UC-3220A Integrated High-Performance Liquid Chromatograph
| Origin | Beijing, China |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Origin Category | Domestic (PRC) |
| Model | UC-3220A |
| Instrument Type | Conventional HPLC System |
| Flow Rate Range | 0.001–9.999 mL/min |
| Pump Configuration | Dual-piston serial design with electronic pressure pulsation suppression |
| Flow Accuracy | <0.4% RSD (1 mL/min, deionized water, 25 °C) |
| Flow Precision | 0.1% RSD (1 mL/min, deionized water, 25 °C) |
| Maximum Pressure | 40 MPa (1 mL/min, deionized water, 25 °C) |
| Pressure Pulsation | <0.05 MPa (1 mL/min, deionized water, 25 °C) |
| Gradient Accuracy | ±1% |
| Autosampler | XYZ robotic arm configuration (standard) |
| Sample Capacity | 120 × 1.8 mL vials |
| Sample Tray Temperature Control | 4–40 °C |
| Injection Precision | 0.5% RSD (254 nm, 20 µL naphthalene standard in methanol, 1 mL/min) |
| UV-Vis Wavelength Range | 190–740 nm |
| Light Source | Deuterium lamp |
| Wavelength Accuracy | ±1 nm |
| Spectral Scanning Mode | Stop-flow automatic scan |
| Baseline Noise | 2 × 10⁻⁵ AU (254 nm, 25 °C) |
| Baseline Drift | 15 × 10⁻⁵ AU/h (254 nm, 25 °C) |
| LOD | 5 × 10⁻⁹ g/mL (254 nm, naphthalene standard in methanol, 25 °C) |
| Communication Interface | RS-232 for full bidirectional instrument control |
| Display | 4.3-inch TFT color touchscreen |
| Power Supply | 220 V AC, 3 A |
| Dimensions (W × H × D) | 320 × 430 × 430 mm |
| Weight | 25 kg |
Overview
The UC-3220A Integrated High-Performance Liquid Chromatograph is a fully self-contained, benchtop HPLC system engineered for precision, reproducibility, and operational autonomy in regulated and research laboratory environments. Built upon classical reversed-phase, normal-phase, and ion-exchange chromatographic principles, the system employs high-pressure liquid delivery, UV-Vis absorbance detection, and thermally controlled column and sample environments to resolve, identify, and quantify analytes across pharmaceutical, environmental, food safety, and academic applications. Its monolithic architecture integrates dual high-pressure pumps, a variable-wavelength UV-Vis detector, a 120-position temperature-regulated autosampler, and a programmable column oven—eliminating external cabling, inter-unit synchronization delays, and mechanical alignment dependencies typical of modular HPLC configurations. The system operates under full computer control via RS-232 interface, enabling seamless integration into laboratory information management systems (LIMS) and supporting GLP/GMP-compliant workflows through audit-trail-capable software.
Key Features
- Monolithic integrated design reduces footprint and inter-component variability, enhancing method transfer reliability between laboratories.
- Dual-piston serial pump architecture with real-time electronic pressure pulsation suppression—replacing mechanical dampeners—ensures stable flow delivery across the full 0.001–9.999 mL/min range and improves long-term pump longevity.
- UV-Vis detector features digitally processed analog-to-digital conversion, wavelength programmability (including time-based wavelength stepping), and stop-flow spectral scanning for peak purity assessment and method development support.
- XYZ robotic autosampler supports precise, low-memory carryover injection (0.5% RSD) and accommodates up to 120 standard 1.8 mL vials; optional 4–40 °C tray cooling minimizes thermal degradation of labile compounds.
- Column oven provides uniform, programmable temperature control (ambient to 80 °C, depending on configuration), critical for retention time stability and method robustness.
- 4.3-inch color touchscreen interface enables local method setup, diagnostic self-tests, and real-time parameter monitoring without external PC dependency.
- Full bidirectional instrument control via dedicated chromatography workstation software—including gradient programming, sequence definition, data acquisition, peak integration, calibration curve generation, and report export in PDF/CSV formats.
Sample Compatibility & Compliance
The UC-3220A supports a broad range of sample matrices including aqueous extracts, organic solvent solutions, biological fluids (after appropriate preparation), and formulated pharmaceutical products. It is compatible with standard 3.0–4.6 mm ID analytical columns packed with C18, C8, phenyl-hexyl, HILIC, or ion-exchange phases. System compliance aligns with foundational analytical standards including ASTM E260 (Standard Practice for Calibration of Gas Chromatographs), ISO 17025 (General requirements for the competence of testing and calibration laboratories), and USP (Chromatography). When operated with validated methods and enabled audit-trail functionality, the platform meets documentation requirements for FDA 21 CFR Part 11 compliance in regulated QC/QA settings.
Software & Data Management
The UC-3220A is controlled by a dedicated Windows-based chromatography data system (CDS) supporting full instrument parameterization—including pump gradient profiles, detector wavelength programs, autosampler sequences, and column oven temperature ramps. Raw data are stored in vendor-neutral .CDF format (compatible with third-party processing tools), while processed results include annotated chromatograms, peak tables with retention time, area, height, and %RSD values. Software features include multi-user access control, electronic signature capability, version-controlled method storage, and automated backup scheduling. All instrument events—including initialization, error logs, maintenance alerts, and user actions—are timestamped and retained in an immutable audit trail file, satisfying traceability requirements for regulatory submissions and internal quality audits.
Applications
- Pharmaceutical QC: Assay and impurity profiling of active pharmaceutical ingredients (APIs) per ICH Q2(R2) guidelines.
- Environmental analysis: Quantification of PAHs, pesticides, and endocrine disruptors in water and soil extracts.
- Food and beverage testing: Detection of preservatives (e.g., benzoic acid, sorbic acid), artificial colors, caffeine, and mycotoxins.
- Academic research: Method development for chiral separations, peptide mapping, and natural product isolation.
- Biotechnology: Monitoring of monoclonal antibody fragments, glycoprotein variants, and excipient stability studies.
FAQ
Is the UC-3220A compatible with UHPLC columns?
The system is rated for a maximum pressure of 40 MPa (5800 psi), making it suitable for conventional HPLC columns (up to 300 Å pore size, 3–5 µm particles) but not optimized for sub-2 µm UHPLC columns requiring >60 MPa operation.
Can the system be upgraded to quaternary gradient capability?
Yes—the base configuration supports binary high-pressure gradient mixing; optional low-pressure quaternary solvent selection modules are available as field-installable kits.
Does the software support 21 CFR Part 11 compliance out of the box?
Audit trail logging, electronic signatures, and user role management are built-in; however, full Part 11 compliance requires site-specific validation documentation and administrative policy implementation.
What maintenance intervals are recommended for the pump and detector?
Pump seal replacement is advised every 6–12 months based on usage intensity; detector lamp life is typically 1000–2000 hours, with automatic usage tracking embedded in the software.
Is remote monitoring supported?
While native remote desktop access is not provided, the RS-232 interface allows integration with third-party SCADA or LIMS platforms for status polling and alarm notification via TCP/IP gateways.
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