Beijing Liuyi WD-9403F Multi-Function UV Transilluminator (Model 130-0360)

Overview

The Beijing Liuyi WD-9403F Multi-Function UV Transilluminator (Model 130-0360) is a benchtop, darkroom-integrated analytical instrument engineered for nucleic acid and protein visualization in molecular biology laboratories. It operates on the principle of ultraviolet-induced fluorescence—where ethidium bromide, SYBR® dyes, or other nucleic acid intercalators absorb UV photons at specific wavelengths (254 nm, 302 nm, or 365 nm) and re-emit visible light, enabling real-time detection of DNA/RNA bands in agarose or polyacrylamide gels. Unlike open-frame UV boxes requiring dedicated darkrooms, the WD-9403F features a fully enclosed, light-tight cabinet with front-access viewing ports and dual-side cutout apertures—permitting safe, ergonomic gel excision under controlled UV exposure. Its modular lamp architecture supports simultaneous or independent operation of transmissive (302 nm) and reflective (254/365 nm) UV sources, along with interchangeable white-light transillumination—making it suitable for Coomassie- or silver-stained protein gels, TLC plates, and UV-active compound screening.

Key Features

• Triple-wavelength UV capability: 302 nm (optimized for ethidium bromide-stained nucleic acids), 254 nm (high-energy reflection for UV-absorbing compounds), and 365 nm (long-wave reflection for low-photodamage applications and safer visualization)
• Interchangeable drawer-style lamp modules: UV transilluminator unit and white-light box slide in/out without tools—minimizing cross-contamination and simplifying maintenance
• Integrated safety design: Fully shielded cabinet with UV-blocking acrylic lid, automatic lamp cutoff upon lid opening, and internal LED cabin lighting for sample handling without UV exposure
• Precision irradiance output: Each UV channel delivers ≥10 µW/cm² at the gel surface—ensuring consistent fluorescence excitation across the full 200 × 200 mm active area
• Ergonomic workflow support: Dual lateral access openings allow direct scalpel-based band excision; optional camera mount interface (M52 thread) enables standardized documentation under reproducible illumination conditions

Sample Compatibility & Compliance

The WD-9403F accommodates standard electrophoresis formats including mini-gels (up to 200 × 200 mm), TLC aluminum/silica plates, and thin-layer chromatography sheets. Its 302 nm transilluminator meets common laboratory requirements for post-electrophoretic DNA imaging per ISO 15190:2020 (Laboratory safety—General requirements) and aligns with institutional biosafety protocols for UV exposure minimization. While not certified to IEC 61000-4 or FDA 21 CFR Part 11 out-of-the-box, the system’s mechanical interlock, labeled wavelength identifiers, and stable irradiance output support GLP-compliant documentation workflows when paired with validated imaging systems and audit-trail-capable software (e.g., ImageLab™ or Quantity One®). All UV lamps conform to GB/T 20145–2006 (China national standard for UV lamp safety and performance).

Software & Data Management

The WD-9403F is a hardware-only platform—no embedded firmware or proprietary software is included. It functions as a calibrated illumination source compatible with third-party digital imaging systems. When integrated with scientific-grade CCD or CMOS cameras (e.g., Canon EOS R-series or Teledyne Photometrics monochrome units), users may leverage open-standard image acquisition tools such as Fiji/ImageJ, GelAnalyzer, or commercial packages like Bio-Rad Quantity One®. Optional M52-thread camera adapters and UV-blocking filter kits (e.g., LP420 long-pass for 302 nm imaging) ensure spectral fidelity and reduce background noise. For regulated environments, laboratories are advised to establish instrument-specific SOPs—including daily irradiance verification using NIST-traceable UV radiometers—and maintain calibration logs per ISO/IEC 17025:2017 clause 6.5.

Applications

• Visualization and documentation of DNA/RNA bands in agarose and PAGE gels stained with EtBr, SYBR® Gold, GelRed®, or similar fluorophores
• UV reflectance imaging of oligonucleotide synthesis monitoring, plasmid restriction digests, and PCR product verification
• Protein gel analysis via white-light transillumination (Coomassie, silver stain) or UV absorption (tryptophan/tyrosine native gels)
• TLC plate development tracking for organic synthesis, natural product isolation, and pharmaceutical QC
• UV-curable resin inspection and photopolymer characterization in materials science labs

FAQ

Is the WD-9403F compliant with international electrical safety standards?
It complies with China’s GB 4793.1–2007 (equivalent to IEC 61010-1:2010) for laboratory electrical equipment. CE or UL certification is not provided; end-users must verify local regulatory acceptance prior to installation.
Can this unit be used for UV crosslinking?
No. The WD-9403F is designed solely for visualization—not nucleic acid crosslinking. Crosslinking requires higher irradiance (>100 mJ/cm²) and dedicated 254 nm crosslinkers with dosimetry control.
What is the expected lifetime of the UV lamps?
Typical rated life is 1,000–1,500 hours under intermittent use. Output degradation accelerates with frequent thermal cycling; replacement lamps (part nos. 430-3350, 430-3147) are available through authorized Beijing Liuyi distributors.
Does the system include UV exposure timers or intensity modulation?
No. Intensity is fixed per lamp module; exposure duration must be controlled manually or via external camera shutter timing. No built-in timer or dimming circuitry is integrated.
Are there service or calibration options available outside mainland China?
Beijing Liuyi provides technical documentation and spare parts lists globally. Local authorized service partners (e.g., VWR International, Thomas Scientific) may offer field support depending on regional distribution agreements—but no factory-certified recalibration service is offered internationally.