Junyi JY300E Electrophoresis Power Supply

Overview

The Junyi JY300E Electrophoresis Power Supply is a microprocessor-controlled, multi-mode DC power source engineered for precise and reproducible electrophoretic separation in molecular biology and protein biochemistry laboratories. Designed around a dual-core embedded control architecture, it delivers stable, low-noise DC output across three independent regulation modes—constant voltage (CV), constant current (CC), and constant power (CP)—enabling optimal experimental flexibility for diverse gel electrophoresis configurations including slab-gel (horizontal and vertical), cellulose acetate membrane, and electroblotting (Western/Northern transfer) applications. Its regulated output maintains ±1% accuracy across the full operating range, minimizing thermal drift and ensuring consistent migration kinetics under variable load conditions. The instrument complies with IEC 61010-1 safety standards for laboratory electrical equipment and incorporates reinforced isolation barriers to meet Class I protection requirements.

Key Features

• Triple-output regulation: Selectable CV, CC, or CP mode with real-time switching and simultaneous display of setpoint and actual values on a backlit LCD.
• High-resolution control: 1 V voltage step, 1 mA current step, and 1 W power step enable fine-tuned method optimization for sensitive nucleic acid or denatured protein separations.
• Advanced programmability: Store up to 100 user-defined electrophoresis protocols; support multi-step sequencing (10 steps per program, up to 10 programs chained) for complex transfer or gradient electrophoresis workflows.
• Intelligent pause functionality: Suspend operation at any time with live parameter adjustment—output stability and regulation fidelity are preserved during resume without recalibration or drift.
• Comprehensive safety architecture: Integrated detection for open-circuit (no-load), short-circuit, overload, and earth leakage conditions; automatic shutdown with audible and visual fault indication.
• Ergonomic human-machine interface: Touch-sensitive membrane keypad with dedicated shortcut keys; non-volatile memory retains settings after power cycle; auto-shutdown after timer expiration or idle timeout.

Sample Compatibility & Compliance

The JY300E is compatible with standard electrophoresis tanks from major manufacturers (e.g., Bio-Rad, Thermo Fisher, C.B.S. Scientific) and supports common gel matrices including polyacrylamide (PAGE, SDS-PAGE, native PAGE), agarose, and cellulose acetate membranes. It meets functional requirements outlined in ISO 13485–aligned quality systems for in vitro diagnostic (IVD) research use and supports GLP-compliant documentation when paired with external logging software. While not FDA-cleared as a medical device, its performance aligns with ASTM D5225 (electrophoretic mobility measurement) and USP guidelines for analytical instrument qualification in pharmaceutical QC labs.

Software & Data Management

The JY300E operates as a standalone unit with no proprietary PC software dependency. All method parameters—including voltage, current, power, time, and volt-hour accumulation—are stored locally in EEPROM with write-cycle endurance exceeding 100,000 cycles. For audit-trail compliance in regulated environments, users may integrate the unit into validated lab networks via optional RS-232 or USB-to-serial adapters (sold separately), enabling timestamped export of run logs to LIMS or ELN platforms. The instrument supports 21 CFR Part 11–compatible electronic signatures when used with validated third-party data acquisition software meeting ALCOA+ principles.

Applications

• Horizontal and vertical SDS-PAGE for protein molecular weight determination and purity assessment.
• Agarose gel electrophoresis for DNA fragment sizing (0.2–20 kb), plasmid analysis, and PCR product verification.
• Electroblotting (wet/tank transfer) of proteins or nucleic acids onto PVDF or nitrocellulose membranes.
• Cellulose acetate electrophoresis for clinical serum protein fractionation (e.g., albumin, α1-, α2-, β-, γ-globulins).
• Two-dimensional electrophoresis (2-DE) first-dimension IEF power supply when configured with low-current, high-voltage ramping profiles.
• Preparative electrophoresis and electroelution where stable, low-ripple DC output is critical for yield and integrity.

FAQ

What electrophoresis tank configurations is the JY300E rated for?
It supports standard horizontal (e.g., mini-submarine) and vertical (e.g., mini-PROTEAN-style) tanks with maximum load impedance up to 10 kΩ at 300 V. Maximum recommended gel area is 20 × 20 cm for agarose and 18 × 16 cm for polyacrylamide.
Does the JY300E support timed shutdown with automatic save of final parameters?
Yes—upon timer expiration, output ceases and the last executed method (including elapsed time, V/I/W, and V·h) is retained in memory for review or reuse.
Can the JY300E be used for isoelectric focusing (IEF)?
It is suitable for passive IEF and basic carrier-ampholyte-based IEF when operated in constant voltage mode with gradual ramping; however, dedicated IEF power supplies with programmable voltage gradients and current limiting are recommended for high-resolution analytical IEF.
Is firmware upgrade capability available?
Firmware updates are performed via RS-232 connection using Junyi’s authorized service tools; end-user field upgrades are not supported to maintain regulatory traceability and calibration integrity.
What safety certifications does the JY300E carry?
It conforms to IEC 61010-1:2010 (Edition 3) for safety of electrical equipment for measurement, control, and laboratory use, including pollution degree 2 and overvoltage category II ratings. CE marking is applied per EU Directive 2014/30/EU (EMC) and 2014/35/EU (LVD).