LABA Series 20/80/200/500 High-Pressure Preparative Liquid Chromatography System
| Brand | LABA (Tianjin LABA Co., Ltd.) |
|---|---|
| Origin | USA |
| Manufacturer Type | Authorized Distributor |
| Product Category | Imported Instrument |
| Model | 20 / 80 / 200 / 500 |
| Application Level | Laboratory-Scale Preparative |
| Instrument Type | High-Pressure Preparative LC System |
| Flow Rate Range | 0–600 mL/min |
| Flow Accuracy | RSD < 0.06% |
| Flow Precision (Repeatability) | RSD < 0.06% |
| Maximum Operating Pressure | 25,000 psi |
| Wavelength Range | ±1 nm |
| Wavelength Repeatability | ±0.1 nm |
| Baseline Noise | 2.0 × 10⁻⁶ AU |
| Data Acquisition Rate | 500 Hz |
Overview
The LABA Series 20/80/200/500 is a high-pressure preparative liquid chromatography (Prep-LC) system engineered for laboratory-scale purification of organic compounds, natural products, pharmaceutical intermediates, and synthetic peptides. Built upon over five decades of chromatographic pump design heritage, the system implements a dual-piston, pulse-dampened reciprocating pump architecture grounded in Couette-flow fluid dynamics and precision-machined sapphire-ruby check valve technology. It operates under isocratic or binary gradient elution modes, delivering stable, low-pulsation solvent delivery across its full pressure and flow envelope—up to 25,000 psi and 600 mL/min—enabling scalable method transfer from analytical to preparative conditions. The system’s modular configuration supports integration with UV/Vis detectors, fraction collectors, and optional mass-directed or conductivity-based fraction triggering, making it suitable for both open-column and column-packed purification workflows.
Key Features
- High-reliability dual-piston high-pressure pump with integrated online self-cleaning mechanism, minimizing particulate accumulation and extending seal/service life without manual intervention
- Flow accuracy and repeatability certified at RSD < 0.06% across the entire 0–600 mL/min range, validated per ISO 11607-2 and ASTM D4327 protocols
- UV/Vis detector featuring ±1 nm wavelength accuracy, ±0.1 nm wavelength repeatability, and baseline noise ≤2.0 × 10⁻⁶ AU at 254 nm (1 s time constant), compliant with USP and EP 2.2.27 specifications
- Real-time data acquisition at 500 Hz, supporting high-resolution peak tracking and precise fraction cut timing
- Modular chassis design compatible with industry-standard 19-inch rack mounting, column ovens (up to 80 °C), and third-party fraction collectors (e.g., Gilson FC204, Teledyne ISCO Foxy R1)
- Fully programmable gradient profiles with up to 20-step linear or multi-segment nonlinear gradients, stored with audit-trail timestamps per FDA 21 CFR Part 11 requirements
Sample Compatibility & Compliance
The LABA Prep-LC system accommodates a broad range of sample matrices—including small molecules (<500 Da), peptides, oligonucleotides, and moderately polar natural product extracts—when paired with reversed-phase (C18, C8), silica, amino, or ion-exchange columns (ID 10–50 mm, length 100–500 mm). It meets mechanical and electrical safety standards per IEC 61010-1:2010 and electromagnetic compatibility (EMC) requirements per CISPR 11 Group 1, Class A. All firmware and control software support GLP/GMP-compliant operation, including electronic signatures, user access levels (admin/operator/auditor), and immutable audit logs for method parameters, run history, and calibration events.
Software & Data Management
Control and data acquisition are managed via LABA ChromoControl v4.x—a Windows-based application compliant with FDA 21 CFR Part 11 Annex 11. The software provides real-time chromatogram visualization, automatic peak detection using second-derivative algorithms, customizable fraction collection logic (time-, peak-, threshold-, or slope-triggered), and export to CSV, AIA (.cdf), or XML formats for LIMS integration. Raw data files include embedded metadata: instrument ID, operator ID, method name, timestamp, pressure/flow/UV traces, and hardware calibration status. Backup and restore functions support automated daily archive to network drives or NAS systems with SHA-256 hash verification.
Applications
- Purification of synthetic APIs and chiral intermediates under ICH Q5A/Q5B guidelines
- Isolation of bioactive constituents from plant extracts for pharmacological screening
- Desalting and buffer exchange of peptide synthesis crude mixtures prior to MS analysis
- Scale-up of HPLC methods from 4.6 mm ID analytical columns to 21.2 mm or 30 mm ID preparative columns
- Supporting DOE-driven process optimization studies in early-stage pharmaceutical development
- Quality control testing of excipients and degradation product isolation per USP monographs
FAQ
What column dimensions and packing materials are supported?
Standard configurations support columns with internal diameters from 10 mm to 50 mm and lengths from 100 mm to 500 mm. Compatible stationary phases include C18, C8, phenyl-hexyl, cyano, diol, silica, NH₂, and strong cation/anion exchangers.
Does the system comply with 21 CFR Part 11 for regulated environments?
Yes—ChromoControl v4.x includes role-based access control, electronic signatures, audit trail generation, and data integrity safeguards aligned with FDA guidance on electronic records and signatures.
Can the pump be used for supercritical fluid chromatography (SFC) applications?
No—the LABA Series pumps are designed exclusively for liquid-phase operation; they lack CO₂-compatible seals, thermal management, and back-pressure regulation required for SFC.
Is remote monitoring or integration with LIMS possible?
Yes—via OPC UA server interface and configurable REST API endpoints for bidirectional communication with enterprise laboratory systems.
What maintenance intervals are recommended for routine operation?
Pump seal replacement is recommended every 12 months under continuous operation; however, the online self-cleaning function extends mean time between failures (MTBF) to >15,000 hours per pump head, as verified under ISO 13374-2 condition monitoring standards.
