Junyi JY-SCZF Vertical Electrophoresis Tank

Overview

The Junyi JY-SCZF Vertical Electrophoresis Tank is an engineered platform for high-resolution isoelectric focusing (IEF) and native/polyacrylamide gel electrophoresis (PAGE) of proteins and nucleic acids. Designed around the principles of uniform electric field distribution and thermal stability, it eliminates edge-field distortion through optimized electrode geometry and symmetrical buffer chamber design—critical for reproducible IEF pH gradient formation and sharp band resolution. Its vertical configuration supports slab-gel formats ideal for analytical-scale protein characterization, seed purity testing, and quality control in agricultural biotechnology labs. The system operates without integrated power supply (requires external DC source), enabling precise voltage/current control via certified electrophoresis power supplies compliant with IEC 61010-1 safety standards.

Key Features

• Optimized field homogeneity: Parallel electrode alignment and recessed buffer chambers suppress lateral field dispersion, ensuring linear migration and minimal band distortion—especially critical for IEF-based seed varietal identification.
• Modular thermal management: Integrated ports support connection to external recirculating chillers (e.g., ±0.5 °C stability at 15 °C), mitigating Joule heating during high-voltage runs (up to 3000 V typical for IEF).
• Enhanced operational safety: Mechanical lid-interlock switch cuts power upon opening; insulated safety leads and recessed electrode terminals comply with Class I protection requirements per IEC 61010-1.
• Service-oriented architecture: Removable electrode assemblies allow rapid cleaning, calibration verification, or replacement without disassembling the main tank body—reducing downtime during routine maintenance.
• High-throughput sample processing: Accommodates dual gels with 102-well combs (2 × 102 = 204 samples), supporting parallel analysis for regulatory seed certification per ISTA or AOSA guidelines.

Sample Compatibility & Compliance

The JY-SCZF supports standard acrylamide/bis-acrylamide gels (8–20% T), agarose-IEF hybrid matrices, and immobilized pH gradient (IPG) strips (11 cm format). It is routinely deployed in laboratories performing seed lot purity assessment under national standards (e.g., GB/T 3543.4–1995 in China; equivalent to ISO 20722:2021). When paired with traceable power supplies and calibrated temperature monitors, the system supports data integrity frameworks aligned with FDA 21 CFR Part 11 (electronic records/signatures) and ISO/IEC 17025:2017 clause 7.7 (result reporting). Gel dimensions (306 × 95 mm) conform to common imaging system stage apertures, facilitating downstream UV transillumination or chemiluminescent detection.

Software & Data Management

As a hardware-only electrophoresis platform, the JY-SCZF does not include embedded firmware or proprietary software. It interfaces seamlessly with third-party acquisition tools—including ImageLab (Bio-Rad), AlphaView SA (ProteinSimple), and open-source solutions like Fiji/ImageJ—via standardized gel documentation workflows. Laboratories implementing electronic lab notebooks (ELNs) may log run parameters (voltage, time, cooling setpoint), gel composition, and staining protocols directly into audit-trail-enabled systems. For GLP-compliant environments, metadata capture (operator ID, instrument ID, calibration status) is enforced at the ELN or LIMS level—not within the tank itself—ensuring separation of duties and verifiable chain-of-custody.

Applications

• Seed variety identification and purity testing using esterase or peroxidase zymogram patterns.
• Analytical SDS-PAGE and native PAGE for recombinant protein purity assessment (e.g., post-purification QC per ICH Q5E).
• Two-dimensional electrophoresis (2-DE) first-dimension IEF separations on IPG strips.
• Nucleic acid fragment analysis (e.g., PCR product sizing, RFLP profiling) under denaturing or non-denaturing conditions.
• Method development for electrophoretic mobility shift assays (EMSA) requiring stable, low-drift field conditions.

FAQ

Does the JY-SCZF include a built-in power supply?
No. It is a passive electrophoresis tank requiring an external DC power supply capable of delivering up to 3000 V and 500 mA, with constant voltage or current modes.
Can it be used for SDS-PAGE?
Yes—compatible with standard Laemmli buffer systems and commercial pre-cast or hand-cast polyacrylamide gels (1.0 mm or 1.5 mm thickness).
Is temperature control mandatory for IEF runs?
Strongly recommended. Isoelectric focusing is highly sensitive to thermal gradients; external chiller integration maintains pH gradient integrity and prevents protein denaturation artifacts.
What safety certifications apply to this unit?
The mechanical design meets IEC 61010-1:2010 requirements for laboratory electrical equipment; final compliance depends on integration with certified power supplies and proper grounding per local regulations.
How is calibration verified?
Electrode spacing and buffer volume are factory-dimensioned and mechanically fixed; users verify field uniformity via test runs with dye markers (e.g., bromophenol blue/xylene cyanol) and assess band straightness visually or quantitatively using densitometry software.