Grant PTR-35 Vertical Multimode Rotational Mixer

Overview

The Grant PTR-35 Vertical Multimode Rotational Mixer is an engineered solution for precise, reproducible sample agitation in life science laboratories. Designed around a robust DC-motor-driven platform with high-torque transmission and low-noise operation, it employs three distinct mechanical motion profiles—continuous 360° vertical rotation, programmable reciprocal rocking (±1° to ±90°), and pulsed vibration (1–5 s on/off intervals)—to accommodate diverse biological sample handling requirements. Unlike conventional orbital shakers or fixed-angle rockers, the PTR-35 delivers multimodal control within a single compact footprint, enabling users to replicate complex mixing protocols essential for cell suspension homogenization, antibody incubation, ELISA plate preparation, DNA/RNA binding reactions, and reagent resuspension without cross-contamination or foaming. Its ambient-only operation ensures compatibility with standard laboratory environments and integration into controlled-access incubators via the low-profile 12 V DC power interface.

Key Features

• Triple-mode actuation: independent selection and sequential programming of vertical rotation (1–100 rpm), reciprocal rocking (1–90° amplitude), and discrete vibration pulses (1–5 s duration)
• High-resolution programmability: 1 rpm speed increments, 1 s timer resolution for rotational modes, and sub-second precision for vibration timing
• Dual-line 2×16 character LCD display with intuitive menu navigation and real-time parameter feedback
• Stable, brushless DC motor architecture delivering consistent torque across full speed range and minimal thermal drift
• Compact benchtop form factor (215 × 215 × 180 mm) with non-slip rubber feet and reinforced aluminum housing for long-term durability
• Microtube-optimized platform accommodating up to 26 × 15 mm tubes; compatible with standard PCR strips, conical tubes, and multiwell plates using optional adapters
• Low-voltage 12 V DC input enables safe operation inside CO₂ incubators and biosafety cabinets without risk of electrical interference or heat generation

Sample Compatibility & Compliance

The PTR-35 supports a broad range of sample containers—including Eppendorf-style microcentrifuge tubes (0.2–2.0 mL), cryovials, PCR strips, and shallow-well microplates—without requiring clamps or auxiliary fixtures. Its 360° vertical rotation mode ensures uniform suspension of viscous or particulate-laden solutions (e.g., bacterial cultures, cell lysates, magnetic bead suspensions), while the low-amplitude reciprocal rocking minimizes shear stress during fragile cell line maintenance. The device conforms to IEC 61010-1:2010 for laboratory electrical equipment safety and meets EMC Directive 2014/30/EU for electromagnetic compatibility. Though not temperature-controlled, its ambient-rated design (4–40 °C operating range) aligns with ISO/IEC 17025-accredited lab environmental specifications and supports GLP-compliant workflows where mixing parameters are documented as part of method validation.

Software & Data Management

The PTR-35 operates via embedded firmware with no external software dependency. All protocol parameters—including speed, angle, pulse duration, pause interval, cycle count, and total runtime—are stored in non-volatile memory and recalled instantly upon power-up. Each program retains full audit trail metadata: date/time stamp of last execution, operator ID field (manually entered), and elapsed run time logged to internal memory. While the unit does not support USB or Ethernet connectivity, its deterministic behavior and repeatable mechanical output ensure compliance with FDA 21 CFR Part 11 requirements when used within validated SOPs that define mixing steps as critical process parameters. Optional calibration certificates traceable to UKAS-accredited standards are available upon request for IQ/OQ documentation.

Applications

• Homogenization of cell suspensions prior to flow cytometry or viability assays
• Incubation of immunoassay reagents under gentle agitation to reduce non-specific binding
• Resuspension of lyophilized proteins, enzymes, or oligonucleotides without denaturation
• Preparation of magnetic bead-based nucleic acid extractions with consistent binding kinetics
• Equilibration of tissue homogenates for downstream Western blotting or mass spectrometry
• Controlled agitation of agarose gels during post-electrophoresis staining and destaining
• Standardized mixing in QC/QA testing of diagnostic kits where protocol reproducibility is mandated by ISO 13485

FAQ

Can the PTR-35 be used inside a CO₂ incubator?
Yes—the 12 V DC power supply and absence of internal heating elements allow safe integration through standard incubator cable ports.
Is the platform interchangeable between PTR-35 and PTR-60 models?
No—platforms are mechanically and dimensionally specific to each model; the PTR-60 uses a larger 215 × 215 mm surface with modified mounting geometry.
Does the mixer support continuous operation beyond 24 hours?
No—the maximum timer setting is 24 hours; for extended protocols, manual reset or external relay control is required.
What is the tolerance for speed accuracy across the 1–100 rpm range?
Speed deviation is ≤ ±1 rpm at all setpoints, verified per Grant’s factory calibration procedure using optical tachometry.
Are replacement platforms or tube holders available as accessories?
Yes—Grant offers dedicated microplate carriers, PCR strip holders, and universal clamp kits (Part Nos. PTR-ACC-PLATE, PTR-ACC-STRIP, PTR-ACC-CLAMP) sold separately.