Orient KOJI OKI-uFRLs Integrated Micro-Raman & Fluorescence Lifetime Imaging System
| Brand | Orient KOJI |
|---|---|
| Origin | Tianjin, China |
| Manufacturer Type | Authorized Distributor |
| Country of Origin | China |
| Model | OKI-uFRLs |
| Price Range | USD 210,000 – 280,000 (FOB Tianjin) |
| Spatial Resolution | < 1 µm |
| Spectral Resolution | ≤ 1 cm⁻¹ |
| Spectral & Lifetime Wavelength Range | 250 – 1650 nm |
| Fluorescence Lifetime Range | 100 ps – 10 ms |
| Maximum Coupled Raman Excitation Lasers | 4 wavelengths |
| Maximum FLIM Excitation Lasers | 4 wavelengths |
| Shortest Excitation Wavelength | 266 nm |
| Detector Types | High-Sensitivity PMT + Cooled CCD |
| Scanning Axes | XYZ motorized stage |
| Optical Zoom | 0.3× – 1.5× |
| Microscope Configuration | Upright or Inverted |
| Illumination Source | Tungsten-Halogen Lamp |
| Image Workstation | Integrated Windows-based platform with real-time acquisition & analysis suite |
| Vibration Isolation Table | 1500 × 1800 mm dedicated optical-grade active/passive hybrid isolation platform |
| Temperature Range (with optional cryo/hot stages) | 4 K – 550 K |
| Pressure Range (with optional DAC modules) | Up to 100 GPa (DAC) / up to 100 MPa (hydrostatic cell) |
Overview
The Orient KOJI OKI-uFRLs Integrated Micro-Raman & Fluorescence Lifetime Imaging System is a research-grade multimodal optical platform engineered for correlative microspectroscopic analysis at submicron spatial resolution. It combines confocal Raman spectroscopy, time-resolved fluorescence lifetime imaging (FLIM/PLIM), micro-transmission/reflection absorbance mapping, upconversion luminescence spectroscopy, and synchronized spectral–temporal acquisition within a single, modular microscope architecture. The system operates on the principle of laser-induced inelastic scattering (Raman) and time-correlated single-photon counting (TCSPC) for fluorescence decay analysis, enabling quantitative molecular fingerprinting and dynamic electronic-state characterization across solid-state materials, biological tissues, thin-film devices, and quantum emitters. Its dual-excitation capability—supporting up to four independently tunable Raman lasers and four pulsed FLIM excitation sources—ensures broad spectral coverage from deep-UV (266 nm) to near-infrared (1650 nm), while maintaining full spectral resolution ≤1 cm⁻¹ and temporal resolution down to 100 ps.
Key Features
- Submicron spatial resolution (<1 µm) achieved via diffraction-limited confocal optics and high-NA objective integration
- Simultaneous or sequential acquisition of Raman spectra, fluorescence intensity maps, lifetime decay histograms, and spectral-temporal cross-correlations
- Dual-detector architecture: ultra-low-noise photomultiplier tube (PMT) for TCSPC-based FLIM and thermoelectrically cooled scientific CCD for high-dynamic-range Raman spectral imaging
- Motorized XYZ scanning stage with programmable trajectory control and sub-100 nm step precision
- Continuously variable optical zoom (0.3×–1.5×) enabling seamless transition between wide-field survey and high-magnification microanalysis
- Modular microscope base supporting both upright and inverted configurations, compatible with standard Nikon, Olympus, or Zeiss objective mounts
- Integrated halogen-tungsten broadband illumination for brightfield/darkfield/phase contrast correlation with spectroscopic data
- Dedicated 1500 × 1800 mm hybrid vibration isolation table optimized for long-integration spectroscopic measurements and low-signal TCSPC acquisition
Sample Compatibility & Compliance
The OKI-uFRLs accommodates diverse sample geometries—from bulk crystals and polished wafers to live-cell monolayers and frozen-hydrated tissue sections—within ambient, inert gas, vacuum, or controlled environmental chambers. Optional cryogenic stages support operation from 4 K to 550 K, while diamond anvil cell (DAC) and hydrostatic pressure modules extend mechanical testing capabilities up to 100 GPa and 100 MPa respectively. All hardware and software components comply with ISO/IEC 17025 requirements for analytical instrument qualification. Data acquisition workflows adhere to GLP and GMP principles where applicable; audit trails, user access logs, and electronic signature support are embedded in the acquisition software to facilitate FDA 21 CFR Part 11 compliance in regulated environments.
Software & Data Management
The system is controlled by a dedicated Windows-based workstation running Orient KOJI’s uFRLs Acquisition Suite—a modular application built on LabVIEW and Python backends. It provides real-time spectral preview, lifetime decay fitting (multi-exponential, stretched exponential, phasor analysis), Raman peak deconvolution (Voigt/Gaussian/Lorentzian), and pixel-wise parametric mapping (τ₁, τ₂, amplitude ratios, spectral centroid shifts). Raw data is stored in vendor-neutral HDF5 format with embedded metadata (laser power, integration time, grating position, detector gain, temperature, pressure). Batch processing pipelines support automated calibration correction (wavelength, intensity, lifetime offset), inter-channel registration, and export to common formats including .csv, .tif, .fli, and .spc for third-party analysis in MATLAB, Origin, or ImageJ/Fiji.
Applications
- Characterization of 2D materials (graphene, TMDCs, hBN) via strain mapping, layer-number identification, defect density quantification, and exciton lifetime dynamics
- Pharmaceutical solid-state analysis: polymorph distribution, crystallinity gradients, API-excipient interactions, and degradation kinetics under thermal stress
- Microplastics identification and aging assessment using Raman fingerprint libraries coupled with FLIM-based oxidation state mapping
- Photovoltaic device metrology: perovskite phase segregation, interfacial recombination lifetimes, and carrier diffusion length estimation
- Biomedical research: label-free cellular metabolism monitoring (NADH/FAD redox ratio), amyloid aggregation kinetics, and nanoparticle biodistribution via upconversion luminescence lifetime tracking
- Geoscience and high-pressure physics: phase transitions in mantle minerals, vibrational mode softening under compression, and non-radiative relaxation pathways in shocked ceramics
FAQ
Can the system perform simultaneous Raman and FLIM acquisition?
Yes—the OKI-uFRLs supports hardware-synchronized dual-channel detection using time-gated spectral separation or wavelength-splitting dichroics, enabling concurrent collection without signal crosstalk.
Is the software compatible with third-party analysis tools?
All raw and processed datasets are saved in open HDF5 format with standardized metadata schemas, ensuring direct import into MATLAB, Python (h5py, scikit-image), and commercial packages such as Igor Pro and GRAMS/AI.
What level of service and calibration support is provided?
Orient KOJI offers factory calibration certificates traceable to NIST standards for spectral axis, intensity linearity, and timing accuracy. On-site installation, operator training, and annual performance verification are available under extended service agreements.
How is laser safety managed during multi-wavelength operation?
The system integrates interlocked beam shutters, real-time power monitoring, and Class 1 enclosure certification per IEC 60825-1:2014. All laser paths are fully enclosed and accessible only via safety-rated service panels.
Can the platform be upgraded for transient absorption or second-harmonic generation?
Yes—its modular optical layout includes reserved beam ports, kinematic mirror mounts, and synchronization I/O (TTL, LVDS) to integrate pump-probe delay stages, OPOs, or nonlinear crystal modules for future expansion into ultrafast spectroscopy modalities.
