Agilent 1200 Nano HPLC System with G1315B Diode Array Detector

Overview

The Agilent 1200 Nano HPLC System is a high-precision, modular liquid chromatography platform engineered for low-flow, high-sensitivity applications—particularly in proteomics, metabolomics, and biomarker discovery workflows. This refurbished system operates on the principle of reversed-phase liquid chromatography (RPLC), where analytes are separated based on hydrophobic interactions with a stationary phase under precisely controlled gradient elution conditions. The integrated G1315B Diode Array Detector (DAD) enables simultaneous multi-wavelength UV-Vis absorbance detection across 190–800 nm, supporting spectral library matching, peak purity assessment, and quantitative analysis with high photometric accuracy. All modules—including the G2226A nano-pump (flow range: 10 nL/min to 100 µL/min), G1377A microplate autosampler (100-position capacity, 0.1–100 µL injection volume), G1316A column compartment (temperature control: 5–80 °C, ±0.15 °C stability), and G1379B vacuum degasser—are fully functional and calibrated to Agilent’s original factory specifications. The system was recently decommissioned from an active research laboratory and has undergone comprehensive refurbishment, including pump seal replacement, detector lamp validation, flow rate verification, and baseline noise characterization.

Key Features

• Modular architecture compliant with Agilent 1200 Series hardware standards, enabling seamless integration with existing lab infrastructure or future upgrades.
• G1315B DAD equipped with a 1024-element photodiode array and dual-beam optical design for enhanced signal-to-noise ratio and long-term photometric stability.
• G2226A nano-pump delivering ultra-low dispersion, pulse-free flow with <±0.1% RSD at 100 nL/min—critical for coupling with nano-electrospray ionization (nano-ESI) mass spectrometry.
• G1377A autosampler featuring needle-in-loop injection, washable needle seat, and programmable solvent rinse protocols to minimize carryover (<0.005%).
• Fully validated thermal management: G1316A column compartment maintains setpoint accuracy within ±0.15 °C over 24-hour operation, ensuring retention time reproducibility (RSD <0.2% over 10 injections).
• Refurbishment includes replacement of all wear-prone components (piston seals, check valves, lamp, flow cells), full leak testing, and electronic diagnostics per Agilent Service Manual Rev. D.

Sample Compatibility & Compliance

This system supports analytical-scale (1–4.6 mm ID) and nano-capillary (50–150 µm ID) columns, compatible with C18, C8, phenyl-hexyl, HILIC, and ion-exchange chemistries. It meets ASTM E2656-21 requirements for LC system suitability testing and supports USP chromatographic system suitability criteria (resolution, tailing factor, theoretical plates). Data acquisition and instrument control comply with FDA 21 CFR Part 11 when used with Agilent ChemStation Rev. B.04.03 or later (included), providing audit trail logging, electronic signatures, and user access controls. All refurbished units undergo GLP-aligned operational qualification (OQ) documentation, including pressure calibration, dwell volume measurement, and gradient delay verification.

Software & Data Management

The system ships with a dedicated Windows-based PC preloaded with Agilent ChemStation for LC 3D Systems (Rev. B.04.03 SP3), licensed for full data acquisition, processing, reporting, and method development. Raw data files (.D) are stored in vendor-native format and exportable to ASCII, CSV, or mzXML for third-party tools (e.g., MaxQuant, Skyline). Software configuration includes pre-installed instrument methods for common bioanalytical assays (e.g., peptide mapping, small-molecule quantitation), customizable report templates, and automated system suitability test (SST) execution. Audit trails record all method edits, sequence changes, and data reprocessing events with timestamp, user ID, and reason-for-change fields—fully traceable for regulatory submissions.

Applications

• Bottom-up proteomics: nano-LC separation of tryptic digests prior to LC-MS/MS analysis.
• Pharmaceutical impurity profiling: forced degradation studies and stability-indicating method development per ICH Q5C.
• Clinical biomarker validation: targeted quantitation of low-abundance proteins or metabolites in plasma/serum using isotope-labeled internal standards.
• Natural product isolation: fraction collection-guided purification with real-time DAD spectral feedback.
• Quality control of oligonucleotide therapeutics: separation of full-length vs. n−1/n−2 impurities using ion-pairing RPLC.

FAQ

Is this system compatible with mass spectrometry interfaces?
Yes—the G2226A nano-pump and low-dead-volume fluidic path support direct coupling to electrospray (ESI) and nanospray sources via standard nano-ESI emitters and zero-dead-volume fittings.
What documentation is provided with the refurbished unit?
Each system ships with a Certificate of Refurbishment, OQ report (including flow accuracy, pressure linearity, detector wavelength accuracy, and baseline noise), and a 6-month limited warranty covering parts and labor.
Can the software be upgraded to OpenLab CDS?
Yes—ChemStation data files are fully importable into Agilent OpenLab CDS v2.x or later; upgrade paths and licensing options are available upon request.
Are consumables and spare parts included?
No—columns, filters, frits, and solvents are not included, but we maintain inventory of genuine Agilent consumables (e.g., G1316-60005 column oven gaskets, G1315-60005 DAD flow cells) and provide bulk pricing for lab-wide procurement.
Do you offer installation and on-site training?
Yes—remote startup assistance is included; on-site commissioning and operator training (8 hours) are available as optional add-ons, delivered by Agilent-certified field application specialists.