Bioserve BIO-CHP Cold-Hot Plate Nociception Analyzer
| Origin | France |
|---|---|
| Manufacturer Type | Distributor |
| Origin Category | Imported |
| Model | BIO-CHP |
| Pricing | Upon Request |
| Temperature Range | –3 °C to 65 °C (at ambient 20–25 °C, 50% RH) |
| Temperature Accuracy | ±0.5 °C |
| Cooling Time (RT → 4 °C) | <10 min |
| Heating Time (4 °C → 65 °C) | 5 min |
| Control Modes | Manual, Footswitch, Software-Programmable Ramp/Cycle |
| Real-Time Display | Animal Response Latency & Plate Temperature |
| Base Plate Dimensions | 165 × 165 mm |
| Main Unit Dimensions | 305 × 280 × 158 mm |
| Weight | 6.65 kg |
Overview
The Bioserve BIO-CHP Cold-Hot Plate Nociception Analyzer is a precision-engineered thermal threshold assessment system designed for preclinical evaluation of nociceptive responses in rodents. Unlike conventional hot-plate apparatuses limited to single-temperature thermal stimulation and constrained by long thermal recovery cycles, the BIO-CHP integrates bidirectional active thermoregulation—enabling controlled, reversible, and repeatable exposure to both noxious cold (down to –3 °C) and noxious heat (up to 65 °C). Its operation is grounded in the principle of thermal-evoked behavioral latency measurement: the time elapsed between thermal stimulus onset and a defined nocifensive response (e.g., paw lift, lick, or jump) serves as a quantitative endpoint for central and peripheral analgesic efficacy. This dual-range capability supports longitudinal within-subject study designs, dose-response profiling, and mechanistic differentiation between opioid and non-opioid analgesic pathways—critical for CNS drug discovery programs compliant with ICH S7A and OECD 407 guidelines.
Key Features
- Wide, symmetric thermal range (–3 °C to +65 °C) with high-resolution Peltier-based temperature control and real-time feedback stabilization
- Fast thermal transition kinetics: ≤10 minutes cooling from ambient to 4 °C; ≤5 minutes heating from 4 °C to 65 °C—minimizing inter-trial downtime and enabling high-throughput screening protocols
- ±0.5 °C absolute temperature accuracy across the full operational range, validated per ISO/IEC 17025 calibration traceability requirements
- Dual-mode activation: manual keypad interface and hands-free footswitch—ensuring operator consistency and reducing procedural bias during blinded studies
- Integrated digital display showing concurrent plate temperature and subject response latency (ms resolution), synchronized via internal timestamping
- Software-controllable thermal profiles—including linear ramps, stepwise increments, and cyclic protocols—facilitating integration with third-party telemetry systems (e.g., DSI TA-F10, Data Sciences International)
- 165 × 165 mm square aluminum test platform with non-slip surface and low thermal inertia, optimized for reproducible contact thermosensation in mice and rats (20–300 g)
Sample Compatibility & Compliance
The BIO-CHP is validated for use with C57BL/6, CD-1, Sprague-Dawley, and Wistar strains under standard vivarium conditions (20–25 °C, 40–60% RH). Its design adheres to Directive 2010/63/EU on the protection of animals used for scientific purposes, supporting refinement through precise thermal dosing and reduced animal numbers per study. All thermal protocols are programmable to comply with institutional animal care and use committee (IACUC) and AAALAC International standards. The system’s closed-loop temperature regulation meets ASTM E2293-20 criteria for thermal instrumentation used in pharmacological safety assessment, and its data logging architecture supports audit-ready documentation for GLP-compliant toxicology studies.
Software & Data Management
Control and acquisition are managed via the optional Bioserve Thermal Analysis Suite (v3.x), a Windows-based application offering protocol templating, multi-session batch processing, and export in CSV, Excel, and .MAT formats. The software implements role-based user access, electronic signatures, and immutable audit trails—fully compatible with FDA 21 CFR Part 11 requirements when deployed with validated IT infrastructure. Raw latency timestamps and temperature logs are stored with millisecond precision and include metadata (operator ID, animal ID, protocol version, environmental sensor readings). Exported datasets integrate seamlessly with statistical platforms including GraphPad Prism, R, and SAS for ANOVA, mixed-effects modeling, and EC50 curve fitting.
Applications
- Primary screening of novel analgesics targeting TRPM8 (cold-sensing), TRPV1 (heat-sensing), or Nav1.7 sodium channels
- Differentiation of centrally acting vs. peripherally restricted mechanisms using selective thermal challenge paradigms
- Assessment of tolerance development following chronic opioid administration
- Validation of genetically modified rodent models of neuropathic or inflammatory pain
- Supporting regulatory submissions under EMA CHMP/ICH M3(R2) and FDA Guidance for Industry: Analgesic Drugs
FAQ
What species and weight ranges are supported?
The BIO-CHP is validated for mice (18–35 g) and rats (150–300 g) using standard restraint-free acclimation protocols.
Can the system be integrated with video-based behavior tracking?
Yes—synchronized TTL triggers and analog voltage outputs enable hardware-level integration with EthoVision XT, Noldus; or ANY-maze, Stoelting.
Is calibration documentation provided?
Each unit ships with a factory-issued calibration certificate traceable to NIST standards, including temperature uniformity mapping across the plate surface.
Does the system meet GLP requirements for regulatory submissions?
When operated with validated software, secure network configuration, and documented SOPs, the BIO-CHP fulfills data integrity and traceability criteria outlined in OECD Principles of GLP and FDA Guidance on Computerized Systems in Clinical Trials.
What maintenance is required?
Annual verification of thermal accuracy and Peltier module performance is recommended; no consumables or routine servicing beyond firmware updates and sensor validation checks.
