Taicang Jiangsu HZQ-F100 Dual-Layer Full-Temperature Orbital Shaker Incubator

Overview

The Taicang Jiangsu HZQ-F100 Dual-Layer Full-Temperature Orbital Shaker Incubator is an engineered platform for controlled microbial growth, cell culture expansion, and biochemical reaction kinetics under precisely regulated thermal and mechanical conditions. Designed around a robust air-bath heating architecture with PID-controlled forced-air circulation, the unit maintains stable temperature setpoints from 5°C above ambient to 60°C—enabling applications ranging from psychrophilic bacterial cultivation to mesophilic yeast fermentation. Its orbital shaking mechanism operates on the Couette flow principle, delivering uniform, low-shear agitation ideal for suspension cultures, enzyme assays, and solubility studies. Unlike water-jacketed systems, the air-bath design eliminates condensation risks, simplifies maintenance, and ensures rapid thermal equilibration across both shelves—critical for reproducible batch-to-batch performance in GLP-compliant laboratories.

Key Features

• Dual independent shelves (420 × 300 mm each) support parallel experiments with identical or divergent protocols—reducing throughput bottlenecks in high-volume screening workflows.
• Adjustable orbital frequency (40–280 rpm) with digital feedback control ensures consistent motion profiles across load variations; no manual recalibration required after flask placement.
• Precision temperature regulation (±0.1°C accuracy, ±0.5°C uniformity) validated per ISO 17025 traceable calibration procedures, supporting compliance with USP <1058> analytical instrument qualification guidelines.
• LED-display interface with intuitive keypad navigation provides real-time monitoring of setpoint, actual temperature, speed, and remaining timer duration—no software dependency for basic operation.
• Integrated 8 W LED interior lighting enables visual inspection without opening the chamber, minimizing thermal disturbance and contamination risk during routine monitoring.
• Structural chassis fabricated from cold-rolled steel with epoxy-coated interior surfaces resists corrosion from repeated ethanol or sodium hypochlorite decontamination cycles.

Sample Compatibility & Compliance

The HZQ-F100 accommodates standard laboratory vessels including 250 mL and 500 mL Erlenmeyer flasks, culture tubes, and multi-well plates (with optional adapter trays). Its 26 mm orbit diameter balances mixing efficiency and shear sensitivity—validated for use with mammalian suspension lines (e.g., CHO-K1, HEK293), bacterial cultures (E. coli BL21, Bacillus subtilis), and fungal mycelial suspensions. The unit meets IEC 61010-1 safety requirements for laboratory equipment and complies with electromagnetic compatibility standards EN 61326-1. While not certified for Class II biosafety containment, it is routinely deployed in ISO Class 7 cleanrooms when integrated into laminar airflow hoods or enclosed environmental cabinets.

Software & Data Management

The HZQ-F100 operates as a standalone instrument with embedded firmware—no external PC or proprietary drivers required. All operational parameters are stored in non-volatile memory, retaining settings through power interruption. For laboratories requiring audit trails, optional RS-232 serial output (available via accessory module) enables time-stamped logging of temperature and speed data into LIMS or ELN platforms. When paired with third-party SCADA systems compliant with Modbus RTU protocol, the shaker supports remote start/stop, parameter override, and alarm notification—facilitating alignment with FDA 21 CFR Part 11 electronic record requirements where electronic signatures are implemented at the system level.

Applications

• Microbial growth curve analysis under defined temperature–agitation regimes for strain optimization and bioprocess modeling.
• Protein expression studies using autoinduction media, where precise timing and thermal stability directly impact soluble yield.
• Antibiotic susceptibility testing (AST) via broth microdilution, where uniform mixing prevents sedimentation-induced false resistance calls.
• Enzyme kinetic assays requiring continuous substrate homogenization during spectrophotometric monitoring.
• Preparation of competent cells and phage lysates, where gentle agitation preserves membrane integrity and infectivity titer.
• Environmental microbiology—soil slurry enrichment, bioremediation pilot studies, and anaerobic digester inoculum propagation.

FAQ

Is the HZQ-F100 suitable for use with volatile organic solvents?

Yes—its sealed air-circulation chamber and absence of water reservoirs eliminate vapor condensation pathways; however, solvent compatibility must be verified against gasket materials (silicone elastomer) per ASTM D471.
Can both shelves operate at different temperatures simultaneously?

No—the unit features a single-zone temperature control system; both shelves maintain identical setpoints.
What is the maximum flask fill volume recommended for 250 mL vessels at 280 rpm?

For optimal oxygen transfer and minimal splashing, fill volume should not exceed 30% capacity (i.e., ≤75 mL) at maximum speed.
Does the timer support repeat-cycle programming?

No—the built-in timer is single-cycle only (0–999 minutes); multi-step protocols require external programmable controllers.
Is calibration documentation provided with shipment?

A factory-issued calibration certificate (traceable to NIM, China) is included, covering temperature accuracy and speed verification at three points across the operating range.