Laballiance Series III Constant Flow HPLC Pump
| Brand | Laballiance |
|---|---|
| Origin | USA |
| Model | Series III |
| Flow Rate Ranges | 0.001–5 mL/min, 0.01–10 mL/min, 0.1–40 mL/min |
| Maximum Pressure | 6,000 psi (for 5 & 10 mL heads), 1,500 psi (for 40 mL head) |
| Flow Accuracy | ±1% |
| Flow Precision | ≤0.075% RSD |
| Dimensions | 5.5" H × 10.4" W × 17.5" D |
| Weight | 24 lb (11 kg) |
| Power Supply | 220 V, 50/60 Hz (or 110/120 V variants available) |
| Key Features | Integrated pressure sensor with LED display, self-cleaning pump head, patented low-volume diaphragm pulse damper, dual check valve design, RS-232 interface, 0–10 kHz frequency input (0.01–9.99 mL/min), 0–10 V start/stop control |
Overview
The Laballiance Series III Constant Flow HPLC Pump is an engineered solution for high-stability, low-pulsation liquid delivery in analytical and semi-preparative chromatographic systems. Built upon the proven architecture of the Series II platform, the Series III introduces critical enhancements—including a rapid-fill cam-driven suction mechanism and a proprietary low-volume diaphragm pulse damper—that collectively reduce baseline noise and flow pulsation to levels compatible with sensitive UV, fluorescence, and mass spectrometric detection. Its operation follows positive displacement principles using dual-sapphire or ceramic check valves and chemically resistant pump heads (stainless steel or PEEK), ensuring bidirectional flow integrity and minimal backflow across extended run times. Designed for continuous duty in regulated environments, the Series III meets foundational requirements for method robustness in QC laboratories, pharmaceutical development, and academic research where reproducible solvent delivery under variable backpressure is essential.
Key Features
- Integrated high-resolution pressure transducer with real-time LED readout—enabling immediate system diagnostics and overpressure protection
- Self-cleaning pump head architecture with tool-free maintenance access, reducing downtime and consumable replacement costs
- Dual non-return valve configuration (sapphire or ceramic) for zero backflow and long-term seal integrity
- Digitally controlled stepper motor drive with precision microstepping for stable flow control across full dynamic range
- Modular flow path options: stainless steel (SS) and bio-inert PEEK configurations, supporting compatibility with aggressive solvents and biomolecules
- Front-panel interface with five dedicated function keys—streamlining flow rate entry, pressure limit setting, start/stop, quick flush, and parameter selection
- RS-232 serial port and analog I/O (0–10 V start/stop, 0–10 kHz frequency input) for integration into automated workflows and LIMS-controlled environments
Sample Compatibility & Compliance
The Series III accommodates a broad range of mobile phases—from aqueous buffers and organic solvents (acetonitrile, methanol, THF) to corrosive acids and bases—when equipped with PEEK or titanium-wetted components. Its construction adheres to standard mechanical safety practices for laboratory instrumentation (IEC 61010-1). While not certified as a medical device, the pump supports GLP- and GMP-aligned workflows through traceable pressure monitoring, audit-ready operational logs (when paired with compliant software), and configurable alarm thresholds for pressure excursions. It is routinely deployed in applications requiring compliance with USP , ASTM E260, and ISO 13877 for liquid chromatography system performance verification.
Software & Data Management
Though standalone in operation, the Series III interfaces seamlessly with third-party chromatography data systems (CDS) via RS-232 or analog signals. Its pressure and flow status can be logged externally for trend analysis and preventive maintenance scheduling. When integrated into validated environments, the pump’s digital inputs support 21 CFR Part 11–compliant electronic signatures when used with appropriate CDS platforms that enforce user authentication, audit trails, and electronic record retention. Firmware updates are performed offline via serial connection, preserving system integrity during qualification.
Applications
- Analytical-scale isocratic and binary gradient HPLC (e.g., small-molecule purity assessment, stability-indicating assays)
- Semi-preparative purification (up to 40 mL/min) for natural product isolation and synthetic intermediate recovery
- Biochromatography requiring biocompatible fluid paths (e.g., protein A affinity, ion exchange, size exclusion)
- Method development and transfer labs where flow consistency directly impacts retention time reproducibility
- Academic teaching laboratories—leveraging intuitive controls and visual feedback for chromatography fundamentals instruction
- Quality control testing per pharmacopeial monographs where pressure monitoring validates column health and system integrity
FAQ
What flow rate ranges are supported by the Series III pump?
The Series III is available in three calibrated flow modules: 0.001–5 mL/min (high-precision analytical), 0.01–10 mL/min (general-purpose), and 0.1–40 mL/min (semi-preparative), each optimized for corresponding pressure ratings.
Does the pump support gradient elution?
The Series III is a constant-flow isocratic pump; however, it may serve as one channel in a dual-pump gradient system when paired with a second Series III unit and external mixing tee or low-dead-volume combiner.
How is pump head cleaning automated?
The self-cleaning cycle uses reverse-flow actuation during idle periods or manual initiation, flushing residual solvent from check valves and piston seals without disassembly.
Can the Series III be operated remotely?
Yes—via RS-232 command set or analog voltage/frequency inputs, enabling synchronization with autosamplers, detectors, or PLC-based process controllers.
What materials are available for wetted parts?
Stainless steel (316 SS) and PEEK are standard options; custom configurations with titanium or Hastelloy components are available upon request for specialized corrosion resistance.
