marked (Annex II Class B), and its design history file, risk management report (ISO 14971:2019), and verification protocols are available to support FDA 510(k) or PMDA approval pathways.
Can the PANA3200S+ be integrated into existing laboratory automation systems?
It features RS232, USB, and Ethernet interfaces with open API support for middleware integration (e.g., STARLIMS, LabWare LIMS) and robotic arm handoff via standardized gripper positioning coordinates.
How is carryover contamination prevented between runs?
Each run includes automated tip ejection into sealed waste cassettes, HEPA-filtered air purging of pipette manifolds, and UV-C exposure of the deck surface for ≥15 minutes post-run—validated per CLSI MM18-A to achieve <1 copy/µL cross-contamination threshold.
