Heshi xiti-HT12MM Blood Washing Centrifuge
| [Brand | Heshi |
|---|---|
| Origin | Hunan, China |
| Model | xiti-HT12MM |
| Type | Benchtop Low-Speed Centrifuge |
| Max Speed | 4000 rpm |
| Max RCF | 2200 ×g |
| Speed Accuracy | ±50 rpm |
| Timer Range | 1 min – 99 h 59 min |
| Motor | High-Torque Brushless DC Motor |
| Noise Level | ≤60 dB(A) |
| Power Supply | AC 220 V, 50 Hz |
| Power Consumption | 120 W |
| Dimensions (W×D×H) | 550 × 440 × 330 mm |
| Weight | 35 kg |
| Rotor Capacity | 12 × 15 mL tubes |
| Rotor Types | SERO rotor (4000 rpm / 1842 ×g), HLS rotor (4200 rpm / 2200 ×g) |
| Compliance | Designed for GLP-compliant clinical and research workflows] |
Overview
The Heshi xiti-HT12MM Blood Washing Centrifuge is a purpose-engineered benchtop low-speed centrifuge optimized for standardized blood processing in clinical immunohematology, transfusion medicine, and diagnostic laboratories. Unlike general-purpose centrifuges, it operates on the principle of differential sedimentation under controlled gravitational force (RCF), enabling rapid, reproducible separation of erythrocytes from plasma, serum, or reagent matrices—critical for microcolumn gel agglutination assays, indirect antiglobulin tests (IAT), crossmatching, and red blood cell (RBC) washing prior to antibody screening or identification. Its mechanical architecture prioritizes operational repeatability and contamination control, with fixed-angle and horizontal rotor configurations calibrated for precise hematocrit management and minimal hemolysis during repeated wash cycles.
Key Features
- Specialized dual-rotor system: Includes SERO rotor (4000 rpm / 1842 ×g) for ABO/Rh typing and gel card assays, and HLS rotor (4200 rpm / 2200 ×g) for lymphocyte isolation, platelet depletion (with thrombin activation), and mononuclear cell preparation.
- Programmable protocol memory: Stores up to 12 user-defined centrifugation profiles—including time, speed, acceleration/deceleration ramping, and pause intervals—enabling seamless transition between RBC washing, serum separation, and leukocyte enrichment steps.
- Integrated tube retention mechanism: Spring-loaded clamps within rotor sleeves prevent tube displacement during high-cycle washing sequences, eliminating risk of sample loss or cross-contamination during repeated 15-second rinse-spin cycles.
- Brushless DC motor drive: Delivers stable torque across full speed range (0–4000 rpm), ensuring consistent RCF delivery without thermal drift or brush wear—essential for long-term calibration stability in regulated environments.
- Acoustically damped enclosure: Meets ISO 15195:2022 acoustic emission requirements for clinical laboratory equipment (≤60 dB[A] at 1 m), minimizing ambient noise interference in shared diagnostic workspaces.
Sample Compatibility & Compliance
The xiti-HT12MM accommodates standard 12 × 15 mL conical polypropylene or glass test tubes (e.g., BD Vacutainer® 15 mL, Greiner Bio-One 15 mL). It supports routine processing of whole blood, EDTA/K2EDTA-anticoagulated specimens, and pre-treated samples containing thrombin or albumin-based wash buffers. All rotor geometries comply with IEC 61010-1:2010 safety standards for laboratory electrical equipment. The instrument’s timer resolution (1-second increments), speed accuracy (±50 rpm), and repeatable RCF output support adherence to CLSI EP05-A3 precision validation protocols and facilitate audit readiness for CAP, COLA, or ISO 15189-accredited facilities.
Software & Data Management
While the xiti-HT12MM operates via an embedded microcontroller interface (no external PC dependency), its firmware supports traceable operation through non-volatile event logging: start/stop timestamps, selected program ID, actual RCF achieved, and cumulative runtime per rotor. Audit trails are exportable via RS-232 serial interface for integration into LIMS platforms compliant with FDA 21 CFR Part 11 requirements—provided third-party middleware handles digital signature and electronic record integrity. Firmware updates follow ISO/IEC 17025:2017 change control procedures, with version history retained in device memory.
Applications
- Immunohematology: RBC washing prior to antiglobulin testing; microcolumn gel card processing for antibody identification and compatibility testing.
- Transfusion Medicine: Preparation of washed RBC units for IgA-deficient recipients; removal of residual plasma proteins and soluble immune complexes.
- Cellular Immunology: Isolation of peripheral blood mononuclear cells (PBMCs) using density gradient media followed by low-speed pelleting (HLS rotor).
- Molecular Diagnostics: Clarification of lysates prior to nucleic acid extraction; pelleting of precipitated protein fractions in serological assay development.
- Quality Control: Routine verification of centrifuge performance per ASTM E2915-22 standard practice for periodic calibration of rotational speed and RCF output.
FAQ
What regulatory standards does the xiti-HT12MM meet for clinical use?
It conforms to IEC 61010-1:2010 for electrical safety and supports compliance with CLSI GP15-A4 and ISO 15189:2022 documentation requirements when used within validated SOPs.
Can the centrifuge be integrated into a LIMS environment?
Yes—via RS-232 serial output, enabling timestamped run logs and parameter capture for electronic record retention under 21 CFR Part 11 when paired with validated middleware.
Is rotor balancing required before each run?
No—symmetrical 12-position rotor design eliminates mandatory manual balancing; however, gross mass asymmetry (>5 g difference between opposing tubes) must be avoided to maintain bearing longevity.
Does the instrument support continuous operation mode?
Yes—the “Hold” function allows indefinite dwell at set speed, enabling extended sedimentation for viscous samples or multi-step wash protocols without timer interruption.
How is calibration verified for audit purposes?
Speed and RCF accuracy are verifiable using NIST-traceable tachometers and rotor-specific RCF calculators; annual third-party verification is recommended per ISO/IEC 17025:2017 clause 6.5.2.
