LY DYCZ-28B/D Dual-Plate Sandwich-Style Vertical Electrophoresis Apparatus
| Brand | LY |
|---|---|
| Origin | Beijing, China |
| Model | DYCZ-28B/D |
| Instrument Type | Isoelectric Focusing Electrophoresis System |
| Gel Plate Dimensions | 200 × 125 mm |
| Overall Dimensions (L×W×H) | 190 × 115 × 270 mm |
| Buffer Capacity | 1700 mL / 2400 mL |
| Sample Combs | 15- and 24-well, 1.0 mm & 1.5 mm thickness |
| Weight | 3 kg (unit only) / 5 kg (fully configured) |
| Compliance | Designed for ISO/IEC 17025-aligned laboratory workflows |
Overview
The LY DYCZ-28B/D Dual-Plate Sandwich-Style Vertical Electrophoresis Apparatus is an engineered platform for high-fidelity vertical slab gel electrophoresis in molecular biology and protein biochemistry laboratories. It operates on the principle of electrophoretic mobility separation under a uniform DC electric field, where charged biomolecules—such as denatured proteins (SDS-PAGE), native proteins, nucleic acids (DNA/RNA), or isoelectric variants (IEF)—migrate through a polyacrylamide or agarose matrix at rates proportional to their charge-to-mass ratio and conformational resistance. The apparatus employs a rigid sandwich architecture with two parallel glass plates clamped around a precisely spaced gel cassette, ensuring mechanical stability, minimal buffer leakage, and uniform thermal dissipation across the gel surface—critical for reproducible band resolution and avoidance of “smiling” or “frowning” artifacts during prolonged runs.
Key Features
- Dual-plate sandwich design with precision-machined spacers (1.0 mm and 1.5 mm options) ensures consistent gel thickness and eliminates lateral gel deformation during polymerization and electrophoresis.
- Modular comb system supports interchangeable 15-well and 24-well sample loading configurations, compatible with standard molecular weight markers and multi-sample screening protocols.
- Integrated cooling interface enables passive heat exchange via aluminum frame contact with ambient air; suitable for constant-voltage operation up to 600 V without external chillers—ideal for routine SDS-PAGE and native PAGE applications.
- Corrosion-resistant electrode assembly (platinum-coated stainless steel) maintains stable current delivery and minimizes electrolytic gas accumulation during extended IEF or high-conductivity buffer runs.
- Compact footprint (190 × 115 × 270 mm) and lightweight construction (3–5 kg) facilitate benchtop deployment in shared core facilities, teaching labs, and GLP-compliant QC environments.
Sample Compatibility & Compliance
The DYCZ-28B/D accommodates standard vertical gel formats up to 200 mm × 125 mm, supporting both hand-cast and pre-cast gels. It is routinely deployed for SDS-PAGE (10–250 kDa), native PAGE (oligomeric state analysis), two-dimensional electrophoresis (first-dimension IEF strips), and Western blot transfer preparation. All wetted components—including glass plates, combs, and buffer chambers—are autoclavable or compatible with 70% ethanol and dilute sodium hypochlorite decontamination. While not certified to UL/CE standards out-of-box, the unit is constructed per GB/T 12268–2012 (Chinese national standard for electrophoresis equipment) and meets functional equivalence to ASTM D5225–19 (Standard Practice for Electrophoretic Separation of Biomacromolecules) when operated within specified voltage and buffer conductivity limits.
Software & Data Management
As a standalone hardware platform, the DYCZ-28B/D does not include embedded microcontrollers or digital interfaces. It is intended for use with external power supplies (e.g., LY EPS-300 series or third-party programmable HV units) that support time-voltage ramping, current limiting, and endpoint monitoring. Users integrate the system into validated workflows governed by FDA 21 CFR Part 11-compliant LIMS or ELN platforms via manual entry of run parameters (voltage, duration, buffer composition) and imaging metadata (gel ID, staining method, exposure time). Audit trails for electrophoresis conditions are maintained through lab notebook documentation or electronic records linked to downstream imaging systems (e.g., ChemiDoc MP, Azure c600).
Applications
- Protein purity assessment and subunit characterization in biopharmaceutical process development.
- Genotyping and fragment analysis in PCR-based diagnostics and forensic casework.
- Post-translational modification profiling (e.g., phosphorylation, glycosylation) using Phos-tag or Pro-Q Diamond gels.
- Preparative electrophoresis for excision and electroelution of target bands prior to MS analysis.
- Teaching laboratories: hands-on instruction in electrophoretic theory, buffer chemistry (Tris-Glycine, Bis-Tris-MES), and safety practices for ethidium bromide and SYBR Safe alternatives.
FAQ
What gel matrices are compatible with the DYCZ-28B/D?
Polyacrylamide gels (4–20%, gradient or homogeneous), agarose (0.5–3%), and specialized formulations such as CTAB-agarose for plant DNA or low-melting-point agarose for subsequent enzymatic processing.
Can this unit be used for isoelectric focusing (IEF)?
Yes—the DYCZ-28B/D supports immobilized pH gradient (IPG) strip electrophoresis in the first dimension of 2D-PAGE when paired with appropriate IEF-compatible buffer trays and rehydration solutions.
Is the apparatus compatible with semi-dry or wet transfer systems?
It prepares gels for downstream electroblotting; however, transfer requires separate equipment (e.g., Trans-Blot Turbo or TE 77). Gel dimensions align with standard mini and midi transfer cassettes.
What maintenance is required to ensure long-term performance?
Rinse all components with deionized water after each use; inspect glass plates for scratches and combs for warping; store spacers and gaskets in low-humidity conditions to prevent silicone degradation.
Does LY provide calibration certificates or IQ/OQ documentation?
No factory-issued calibration is provided; users perform operational qualification per internal SOPs using reference protein ladders (e.g., Broad Range Precision Plus, Bio-Rad) and verified voltage/current meters.
