ZWY-B3222 Dual-Layer High-Capacity Orbital Shaker

Overview

The ZWY-B3222 Dual-Layer High-Capacity Orbital Shaker is an industrial-grade, open-platform laboratory shaker engineered for high-throughput biological cultivation and biochemical reaction support under controlled ambient conditions. Designed for integration into centralized temperature-controlled environments—such as walk-in incubator rooms or dedicated fermentation suites—the unit employs a robust eccentric drive mechanism to deliver precise, low-shear orbital motion (Φ50 mm orbit) across two independently accessible stainless-steel trays. Its operation conforms to fundamental principles of orbital agitation: uniform fluid mixing via circular tray displacement, minimizing vortex formation and cell shear stress while maximizing oxygen transfer efficiency in suspension cultures. Unlike enclosed incubator shakers, the ZWY-B3222 operates without integrated temperature control; it is explicitly intended for use within externally regulated thermal environments where spatial efficiency and mechanical reliability are prioritized over on-board climate management.

Key Features

• PID microprocessor-based environmental scan controller ensures stable speed regulation across the full 30–300 rpm range, with ±1 rpm repeatability under variable load conditions.
• Backlit LCD interface displays real-time speed, elapsed time, remaining timer duration, and system status—enabling unambiguous parameter verification during extended runs (up to 500 h).
• Parameter encryption function prevents unauthorized modification of operational settings—a critical safeguard in GLP-compliant labs and shared-core facilities.
• Soft-start acceleration algorithm gradually ramps motor torque to target speed, eliminating abrupt mechanical shock and protecting delicate culture vessels and sensitive bioreactor inocula.
• Brushless AC induction motor delivers maintenance-free operation, consistent torque output across speed range, and extended service life exceeding 20,000 hours under continuous duty cycles.
• Auto-recovery logic restores pre-interruption settings—including speed, timer value, and active state—following transient power loss, supporting uninterrupted long-duration fermentation protocols.
• Structural integrity derived from precision-machined cast iron base and die-cast aluminum frame components, finished with electrostatic epoxy powder coating for corrosion resistance and cleanroom-compatible surface hygiene.

Sample Compatibility & Compliance

The ZWY-B3222 accommodates standard microbiological and bioprocessing vessels—including Erlenmeyer flasks (50–5000 mL), baffled bottles, multi-well plates (with optional adapter frames), and custom bioreactor sleeves—across its dual 920 × 640 mm trays. Its open architecture supports rapid tray access, visual inspection, and manual sampling without system shutdown. While the device itself does not carry CE, UL, or FDA 510(k) certification, its electrical design complies with IEC 61010-1:2010 safety standards for laboratory equipment. When deployed within validated environmental enclosures (e.g., ISO Class 7 cleanrooms or GMP-grade incubation chambers), the shaker supports workflows aligned with USP , ISO 13408-1, and ASTM E2500-23 guidance for equipment qualification in biopharmaceutical process development.

Software & Data Management

The ZWY-B3222 operates as a standalone hardware platform with no embedded data logging or network connectivity. All operational parameters are retained in non-volatile memory and persist through power cycles. For laboratories requiring electronic record retention, third-party USB data loggers or PLC-integrated SCADA systems may be interfaced via analog 0–10 V or 4–20 mA speed output signals (available upon request with optional I/O module). The unit’s parameter memory and encryption features facilitate audit-ready configuration control—supporting 21 CFR Part 11 compliance when paired with validated external software platforms that enforce user authentication, change tracking, and electronic signature workflows.

Applications

This shaker is routinely deployed in upstream bioprocess development for aerobic microbial cultivation (e.g., E. coli, Bacillus subtilis, yeast), recombinant protein expression, plasmid amplification, and enzyme production. It serves as a scalable bridge between shake-flask screening and benchtop bioreactor studies—particularly where parallelization of strain variants or media formulations is required. Additional validated applications include hybridoma cell expansion, antibody fragment purification pre-steps, diagnostic reagent synthesis, environmental microbiology enrichment cultures, and food safety pathogen propagation per AOAC and ISO 6579 protocols. Its natural convection compatibility enables seamless integration into large-volume incubator rooms used in QC/QA labs across pharmaceutical, academic, and contract research organizations.

FAQ

Is the ZWY-B3222 suitable for use inside a CO₂ incubator?
No. The unit lacks gas-tight sealing and is rated only for ambient air environments (5–50 °C); it must be installed outside CO₂ chambers and operated within temperature-stabilized rooms.
Can the two trays operate at different speeds?
No. Both trays are mechanically coupled and run synchronously at a single user-defined speed.
What is the maximum flask loading capacity per tray?
Each tray supports up to 24 × 2 L flasks or 48 × 500 mL flasks using standard universal clamps—subject to center-of-gravity balance and total mass limits (max 45 kg per tray).
Does the unit include vibration isolation feet?
Yes. Adjustable anti-vibration rubber mounts are factory-installed to minimize structural transmission and meet ISO 2631-1 hand-arm vibration exposure thresholds.
Is calibration documentation provided with shipment?
A factory-verified speed calibration certificate (traceable to NIM, China) is included; users may perform periodic verification using a handheld tachometer per ISO/IEC 17025 procedures.