CZGY HY-45A Refrigerated Air-Bath Orbital Shaker

Overview

The CZGY HY-45A Refrigerated Air-Bath Orbital Shaker integrates precise temperature control with consistent orbital agitation in a single compact platform. Engineered for life science laboratories requiring simultaneous thermal regulation and mechanical mixing, it operates on the principle of forced-air convection heating and vapor-compression refrigeration—enabling stable, uniform temperature maintenance across the entire chamber while sustaining reproducible oscillatory motion. Unlike water-bath shakers, the air-bath design eliminates fluid contamination risks, simplifies maintenance, and supports rapid temperature transitions between setpoints. Its brushless DC motor ensures low-vibration, long-life operation without carbon dust accumulation—a critical requirement for extended cell culture, microbial fermentation, and enzyme kinetics experiments. The unit is designed for continuous duty cycles under controlled environmental conditions, meeting foundational operational expectations for GLP-aligned research environments.

Key Features

• Refrigerated air-bath system with dual-mode thermal control: active cooling (via sealed R134a compressor) and resistive heating (500 W), enabling precise setpoint stability from 5 °C to 50 °C
• Fuzzy PID temperature controller with auto-tuning function (Atu mode), adjustable hysteresis (Hyc), and sensor offset calibration (Sc parameter) for traceable accuracy verification
• Orbital shaking mechanism with fixed 30 mm amplitude and digitally regulated speed range of 40–300 rpm (±0.5 rpm repeatability)
• Single large-format stainless-steel platform (470 × 400 mm) with reinforced polymer-coated surface and standardized flask-mounting fixtures
• Dual independent timers: one for temperature hold duration, another for oscillation runtime—both programmable up to 9999 minutes (166.6 hours)
• Compressor protection logic: configurable restart delay (Alt parameter, default 140 s) prevents high-pressure start-up damage
• Intuitive 4-digit LED interface with dedicated SET, △/▽, H/M, RUN/STOP keys; no touchscreen or proprietary software dependency

Sample Compatibility & Compliance

The HY-45A accommodates standard laboratory glassware and plastic vessels—including Erlenmeyer flasks (150–1000 mL), culture tubes, and multi-well plates—secured via universal clamps or optional custom adapters. Its stainless-steel interior chamber and thick acrylic observation window provide corrosion resistance and optical clarity without compromising structural integrity. While not certified to ISO/IEC 17025 or FDA 21 CFR Part 11 out-of-the-box, the instrument’s deterministic control architecture, non-volatile parameter storage, and audit-ready timer logs support internal validation protocols required for QC/QA workflows in pharmaceutical, academic, and biotech settings. Temperature uniformity (±1 °C) and accuracy (±0.5 °C) are verified per ASTM E2234–22 guidelines for laboratory thermal equipment.

Software & Data Management

The HY-45A operates as a standalone embedded system with no external PC dependency. All operational parameters—including setpoints, timers, PID coefficients (P, I, D), compressor thresholds (AFL/AFH), and calibration offsets—are stored in non-volatile memory and retain values after power interruption. The controller supports manual calibration traceability through Sc adjustment and provides visual status indicators (green RUN / red STOP LEDs). Though it lacks USB/Ethernet connectivity or cloud integration, its deterministic behavior, repeatable ramp-and-hold profiles, and absence of firmware updates make it suitable for validated environments where change control documentation must accompany any configuration modification.

Applications

• Microbial growth studies requiring sub-ambient incubation (e.g., psychrophilic strain cultivation at 10–15 °C)
• Recombinant protein expression in E. coli or yeast under controlled induction temperatures (e.g., 18 °C for soluble folding)
• Enzyme activity assays involving temperature-sensitive kinetics (e.g., restriction digests, ligations, phosphorylation reactions)
• Suspension cell culture maintenance during transfection or passaging protocols
• Antibiotic susceptibility testing using broth microdilution methods per CLSI M07-A11
• Environmental sample processing—including soil extract agitation and wastewater microbiome enrichment—at defined thermal setpoints

FAQ

Does the HY-45A support programming multiple temperature-ramp steps?
No. It implements single-setpoint temperature control with fixed hold duration; multi-step thermal profiling requires external scheduling or manual intervention.
Can the shaker operate without refrigeration below ambient temperature?
Yes—compressor activation is automatically triggered only when the setpoint falls below the AFH threshold (default 35 °C); operation at 25–30 °C uses heating-only or passive stabilization.
Is the platform compatible with magnetic stir bars or heated blocks?
No. The unit is optimized for orbital agitation of liquid-filled vessels; integration of auxiliary heating or stirring accessories is not supported by design.
What maintenance intervals are recommended for the refrigeration system?
Annual visual inspection of condenser coils and fan operation is advised; compressor oil and refrigerant charge require service only if performance degradation exceeds ±1.5 °C deviation over three consecutive validation cycles.
Are replacement fixtures available for non-standard vessel geometries?
Yes—CZGY offers OEM-compatible custom clamp kits upon request, subject to dimensional specifications and minimum order quantities.