Wufeng LC-100 Third-Generation High-Performance Liquid Chromatograph
| Brand | Wufeng |
|---|---|
| Origin | Shanghai, China |
| Model | LC-100 |
| Instrument Type | Conventional HPLC System |
| Flow Rate Range | 0.001–9.999 mL/min |
| Maximum Pressure | 42 MPa |
| Sample Tray Capacity | 108 positions (2 mL vials) / 70 positions (4 mL vials) / 30 positions (10 mL vials) |
| Injection Volume Range | 0.1–200 µL (with interchangeable loop) |
| Column Oven Temperature Range | (Ambient + 5)°C to 80°C |
| UV-Vis Wavelength Range | 190–700 nm (standard) / 190–900 nm (optional DAD) |
| Data Acquisition Rate | up to 100 Hz (DAD) |
Overview
The Wufeng LC-100 is a third-generation high-performance liquid chromatograph engineered for precision, robustness, and method flexibility in routine and research-grade analytical laboratories. Built upon two decades of focused development in HPLC system architecture, the LC-100 implements classical reversed-phase, normal-phase, ion-exchange, and size-exclusion chromatography using isocratic or gradient elution modes. Its modular design integrates independently optimized subsystems—including dual-piston reciprocating pumps, a temperature-stabilized autosampler with configurable tray geometry, a thermally isolated column oven, and a choice of detection modules—each conforming to fundamental chromatographic principles of retention time reproducibility, peak shape fidelity, and baseline stability. The system operates under laminar flow conditions compliant with Poiseuille’s law, ensuring predictable pressure-flow relationships across its full 0.001–9.999 mL/min range and supporting method scalability from analytical to semi-preparative applications.
Key Features
- Modular gradient capability: Configurable as binary, quaternary, or isocratic solvent management system; supports up to eight solvents with optional integrated vacuum degasser.
- Precision pumping architecture: Dual-plunger, pulse-dampened reciprocating pumps delivering ≤0.1% RSD flow precision at 1 mL/min and ≤0.2% RSD over 0.01–10 mL/min range.
- Autosampler with multi-format compatibility: Programmable tray layout accommodating 108 × 2 mL vials, 70 × 4 mL vials, or 30 × 10 mL vials; injection volume adjustable from 0.1 to 200 µL via user-replaceable fixed loops.
- Integrated column compartment: Thermally uniform oven with Peltier-based heating/cooling, maintaining ±0.2°C stability across 5–80°C operating range; designed with co-located solvent reservoir tray to minimize thermal gradient effects on mobile phase composition.
- Detection versatility: Standard UV-Vis detector (190–700 nm), optional DAD (190–900 nm, 100 Hz acquisition), fluorescence, RID, ELSD, and other third-party compatible detectors.
Sample Compatibility & Compliance
The LC-100 accommodates standard 4.6 mm i.d. analytical columns (up to 250 mm length) and 10 mm i.d. semi-preparative columns (up to 150 mm), supporting common stationary phases including C18, C8, phenyl-hexyl, HILIC, and silica. It complies with ISO 9001 manufacturing protocols and meets mechanical safety requirements per IEC 61010-1. Software operation supports audit trail generation, electronic signatures, and data integrity controls aligned with FDA 21 CFR Part 11 when deployed with validated firmware and networked authentication. Method parameters are exportable in ASTM E1992-compliant ASCII format for cross-platform instrument interoperability.
Software & Data Management
ChromSight™ LC Control & Analysis Suite (v3.x) provides intuitive sequence setup, real-time chromatogram visualization, peak integration with customizable baselines, and automated calibration curve generation. All raw data files (.chd) are stored in vendor-neutral HDF5 format with embedded metadata (instrument configuration, method file hash, operator ID, timestamp). The software supports GLP/GMP workflows through mandatory electronic log entries for method changes, maintenance events, and calibration verification. Data backups adhere to ISO/IEC 27001-aligned encryption standards during local or NAS-based archival.
Applications
The LC-100 serves as a primary analytical platform in pharmaceutical QC labs for assay validation, impurity profiling, and dissolution testing per USP and ICH Q2(R2); in food safety labs for pesticide residue screening (AOAC 2007.01), mycotoxin quantification (EN 14132), and vitamin analysis; and in environmental testing for PAHs, PCBs, and pharmaceutical metabolites in wastewater matrices (EPA Method 8330B). Its pressure tolerance (42 MPa) and low-flow precision enable method transfer from UHPLC systems using 2.1 mm i.d. columns without hardware modification.
FAQ
Is the LC-100 compliant with 21 CFR Part 11 for regulated environments?
Yes—when operated with ChromSight™ v3.2+ and configured with role-based access control, electronic signatures, and immutable audit trails.
Can the autosampler accommodate custom vial formats or deep-well plates?
Standard trays support ANSI/SLAS-compliant 96-well microplates (optional adapter kit required); non-standard vials require mechanical reconfiguration by certified service personnel.
What is the maximum dwell volume for the quaternary gradient module?
Typical dwell volume is 750 µL (measured per ISO 17025-accredited protocol using acetone step gradient); system optimization reduces gradient delay to ≤1.2 column volumes for 150 mm × 4.6 mm columns.
Does the DAD module support spectral library matching?
Yes—the software includes NIST-compatible spectral search engine with built-in libraries for >12,000 compounds; users may import proprietary reference spectra in JCAMP-DX format.
Is remote monitoring supported?
Instrument status, queue progress, and alarm notifications are accessible via secure HTTPS interface; full remote control requires on-premise VPN or Wufeng-certified cloud gateway deployment.
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