HUXI HT-W330 Orbital Reciprocating Shaker

Overview

The HUXI HT-W330 Orbital Reciprocating Shaker is an engineered platform for precise, reproducible mixing of biological and biochemical samples under controlled ambient or low-temperature conditions. Unlike rotary or linear shakers, the HT-W330 employs a true orbital reciprocating motion—combining horizontal orbital displacement with synchronized back-and-forth oscillation—to generate uniform shear forces across sample vessels. This dual-mode agitation enhances mass transfer efficiency in applications such as gel fixation, staining, destaining, and silver nitrate-based development protocols. Designed for routine use in molecular biology labs, core facilities, and QC environments, the unit integrates a brushless DC motor for maintenance-free operation, microprocessor-based speed and time regulation, and passive thermal management to sustain stable chamber temperatures between 5 °C and 40 °C without active refrigeration or heating elements.

Key Features

• Brushless DC motor ensures long service life, minimal electromagnetic interference, and consistent torque delivery across the full 60–500 rpm range.
• Reciprocating orbital drive mechanism delivers uniform energy distribution—critical for reproducible electrophoretic gel processing and membrane-based assays.
• LCD digital display provides real-time feedback on setpoint and actual speed, elapsed time, and remaining timer duration.
• Precision timer supports programmable intervals from 1 minute up to 99 hours and 59 minutes, with audible end-of-cycle alarm and automatic shutdown.
• Compact footprint (330 × 370 × 130 mm) and IP21-rated enclosure enable safe placement in biosafety cabinets, cold rooms, or shared benchtop spaces.
• Universal voltage input (100–240 V AC, 50/60 Hz) guarantees operational stability across global laboratory power infrastructures.
• 4 mm stroke amplitude balances gentle suspension of sensitive cells with robust mixing of viscous solutions—optimized for standard microtiter plates, Petri dishes, and glass staining trays.

Sample Compatibility & Compliance

The HT-W330 accommodates standard labware including 6–96-well microplates, rectangular staining dishes (up to 200 × 250 mm), conical tubes (50 mL), and Erlenmeyer flasks (up to 250 mL) via interchangeable clamping accessories (sold separately). Its mechanical design complies with IEC 61010-1:2010 for electrical safety in laboratory equipment. While not certified for GMP or GLP environments out-of-the-box, the shaker’s deterministic timing logic, non-volatile parameter retention, and RS232 serial interface (optional) support integration into validated workflows requiring audit trails—particularly when paired with external data loggers compliant with FDA 21 CFR Part 11 requirements.

Software & Data Management

The HT-W330 operates autonomously via its embedded firmware; no host PC or proprietary software is required for basic operation. Optional RS232 connectivity enables remote command execution (e.g., START, STOP, SET SPEED, QUERY STATUS) and timestamped event logging using third-party terminal emulators or LIMS-integrated control modules. All user-defined parameters—including speed, duration, and temperature setpoints—are retained in non-volatile memory after power cycling, ensuring protocol consistency across shifts. For laboratories implementing electronic recordkeeping, the device’s deterministic behavior and absence of internal storage align with ALCOA+ principles when used within documented SOPs.

Applications

• Electrophoresis gel processing: Coomassie Blue fixation, destaining, and silver nitrate staining/development cycles.
• Suspension culture of adherent cell lines during passaging or transfection preparation.
• Antibody incubation and blocking steps in Western blotting and ELISA workflows.
• Extraction and homogenization of tissue lysates in low-viscosity buffers.
• Hybridization buffer equilibration for DNA/RNA blotting procedures.
• Quality control testing of reagent stability and lot-to-lot consistency under defined agitation profiles.

FAQ

Does the HT-W330 include temperature control hardware?
No—the unit maintains ambient temperature only. The 5–40 °C specification refers to the permitted operating environment, not active thermoregulation.
Can the shaker accommodate deep-well plates or orbital flasks?
Yes, with optional universal platform clamps or custom-fit adapters (part numbers available upon request).
Is speed calibration traceable to national standards?
Factory calibration is performed using NIST-traceable tachometric reference instruments; certificate of conformance is provided with each unit.
What maintenance is required for the brushless DC motor?
None—no brushes to replace, no lubrication points, and no scheduled servicing over its rated lifetime (>20,000 operating hours).
Is the RS232 interface optically isolated?
Yes—the optional RS232 module includes galvanic isolation to prevent ground-loop interference in multi-instrument networks.