BUCHI Pure C-835/C-830 High-Pressure Preparative Liquid Chromatography System
| Brand | BUCHI |
|---|---|
| Origin | Switzerland |
| Manufacturer | BUCHI AG |
| Product Type | Imported Instrument |
| Model | Pure C-835 / Pure C-830 |
| Application Level | Laboratory Grade |
| Instrument Category | High-Pressure Preparative Liquid Chromatography |
| Flow Rate Range | 1–100 mL/min |
| Flow Accuracy | ±3% RSD |
| Flow Precision | ±0.5% RSD |
| Maximum Pressure | 300 bar |
| Wavelength Accuracy | ±1 nm |
| Wavelength Reproducibility | 0.4 nm |
| Baseline Noise | 5 × 10⁻⁵ AU |
| Data Acquisition Frequency | 5 Hz |
Overview
The BUCHI Pure C-835 and Pure C-830 are high-pressure preparative liquid chromatography (PLC) systems engineered for robust, scalable, and safe purification of organic and pharmaceutical compounds in laboratory-scale R&D environments. Based on classical high-performance liquid chromatography (HPLC) principles—employing isocratic or gradient elution under controlled pressure, flow, and detection—the Pure series delivers reproducible fractionation across milligram-to-hundred-gram sample loads. Designed and manufactured in Flawil, Switzerland, the systems integrate a modular architecture with integrated safety engineering, including full enclosure, real-time leak and overflow monitoring, and remote operation capability. Their compact footprint (≤0.8 m²) enables deployment in standard fume hoods or benchtop settings without compromising operational integrity or regulatory compliance.
Key Features
- High-pressure binary gradient pumping system capable of sustained operation up to 300 bar (4350 psi), supporting both reversed-phase (C18, phenyl, etc.) and normal-phase (silica, cyano, amino) separations.
- Multi-channel detection architecture accommodating simultaneous UV/VIS absorbance (with diode array scanning), evaporative light scattering (ELSD), and optional mass spectrometry interfaces—enabling orthogonal compound characterization.
- Automated ELSD module with self-optimizing gas flow and temperature control; fixed split-flow of 30 µL/min at total flow rates from 100 mL/min, ensuring consistent nebulization and signal stability.
- Hermetically sealed fraction collector equipped with RFID-enabled rack recognition, real-time solvent level sensing, and vapor concentration monitoring—preventing exposure, mis-collection, and uncontrolled solvent evaporation.
- Remote method editing and live system monitoring via Ethernet-connected PC or tablet using BUCHI’s intuitive PureControl software interface—supporting audit trails and user access control per GLP/GMP requirements.
- Integrated safety interlocks: automatic shutdown upon detected leak, waste bottle overfill, door opening during pressurized operation, or thermal excursion beyond defined thresholds.
Sample Compatibility & Compliance
The Pure C-835/C-830 accommodates a broad range of analytes—including small-molecule APIs, natural products, synthetic intermediates, peptides, and polar metabolites—across diverse mobile phase chemistries (e.g., water/acetonitrile, hexane/ethyl acetate, THF/methanol). Column compatibility spans standard 10–50 mm ID preparative columns (including BUCHI’s PrepPure and GlasPure lines with 5–15 µm spherical silica, C18, NH₂, and CN phases) as well as custom-packed cartridges. The system meets key international standards for analytical instrumentation, including ISO 9001:2015 (design and manufacturing), IEC 61010-1 (electrical safety), and supports 21 CFR Part 11-compliant data integrity workflows when configured with electronic signatures and time-stamped audit logs.
Software & Data Management
PureControl software provides full instrument control, method development, real-time chromatogram visualization, peak integration, and fraction-triggered collection logic (time-, peak-, or threshold-based). All acquisition parameters—including flow rate, gradient profile, UV wavelength setpoints, ELSD gain, and collection mapping—are stored in structured XML-based method files. Raw data (absorbance, ELSD response, pressure, temperature) are saved in vendor-neutral .csv and .chm formats for third-party processing. Audit trail functionality records all user actions, parameter changes, and system events with immutable timestamps—fully traceable for internal QA review or external regulatory inspection (e.g., FDA, EMA).
Applications
- Purification of reaction mixtures post-synthesis in medicinal chemistry and process R&D.
- Isolation of natural product fractions from plant extracts or fermentation broths.
- Desalting and buffer exchange of peptides and oligonucleotides prior to structural analysis.
- Chiral separation using dedicated chiral stationary phases (e.g., Chiralpak®, Lux®).
- Scale-up support from analytical to semi-preparative conditions using retention time and selectivity transfer algorithms embedded in PureControl.
- Method development for downstream QC release testing in accordance with USP , EP 2.2.46, and ICH Q2(R2) guidelines.
FAQ
What column formats are compatible with the Pure C-835/C-830?
Standard stainless steel or PEEK-packed columns with 10–50 mm internal diameter and 50–250 mm length; compatible with BUCHI PrepPure (stainless steel) and GlasPure (glass) series, as well as third-party columns meeting SAE J514 hydraulic fitting specifications.
Does the system support GMP-compliant data handling?
Yes—when deployed with PureControl v3.2+ and configured with role-based user accounts, electronic signatures, and enabled audit trail logging, it satisfies core 21 CFR Part 11 requirements for electronic records and signatures.
Can the ELSD be used without manual calibration?
Yes—the integrated ELSD features auto-optimization of nitrogen flow and drift tube temperature based on mobile phase composition and flow rate, eliminating routine manual tuning.
What safety certifications does the Pure C-835 hold?
CE-marked per Machinery Directive 2006/42/EC and Low Voltage Directive 2014/35/EU; compliant with EN 61010-1:2010 for laboratory equipment safety.
Is method transfer from analytical HPLC possible?
Yes—PureControl includes retention time scaling tools and gradient translation algorithms to facilitate linear velocity- and column volume-normalized method transfer from 4.6 mm ID analytical columns to preparative-scale systems.
