Analytik Jena InnuPure C16 Automated Magnetic Bead-Based Nucleic Acid Purification System
| Brand | Analytik Jena |
|---|---|
| Origin | Germany |
| Model | InnuPure C16 |
| Purification Principle | Magnetic Bead-Based (Surface-Modified Nanoscale Beads) |
| Throughput | 1–16 Samples per Run |
| Maximum Sample Volume | 2 mL Whole Blood |
| Elution Volume Range | 50–500 µL (10 µL Increments) |
| Purification Time | As Fast as 40 Minutes |
| Heating Capability | Up to 70 °C |
| Pipetting Volume | 1000 µL Filtered Tips |
| Control Interface | 5.7-inch Color Touchscreen (HID-Pro 320) |
| Dimensions (W × H × D) | 380 × 435 × 530 mm |
| Weight | 28 kg |
| Connectivity | RS-232, USB |
| Compliance | Designed for GLP/GMP-aligned workflows |
Overview
The Analytik Jena InnuPure C16 is a compact, fully automated nucleic acid purification system engineered for precision, reproducibility, and operational simplicity in molecular biology laboratories. It employs magnetic bead-based separation technology—leveraging surface-modified nanoscale paramagnetic particles that selectively bind nucleic acids under controlled chaotropic and pH conditions. Unlike centrifugation- or column-based methods, the InnuPure C16 uses a rotating magnetic disk beneath the sample tubes to immobilize beads firmly at the tube bottom, enabling clean aspiration of supernatant without carryover. This physical separation mechanism eliminates residual magnetic particles in eluates—a critical requirement for downstream applications including qPCR, NGS library preparation, and digital PCR. The system operates as a standalone instrument with no PC dependency, integrating thermal lysis (up to 70 °C), precise liquid handling, and on-deck reagent activation—all within a single, self-contained workflow.
Key Features
- Magnetic Bead Integrity Assurance: Proprietary magnetic drive system positions beads uniformly at the tube base prior to aspiration, ensuring zero bead carryover into eluate—validated by spectrophotometric and fluorometric assessment across >100 clinical and environmental sample types.
- Cross-Contamination Prevention: Integrated 1000 µL filtered tips with aerosol-resistant design; sealed reagent strips pierced automatically only during aspiration—preventing premature reagent exposure and ambient contamination.
- Modular Thermal Control: Programmable heating module maintains consistent temperature profiles (room temperature to 70 °C) across all positions, enhancing cell lysis efficiency for Gram-positive bacteria, FFPE tissue, and fibrous plant material.
- Touchscreen-Driven Simplicity: 5.7-inch high-resolution HID-Pro 320 interface enables one-touch program launch; preloaded protocols are organized by sample type and kit—no scripting or software installation required.
- Service-Free Liquid Handling: Five maintenance-free servo-driven stainless-steel pistons deliver long-term volumetric accuracy (CV < 3% at 200 µL) without calibration drift or seal replacement over 10,000 cycles.
- Full Accessibility Design: Front-hinged access door opens 180°; removable stainless-steel sample deck supports autoclaving or chemical decontamination; optional UV-C lamp (254 nm) provides internal chamber sterilization between runs.
Sample Compatibility & Compliance
The InnuPure C16 supports diverse input matrices—including whole blood (up to 2 mL), buccal swabs, stool, soil, bacterial pellets, viral lysates, FFPE sections, and forensic trace evidence—without hardware modification. Each application is matched to a CE-IVD or RUO-grade innuPREP kit (e.g., innuPREP Virus DNA/RNA Kit, innuPREP Forensic DNA Kit), all manufactured under ISO 13485-certified processes. The system architecture aligns with key regulatory expectations: electronic logs capture timestamped run parameters, user IDs, and error events; optional integration with LIMS via RS-232 or USB enables 21 CFR Part 11-compliant audit trails when deployed in FDA-regulated environments. All protocols adhere to ISO/IEC 17025 principles for method validation, and performance data meet minimum recovery thresholds defined in ISO 20387 for biobanking.
Software & Data Management
No external computer is required—the InnuPure C16 runs embedded firmware with built-in protocol library management. Users select from >12 validated kits directly on the touchscreen; each program defines tip sequence, heating ramp, mixing cycles, wash stringency, and elution volume (adjustable in 10 µL increments from 50–500 µL). Firmware updates are delivered free-of-charge via USB and include new kit support, enhanced error diagnostics, and improved tip wear compensation algorithms. Raw log files (CSV format) record every actuator movement, temperature reading, and magnetic field activation event—enabling full retrospective analysis. For centralized labs, optional HID-Pro Connect software allows remote monitoring of instrument status and queue management across multiple C16 units.
Applications
The system serves core needs in clinical diagnostics, translational research, food safety testing, and forensic genomics. It delivers consistent yields from low-input samples (e.g., 10⁴ CFU/mL bacterial cultures) and inhibitors-rich matrices (e.g., humic acid–contaminated soil or heme-rich blood). Validated use cases include: extraction of cfDNA from plasma prior to liquid biopsy sequencing; isolation of intact viral RNA from nasopharyngeal swabs under BSL-2 conditions; recovery of degraded DNA from formalin-fixed tissues for STR profiling; and parallel purification of plasmid DNA and genomic DNA from E. coli cultures for CRISPR construct validation. Its 40-minute runtime for 16 samples supports high-turnaround pathology labs, while single-sample mode accommodates urgent STAT requests without batch delay.
FAQ
Does the InnuPure C16 require connection to a computer to operate?
No—operation is fully autonomous via the integrated 5.7-inch color touchscreen. No driver installation, network configuration, or third-party software is needed.
Can I use third-party magnetic bead kits with this system?
The InnuPure C16 is optimized for innuPREP kits. While mechanical compatibility exists, performance validation (recovery, purity, reproducibility) applies exclusively to Analytik Jena–qualified reagents.
How is carryover contamination prevented between runs?
Through a three-tier strategy: filtered tips with aerosol barriers, automatic tip ejection into waste, and UV-C irradiation (optional) combined with removable, autoclavable stainless-steel deck.
What is the maximum eluate volume, and can it be customized per sample?
Elution volume is programmable from 50 µL to 500 µL in 10 µL steps—and is uniform across all processed samples within a single run.
Is service support available outside Germany?
Yes—Analytik Jena maintains certified service partners across North America, EMEA, and APAC regions, with spare parts logistics supporting <48-hour dispatch for critical components.
