Zuofei Chenghuang 96-Channel Fully Automated Liquid Handling Workstation
| Brand | Zuofei |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Original Equipment Manufacturer (OEM) |
| Country of Origin | China |
| Model | Chenghuang |
| Plate Positions | 4 (customizable to 5 or 6) |
| Pipetting Accuracy | ±0.3–3.8% CV |
| Pipetting Range | 2–1000 µL |
| Throughput | 96 samples per cycle |
| Dimensions | 580 × 325 × 548 mm |
Overview
The Zuofei Chenghuang 96-Channel Fully Automated Liquid Handling Workstation is an integrated benchtop platform engineered for high-precision, high-throughput liquid transfer in life science laboratories. Based on positive-displacement and air-displacement pipetting principles—optimized for reproducibility across viscous, volatile, and low-volume aqueous and biological solutions—the system delivers consistent performance in standardized SBS-format microplates (96-well, 384-well, and custom configurations). Designed for walk-away automation, the Chenghuang workstation executes complex multi-step protocols—including serial dilution, plate replication, sample normalization, reagent dispensing, and assay setup—without manual intervention. Its modular architecture supports seamless integration into existing laboratory workflows, particularly where GLP-compliant documentation, audit-ready operation logs, and traceable liquid handling are required.
Key Features
- 96-channel parallel pipetting head enabling full 96-well plate transfers in a single aspiration/dispense cycle; 384-well plates completed in four sequential steps with positional accuracy ≤±0.1 mm
- Automated tip loading and ejection via motorized tip rack handling—compatible with universal SBS-compliant low-retention tips (10–1000 µL)
- Adjustable pipetting range from 2 µL to 1000 µL with volumetric accuracy maintained within ±0.3–3.8% CV (dependent on volume and tip type), validated per ISO 8655-6 guidelines
- Four standard SBS plate positions (expandable to five or six upon configuration); all positions support independent Z-height calibration and collision avoidance logic
- Programmable deck layout: users define source/destination plate types, reagent locations, and workflow sequence via intuitive drag-and-drop interface
- Robust mechanical design with linear encoders and closed-loop stepper motor control ensures long-term repeatability and minimal maintenance intervals
Sample Compatibility & Compliance
The Chenghuang system accommodates a broad spectrum of sample matrices—including genomic DNA, cDNA, PCR master mixes, cell lysates, serum, and antibody solutions—across standard ANSI/SBS microplate formats (e.g., PCR plates, deep-well blocks, assay plates). It complies with key international standards for laboratory automation: ISO/IEC 17025:2017 (for testing laboratories), ISO 8655-6 (volumetric accuracy verification), and ASTM E2876-13 (guidance for automated liquid handler qualification). For regulated environments, optional software modules support 21 CFR Part 11 compliance—including electronic signatures, user role-based access control, and immutable audit trails of all pipetting events and parameter changes.
Software & Data Management
The proprietary Zuofei Control Suite provides a Windows-based graphical interface built on a deterministic real-time execution engine. Preconfigured protocol templates cover common applications such as qPCR setup, NGS library normalization, ELISA reagent dispensing, and HTS compound dilution series. All protocols are exportable as XML-based method files for version control and cross-platform sharing. Raw log data—including timestamped volume records, tip usage history, error codes, and motion trajectories—is stored in SQLite databases with optional export to CSV or LIMS-compatible XML. The software supports direct integration with third-party LIMS and ELN systems via RESTful API endpoints and OPC UA connectivity.
Applications
- Genomics & Molecular Biology: High-fidelity PCR/qPCR master mix preparation; automated nucleic acid extraction from 96-well magnetic bead plates; dual-indexed NGS library pooling and normalization
- Drug Discovery & Screening: Compound plate replication (96→384, 384→1536), dose-response matrix generation, and high-content assay plate formatting
- Diagnostics & Clinical Labs: IVD kit assembly (e.g., ELISA component dispensing), clinical sample aliquoting under ISO 15189 workflows, and pathogen detection panel setup
- Biotherapeutics Development: Monoclonal antibody titer screening, CHO cell line clone selection plates, and bioprocess intermediate sampling
- Forensics & Public Health: DNA extraction plate staging for STR analysis, RNA isolation from clinical swabs, and multiplexed pathogen RT-qPCR plate setup
FAQ
What plate formats does the Chenghuang workstation support?
Standard SBS-compliant formats including 96-well, 384-well, and deep-well (V- or U-bottom) plates; custom plate carriers available upon request.
Can the system perform gradient dilutions or serial transfers?
Yes—via programmable multi-step dispense routines with dynamic volume adjustment and cross-plate carryover compensation.
Is validation documentation provided for GxP environments?
Yes—IQ/OQ documentation packages, including test scripts, acceptance criteria, and raw data worksheets, are supplied with each unit.
Does the workstation support barcode scanning for sample tracking?
Optional barcode reader module (USB-HID compatible) enables plate-level identification and LIMS synchronization.
What maintenance is required for long-term reliability?
Quarterly tip ejector calibration and annual pipetting accuracy verification using gravimetric methods per ISO 8655-6.
