Shimadzu LC-2010CHT Used High-Performance Liquid Chromatograph
| Brand | Shimadzu |
|---|---|
| Origin | Japan |
| Model | LC-2010CHT |
| Instrument Category | HPLC System |
| Solvent Delivery | Dual-plunger parallel pump (main piston 10 µL, auxiliary piston 5 µL) |
| Flow Rate Range | 0.001–5.0 mL/min (at 1–35 MPa) |
| Flow Accuracy | ±1% (at 1 mL/min, 3–10 MPa, water, 20 °C) |
| Flow Precision | ≤0.075% RSD or ≤0.02 min SD (whichever is greater |
| Pressure Accuracy | ±2% or ±0.5 MPa (whichever is greater) |
| Gradient Capability | 4-solvent low-pressure mixing |
| Gradient Composition Range | 0–100% in 0.1% increments |
| Gradient Accuracy | ±0.5% (acetone/water, binary, 0.1–2 mL/min, 1–20 MPa) |
| Gradient Precision | ±0.1% (same conditions) |
| Degasser | Membrane-based online degassing, 5 channels (4 for mobile phase, 1 for autosampler wash solvent) |
| Autosampler | Injection volume 0.1–100 µL (standard), up to 2000 µL (optional) |
| sample capacity | 350 × 1 mL vials, 140 × 1.5 mL vials, or 100 × 4.0 mL vials |
| injection repeatability | <0.3% RSD (10 µL) |
| carryover | <0.01% (naphthalene test) |
| injection accuracy | ±1% (50 µL, n=6) |
| max. injections per sample | 99 |
| run time range | 0.01–9999.9 min (0.01-min increment) |
| programmable steps | up to 202 |
| Column Oven | Peltier-heated/cooled |
| temp. control range | 15 °C below ambient to 60 °C |
| stability | ±0.1 °C |
| UV-Vis Detector | Deuterium lamp source |
| wavelength range | 190–600 nm |
| bandwidth | 8 nm |
| wavelength accuracy | ±1 nm |
| wavelength precision | ±0.1 nm |
| drift | ≤1 × 10⁻⁴ AU/h (250 nm, empty cell) |
| noise | ≤±0.25 × 10⁻⁵ AU (250 nm, empty cell, 1 s time constant) |
| flow cell path length | 10 mm (8 µL standard), 5 mm (2.4 µL semi-prep) |
| dual-wavelength detection | any two wavelengths from 190–370 nm and 371–600 nm |
| Sample Cooler | Direct-cooling type |
| temperature setpoint | 4–40 °C |
| System Controller | Integrated system controller with embedded software interface |
| Warranty | 6 months |
| Operational Age | ≥7 years |
| Compliance | Designed to support GLP/GMP workflows |
Overview
The Shimadzu LC-2010CHT is a fully integrated, high-performance liquid chromatography (HPLC) system originally engineered for robust routine analysis and method validation in regulated environments. Based on Shimadzu’s proven LC-2010 platform, this used instrument implements low-pressure gradient mixing, dual-plunger parallel pumping, and real-time pressure monitoring to deliver precise solvent delivery across wide flow and pressure ranges (0.001–5.0 mL/min at up to 35 MPa). Its modular architecture integrates a vacuum membrane degasser with five independent channels—four dedicated to mobile phase lines and one reserved for autosampler wash solvent—ensuring consistent baseline stability and minimizing bubble-related retention time shifts. The system employs a Peltier-based column oven with active heating and cooling, enabling precise thermal control (±0.1 °C) from 15 °C below ambient to 60 °C—critical for method transfer and retention time reproducibility. Coupled with a high-stability UV-Vis detector featuring dual-wavelength acquisition and sub-nanometer wavelength precision, the LC-2010CHT supports quantitative and qualitative analysis of pharmaceuticals, environmental analytes, food contaminants, and natural product extracts.
Key Features
- Dual-plunger parallel pump design with active (10 µL) and auxiliary (5 µL) pistons ensures low-pulsation flow and enhanced durability over extended operation.
- Low-pressure quaternary gradient unit enables precise, programmable mixing of up to four solvents with 0.1% resolution and ±0.1% composition precision under defined operating conditions.
- Integrated sample cooler (direct-cooling type) maintains vial temperatures between 4–40 °C—essential for thermally labile compounds and long unattended sequences.
- Autosampler with <0.3% RSD injection repeatability (10 µL) and <0.01% carryover (naphthalene test) meets stringent requirements for trace-level quantitation and high-throughput QC labs.
- UV-Vis detector equipped with deuterium lamp, 190–600 nm spectral coverage, and dual-channel synchronous acquisition supports peak purity assessment and multi-analyte detection without hardware switching.
- Embedded system controller provides native method storage, sequence management, and real-time diagnostics—reducing dependency on external PCs during routine operation.
Sample Compatibility & Compliance
The LC-2010CHT accommodates standard 1 mL, 1.5 mL, and 4 mL autosampler vials (up to 350 positions), supporting diverse sample matrices including aqueous extracts, organic solvent suspensions, plasma supernatants, and purified fractions. Its column oven accepts up to two 30 cm columns (ID 2.1–4.6 mm), facilitating method development with multiple stationary phases. From a regulatory perspective, the system was originally designed to align with ISO/IEC 17025 and ASTM E2655 guidance for chromatographic performance verification. When operated with validated firmware (e.g., LabSolutions LC-MS v5.9 or later) and paired with audit-trail-enabled acquisition software, it supports compliance with FDA 21 CFR Part 11, EU Annex 11, and ICH Q2(R2) analytical procedure validation requirements. All mechanical and electronic components—including pressure transducers, temperature sensors, and lamp intensity monitors—are factory-calibrated and traceable to JIS standards.
Software & Data Management
While the LC-2010CHT ships with its original embedded controller, full data acquisition, processing, and reporting require connection to Shimadzu’s LabSolutions LC/GC software suite. LabSolutions v5.9+ provides comprehensive tools for method development, system suitability testing (SST), peak integration, calibration curve generation, and electronic signature support. Raw data files (.lcd) are stored in a structured, non-proprietary format compliant with long-term archival standards. Audit trail functionality records all user actions—including parameter changes, sequence edits, and report exports—with timestamps and operator IDs. Data integrity is further reinforced through role-based access control, password-protected method locking, and automated backup scheduling. For laboratories implementing paperless workflows, the system integrates seamlessly with LIMS platforms via ASTM E1384 or HL7-compliant interfaces.
Applications
This refurbished LC-2010CHT system is routinely deployed in quality control laboratories for assay validation of active pharmaceutical ingredients (APIs), dissolution testing per USP , and residual solvent analysis by headspace-GC/HPLC correlation. In academic and contract research settings, it supports natural product isolation monitoring, metabolite profiling in plant extracts, and stability-indicating method development for biologics. Environmental labs use its low-drift UV detection and gradient flexibility for EPA Method 8330B (phthalates) and ASTM D7087 (petroleum hydrocarbons). Its sample cooler and precise temperature control also make it suitable for analyzing heat-sensitive compounds such as peptides, oligonucleotides, and certain vitamins (e.g., B12, C) where column and sample thermal degradation must be minimized.
FAQ
Is this LC-2010CHT unit supplied with validated software and electronic records capability?
Yes—when configured with LabSolutions LC-MS v5.9 or later and operated under documented procedures, the system supports 21 CFR Part 11 compliance, including electronic signatures, audit trails, and data archiving.
What maintenance history is available for this used instrument?
Each unit undergoes full functional verification prior to shipment, including pump linearity, detector linearity and noise testing, autosampler precision evaluation, and column oven thermal mapping. Full service logs are provided upon request.
Can the system be upgraded to support modern networked operation?
Yes—the controller supports Ethernet connectivity and can be integrated into local area networks for remote monitoring and centralized data collection using Shimadzu’s Network Manager utility.
Does the 6-month warranty cover consumables and lamps?
The warranty covers all core hardware components and electronics. UV lamps, fuses, seals, and other wear items are excluded but available at cost-effective pricing through authorized distributors.
Is method transfer from newer Shimadzu systems feasible?
Yes—method parameters (flow rates, gradients, detection wavelengths, oven temperatures) are directly transferable. Minor retention time adjustments may be required due to column aging or minor pump seal wear, both assessable during initial qualification.
