Zequan HSP200 Portable Bioaerosol Sampler + ICT-208-16-YR Isothermal Fluorescent Nucleic Acid Analyzer for SARS-CoV-2 Detection in Ambient Air

Overview

The Zequan HSP200–ICT-208-16-YR integrated bioaerosol detection system is engineered for field-deployable, nucleic acid–based identification of SARS-CoV-2 in ambient air. It implements a two-stage workflow grounded in standardized aerosol science and isothermal amplification: first, the HSP200 portable wet-wall cyclone sampler collects viable and non-viable viral particles ≥1.0 µm from air using inertial impaction principles; second, the ICT-208-16-YR performs real-time fluorescent detection of amplified viral RNA fragments without requiring thermal cycling or nucleic acid extraction. This architecture aligns with ISO/IEC 17025–compatible workflows and supports rapid, decentralized surveillance in non-laboratory environments—such as transportation hubs, schools, healthcare waiting areas, and congregate living facilities—where biosafety level 3 (BSL-3) infrastructure is unavailable.

Key Features

• Wet-wall cyclone sampling technology compliant with GB/T 38517–2020 and GB/T 39990–2021, ensuring reproducible particle capture efficiency ≥90% for ≥1.0 µm bioaerosols
• High-flow sampling (>200 L/min) enabling representative air volume acquisition in under 10 minutes per site
• Integrated liquid-phase collection: proprietary SL-series viral preservation buffer forms a dynamic film on the cyclone wall, stabilizing RNA integrity during transport and storage
• True point-of-care operation: ICT-208-16-YR delivers quantitative amplification curves within 20 minutes using loop-mediated isothermal amplification (LAMP) or recombinase polymerase amplification (RPA), detected via dual-channel fluorescence (FAM/SYBR Green)
• Robust thermal control architecture: Peltier-based heating with ±0.5 °C accuracy and uniformity across all 16 reaction wells, validated per ISO 13485 calibration protocols
• Embedded GLP-compliant data management: timestamped run logs, user authentication, audit trail export (CSV/PDF), and optional 21 CFR Part 11–ready firmware upgrade

Sample Compatibility & Compliance

The system accepts liquid-collected bioaerosol samples in SL-100-C0V1D (100-test kit) or SL-500-C0V1D (500-test kit) preservation media. Compatible with lyophilized, extraction-free amplification reagents LC-N-100 and LC-N-500—formulated for direct use with unprocessed eluates. All consumables are manufactured under ISO 13485 quality management systems and certified for CE IVD marking. The HSP200 meets national standards for portable bioaerosol samplers (GB/T 39990–2021); the ICT-208-16-YR complies with IEC 61010-1 for electrical safety and IEC 61326-1 for electromagnetic compatibility. No BSL-3 facility or RNA extraction step is required—enabling deployment in CLIA-waived settings and mobile health units.

Software & Data Management

The ICT-208-16-YR runs embedded Linux-based firmware with a 10.4″ capacitive touchscreen interface. Real-time amplification curves are rendered using adaptive baseline correction and threshold cycle (Ct) algorithms. Data export supports USB mass storage and network transfer (via RJ45 Ethernet or Wi-Fi) in CSV format, including raw fluorescence intensity, Ct values, positivity calls, operator ID, and environmental metadata (temperature, humidity if externally logged). Audit trails record every instrument interaction—including parameter changes, calibration events, and result exports—with immutable timestamps. Optional cloud synchronization enables centralized epidemiological dashboard integration for multi-site monitoring programs.

Applications

• Public health surveillance: longitudinal monitoring of airborne SARS-CoV-2 load in indoor environments to inform ventilation policy and outbreak response
• Occupational hygiene assessment: evaluation of HVAC filtration efficacy and exposure risk in high-density workplaces
• Food processing and aquaculture: detection of zoonotic pathogens (e.g., influenza A, WSSV) in production airspaces
• Veterinary diagnostics: rapid screening for canine parvovirus or feline calicivirus in animal shelters and clinics
• Plant pathology research: airborne detection of fungal spores (e.g., Puccinia, Fusarium) in greenhouse and nursery settings
• Academic and field research: method validation studies supporting WHO-recommended environmental RNA surveillance frameworks

FAQ

Does this system detect only SARS-CoV-2, or can it be adapted for other respiratory viruses?
Yes—the ICT-208-16-YR platform is assay-agnostic. With validated primer/probe sets and compatible LC-N series reagents, it supports detection of influenza A/B, RSV, hMPV, and endemic human coronaviruses (e.g., OC43, 229E).
Is nucleic acid extraction mandatory prior to analysis?
No. The LC-N series reagents are optimized for direct amplification from SL-preserved samples, eliminating centrifugation, column purification, or magnetic bead steps.
What is the limit of detection (LoD) for SARS-CoV-2 in air using this system?
Under controlled chamber testing at 200 L/min flow for 5 minutes (1,000 L total), LoD is 50–100 GE/m³ when using WHO-recommended N-gene LAMP assays—consistent with published environmental RNA surveillance benchmarks.
Can the HSP200 be mounted on autonomous platforms?
Yes. Its compact form factor (195 × 120 × 85 mm), low power draw (<5 W), and standard 1/4″-20 tripod thread (TRIPOD accessory included) support integration with ground robots, UAV gimbals, or fixed-position ceiling mounts.
Are software updates and calibration services available internationally?
Firmware updates are distributed quarterly via secure HTTPS portal; on-site or remote calibration support is provided through Zequan’s global distributor network, with traceable NIST-traceable temperature verification kits supplied with each instrument shipment.