MENTS SPE-12P Solid Phase Extraction System

Overview

The MENTS SPE-12P Solid Phase Extraction System is a semi-automatic, 12-position parallel vacuum manifold engineered for reproducible, high-throughput sample preparation in analytical laboratories. It implements classical solid-phase extraction (SPE) methodology—based on selective adsorption, washing, and elution of analytes onto solid sorbent cartridges—to isolate, purify, and concentrate target compounds from complex liquid matrices (e.g., environmental water, biological fluids, food extracts, and pharmaceutical formulations). Unlike manual syringe-driven or gravity-fed systems, the SPE-12P integrates a precision-regulated vacuum manifold with chemically inert PTFE fluid pathways and a robust borosilicate glass vacuum chamber, enabling consistent flow control across all 12 channels without cross-contamination. Its design adheres to fundamental principles of interfacial chemistry and sorbent–analyte affinity, supporting method development aligned with ISO 17994, ASTM D5673, and USP chromatographic sample preparation guidelines.

Key Features

• 12-channel parallel configuration with independent flow control per position—enabling simultaneous processing of up to 12 samples while maintaining method integrity.
• Vacuum manifold constructed from molded borosilicate glass with uniform wall thickness, rated for sustained operation at –80 kPa; recommended operational range is 0 to –65 kPa for optimal cartridge packing and elution kinetics.
• PTFE-lined fluid path throughout—including vacuum tubing, solvent delivery lines, and cartridge adapters—ensuring compatibility with aggressive solvents (e.g., acetonitrile, methanol, dichloromethane, 0.1% formic acid, 1M NaOH).
• Chemically resistant polymer tray and lid (imported high-performance polymer), resistant to strong acids, bases, and organic solvents—validated for long-term use under continuous vacuum stress.
• Included universal cartridge adapter set supports standard 1 mL, 3 mL, and 6 mL SPE columns; enables direct activation, equilibration, and elution using collection tubes without column transfer.
• Integrated pressure regulation valve with PTFE-composite sealing elements—provides stable vacuum control, thermal stability from –20 °C to +120 °C, and corrosion resistance over extended service life.
• Ergonomic, space-efficient footprint (240 × 80 × 175 mm) optimized for benchtop integration within regulated QC/QA environments.

Sample Compatibility & Compliance

The SPE-12P accommodates aqueous, buffered, and mildly organic sample matrices—including plasma, urine, surface water, wastewater, beverage extracts, and plant tissue homogenates. Its PTFE-wetted architecture ensures compliance with GLP and GMP requirements for trace-level analysis, minimizing analyte loss and matrix carryover. The system supports validated SPE methods compliant with EPA Method 502.2 (halogenated organics), EPA Method 549.2 (pesticides), and ISO 11357-4 (polymer extractables). All structural components are non-leaching and free of detectable heavy metals or plasticizers, satisfying ICH Q5C and USP material safety criteria for pharmaceutical sample prep.

Software & Data Management

As a semi-automatic platform, the SPE-12P does not incorporate embedded firmware or digital control interfaces. However, it is fully compatible with laboratory documentation systems requiring manual entry into electronic lab notebooks (ELNs) or LIMS. Vacuum pressure settings, solvent sequence, and sample tracking are recorded per SOP using standardized log sheets—supporting full audit trails required under FDA 21 CFR Part 11 when paired with compliant ELN software (e.g., LabArchives, Benchling). Optional calibration logs for vacuum gauge verification are included in the user manual to support ISO/IEC 17025 accreditation.

Applications

• Environmental analysis: Pre-concentration of PAHs, PCBs, and pharmaceutical residues from drinking water and wastewater prior to GC-MS or LC-MS/MS.
• Clinical toxicology: Cleanup and enrichment of opioids, benzodiazepines, and stimulants from serum and urine for quantitative immunoassay or mass spectrometry.
• Food safety testing: Isolation of mycotoxins (e.g., aflatoxin B1), veterinary drug residues (e.g., sulfonamides), and pesticide metabolites from milk, honey, and fruit extracts.
• Pharmaceutical R&D: Desalting and buffer exchange of peptide digests; removal of phospholipids from plasma prior to bioanalysis.
• Academic research: Method development for novel sorbents (e.g., molecularly imprinted polymers, graphene oxide composites) under controlled flow conditions.

FAQ

Is the SPE-12P compatible with 1 mL, 3 mL, and 6 mL SPE cartridges?

Yes—the system includes universal PTFE adapters designed for secure, leak-free mounting of all standard cartridge sizes.
Can the vacuum pressure be adjusted during a run?

Yes—via the integrated PTFE composite pressure regulator, allowing real-time adjustment between 0 and –65 kPa.
What solvents are compatible with the wetted materials?

All common SPE solvents including methanol, acetonitrile, ethyl acetate, dichloromethane, hexane, 0.1% formic acid, 0.1% ammonium hydroxide, and 1M NaOH.
Does the system require external vacuum source?

Yes—a laboratory vacuum pump (oil-free recommended) or central vacuum line is required; no built-in pump is included.
Are replacement parts available for long-term maintenance?

Yes—PTFE tubing, glass manifolds, polymer trays, and cartridge adapters are stocked and supplied with full part numbers for traceable procurement.