Beijing Liuyi DYCZ-20B DNA Sequencing Electrophoresis Apparatus

Overview

The Beijing Liuyi DYCZ-20B DNA Sequencing Electrophoresis Apparatus is a vertical slab gel electrophoresis system engineered for high-resolution separation of DNA fragments in sequencing applications, including Sanger-based dideoxy sequencing, differential display PCR, DNA fingerprinting, and single-strand conformation polymorphism (SSCP) analysis. It operates on the principle of electrophoretic mobility under an applied DC electric field, where nucleic acid fragments migrate through a polyacrylamide or agarose matrix at rates inversely proportional to their molecular weight and conformation. Designed for compatibility with standard vertical electrophoresis power supplies—including the DYY-10C, DYY-12, DYY-12C, DYY-12D, and DYY-15D series—the DYCZ-20B supports denaturing and non-denaturing conditions, enabling reproducible band resolution down to single-base differences in optimized runs. Its robust acrylic body and precision-machined aluminum heat-dissipating base ensure thermal uniformity across the gel surface during extended high-voltage runs, minimizing band distortion caused by localized Joule heating.

Key Features

• Vertical slab gel configuration optimized for DNA sequencing gels up to 480 mm × 170 mm, accommodating standard sequencing formats and multi-lane analytical setups.
• Integrated buffer drainage port facilitating rapid, spill-free buffer replacement and system cleaning between runs—critical for minimizing cross-contamination in high-throughput labs.
• Dual comb support: includes two 32-tooth shark-tooth combs and two 26-tooth castle-tooth combs (0.4 mm thickness), enabling flexible sample loading patterns for both high-density sequencing ladders and comparative genotyping assays.
• Thermally balanced design with passive convection-enhanced baseplate and low-thermal-mass glass plate assembly, maintaining stable temperature gradients even during prolonged electrophoresis at 150–200 V.
• Modular gel-casting system featuring reusable concave and flat glass plates (2 each), silicone sealing strips, adjustable clamping frames, and a calibrated gel-pouring bottle for consistent, bubble-free polymerization.
• Ergonomic handling: lightweight (9 kg) yet rigid frame with integrated cable management and recessed electrode terminals compatible with standard banana-jack power leads.

Sample Compatibility & Compliance

The DYCZ-20B is validated for use with standard polyacrylamide gels (e.g., 4–8% T, 2.6–5% C formulations) and urea-denaturing matrices commonly employed in manual and semi-automated DNA sequencing workflows. It supports both radioactive (³²P) and non-radioactive detection methods (e.g., silver staining, SYBR Gold, ethidium bromide). While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 out-of-the-box, the apparatus conforms to general laboratory safety standards for Class II electrophoresis equipment (IEC 61010-1). Its mechanical design allows full traceability in GLP-compliant environments when paired with documented SOPs for gel preparation, voltage ramping, and run duration control. Buffer volume (750 mL) meets ASTM D5127-18 recommendations for vertical electrophoresis systems operating above 100 V.

Software & Data Management

As a standalone electrophoresis apparatus, the DYCZ-20B does not incorporate embedded firmware or digital controls. All operational parameters—including voltage, current, run time, and cooling—are managed externally via compatible power supplies (e.g., DYY-series units with digital displays and programmable timers). For data integrity in regulated settings, users are advised to integrate the system into existing LIMS or ELN platforms using manual entry protocols or barcode-scanned run logs. When coupled with third-party gel documentation systems (e.g., UV transilluminators with CCD imaging), raw band images can be exported in TIFF or PNG format for downstream analysis using open-source tools such as ImageJ/Fiji or commercial packages compliant with 21 CFR Part 11 audit trail requirements.

Applications

• DNA sequencing ladder resolution (e.g., M13mp18 RF sequencing reactions)
• SSCP-based mutation screening in clinical research and pharmacogenomics
• Microsatellite and AFLP fragment analysis for population genetics studies
• Differential display profiling of cDNA expression variants
• Verification of CRISPR-Cas9 editing outcomes via fragment size shift detection
• Quality control of synthetic oligonucleotides and PCR amplicons prior to cloning or NGS library prep

FAQ

Is the DYCZ-20B compatible with automated gel loaders or robotic pipetting systems?
No—it is a manually operated electrophoresis chamber designed for benchtop use; integration with automation requires custom mounting fixtures and third-party liquid handling validation.
What power supply models are officially recommended for optimal performance?
The DYY-12D and DYY-15D power supplies are factory-validated for use with the DYCZ-20B, offering constant voltage/current modes and over-current protection suitable for sequencing-grade runs.
Can this unit be used for protein electrophoresis?
While technically feasible for low-percentage SDS-PAGE gels, the DYCZ-20B lacks built-in cooling and is not optimized for high-molecular-weight protein separation; dedicated protein electrophoresis systems are recommended for reproducible results.
Are replacement parts such as glass plates and combs available separately?
Yes—Beijing Liuyi provides OEM part numbers (e.g., 142-2021, 141-2012) for individual components, ensuring long-term serviceability and compliance with original design specifications.
Does the apparatus meet CE or UL safety certification requirements?
It complies with GB/T 14710-2009 (Chinese national standard for environmental adaptability of medical electrical equipment) but is not CE-marked or UL-listed; users in EU/US markets should verify local regulatory acceptance prior to installation.