Tailin Biotech 51-Well and 97-Well Most Probable Number (MPN) Quantitative Tray
| Brand | Tailin Biotech |
|---|---|
| Origin | Zhejiang, China |
| Manufacturer Type | OEM/ODM Manufacturer |
| Product Origin | Domestic (China) |
| Model | 51-Well / 97-Well MPN Quantitative Tray |
| Pricing | Upon Request |
Overview
The Tailin Biotech 51-Well and 97-Well Most Probable Number (MPN) Quantitative Tray is a standardized, single-use microbiological enumeration device engineered for rapid, culture-based detection and quantification of coliform bacteria in water samples. Designed to comply with the fundamental principles of the MPN method—statistical estimation based on presence/absence responses across serial dilutions—the tray integrates pre-aliquoted, dehydrated chromogenic or selective growth media into individual wells. When rehydrated with sample inoculum and sealed using a programmable quantitative sealing instrument, each well functions as an independent micro-culture chamber. Growth is indicated by color change (e.g., yellow for acid production, blue fluorescence under UV for β-glucuronidase activity), enabling visual or automated interpretation. This system eliminates manual tube preparation, reduces inter-operator variability, and delivers statistically robust enumeration results aligned with international standard methods including ISO 9308-1 (2014), EPA Method 1604, and Standard Methods for the Examination of Water and Wastewater (SM 9221).
Key Features
- Two standardized configurations: 51-well tray optimized for routine monitoring with high sensitivity in low-contamination scenarios; 97-well tray offering extended dynamic range for higher microbial loads and enhanced statistical confidence.
- Sterile, individually wrapped packaging ensures aseptic integrity prior to use—eliminating risk of cross-contamination during handling and storage.
- Robust polypropylene substrate with precision-molded wells designed for consistent capillary fill volume and uniform media rehydration kinetics.
- Validated structural integrity: Each sealed well withstands ≥8 kg of compressive force without leakage, rupture, or inter-well fluid migration—even under stacked incubation conditions.
- Optimized well geometry and seal interface ensure >99.9% sealing reliability when used with compatible programmable sealing instruments, minimizing false negatives due to evaporation or contamination.
Sample Compatibility & Compliance
The tray supports direct inoculation of untreated or membrane-filtered water samples—including drinking water, surface water, groundwater, recreational water, and wastewater effluents. Sample volumes are standardized per well (e.g., 10 mL, 1 mL, 0.1 mL) to maintain statistical validity of the MPN calculation. The system conforms to Good Laboratory Practice (GLP) requirements for traceability and documentation. While not inherently compliant with FDA 21 CFR Part 11, the tray is fully compatible with validated LIMS-integrated workflows that support electronic audit trails, user authentication, and data integrity controls required under ISO/IEC 17025 accreditation. Performance validation data aligns with ISO/IEC 17025:2017 clause 5.4.2 for method verification and demonstrates ≥95% confidence intervals across the specified detection range (as defined by the Thomas-Haldane MPN table).
Software & Data Management
The tray itself is hardware-agnostic but interoperates with proprietary image-analysis software from sealing instrument manufacturers for semi-automated colony counting and MPN table lookup. Raw well-readout data (positive/negative status per dilution series) can be exported in CSV or XML format for integration into validated laboratory information management systems (LIMS). Built-in batch tracking via alphanumeric lot codes enables full traceability from manufacturing to final result reporting. All performance qualification reports—including sterility testing (ISO 11737-1), seal integrity validation (ASTM F2096 bubble test equivalent), and shelf-life stability studies (24 months at 2–8°C)—are provided with each shipment.
Applications
- Routine compliance monitoring of potable water supplies per national regulatory frameworks (e.g., China’s GB 5749-2022, US EPA Safe Drinking Water Act).
- Environmental surveillance of source waters, bathing beaches, and aquaculture systems for Escherichia coli, thermotolerant coliforms, and total coliforms.
- Verification of disinfection efficacy in wastewater treatment plants and decentralized sanitation systems.
- Field-deployable water safety testing in resource-limited settings where centrifugation, filtration, or incubator access is constrained.
- Reference method validation and interlaboratory comparison studies requiring reproducible MPN endpoints.
FAQ
What is the shelf life of unopened trays, and how should they be stored?
Unopened trays retain full performance for 24 months when stored at 2–8°C in original packaging. Avoid freezing or exposure to ambient humidity above 60% RH.
Can the same tray be used for both total coliforms and E. coli detection?
Yes—when paired with appropriate dual-substrate media (e.g., modified mTEC or Colilert-18 formulations), the tray enables simultaneous differentiation and enumeration of both groups in a single assay.
Is calibration required before each use?
No calibration is needed; however, daily procedural controls (positive and negative reference strains) must be run alongside samples to verify media performance and operator technique.
Does the tray meet ISO 9308-1:2014 Annex A requirements for MPN quantification?
Yes—the 97-well configuration satisfies the minimum 100-well statistical requirement outlined in Annex A for high-confidence enumeration, while the 51-well version meets Annex B for routine monitoring applications.
Are custom well configurations or media formulations available?
Tailin Biotech offers OEM development services for application-specific media, well layouts, and packaging formats under NDA and ISO 13485-compliant manufacturing protocols.
