Omnifit EZ SolventPlus Axial Compression Glass Chromatography Column (Sanotac OEM, UK-Made)

Overview

The Omnifit EZ SolventPlus Axial Compression Glass Chromatography Column is a high-integrity, chemically inert preparative chromatography column engineered for medium-pressure liquid chromatography (MPLC), protein purification, and synthetic chemistry applications. Designed and manufactured in the United Kingdom by Omnipore (distributed globally under the Omnifit® brand), this column employs a robust borosilicate glass tube construction coupled with precision-machined inert polymer components—including PEEK™ end fittings, Chemraz® elastomeric seals, PTFE frits, and ETFE compression hardware—to ensure full compatibility with aggressive organic solvents and stringent aqueous mobile phases. Its axial compression architecture—available in one-end adjustable (AF) and dual-end adjustable (AA) configurations—enables precise bed consolidation during column packing and operation, minimizing channeling and maximizing resolution across diverse stationary phases (e.g., silica gel, reversed-phase C18/C4, ion exchange resins, and affinity media). The column is fully compatible with major automated protein purification platforms including Cytiva AKTA systems, Sanotac Biolot systems, and Bio-Rad NGC chromatography systems, supporting both gravity-fed and pump-driven workflows up to 10 bar (150 psi) at 25 mm internal diameter.

Key Features

• Borosilicate glass column body with certified thermal and mechanical stability (operating range: 4–40 °C)
• Full inert fluid path: no metal contact; all wetted surfaces consist of borosilicate glass, PTFE, Chemraz®, PEEK™, and ETFE
• Chemical resistance validated for pH 1–14 and broad solvent classes including strong acids/bases, polar aprotic solvents (DMSO, DMF), halogenated organics (chloroform, DCE), and aromatic hydrocarbons (toluene)
• Modular axial compression design enables reproducible bed density control—critical for method transfer and scale-up from analytical to preparative formats
• Standardized 1/4″-28 UNF threaded connections facilitate rapid integration with standard HPLC tubing, unions, and system manifolds
• Available in six inner diameters (5–50 mm) and column lengths from 50–500 mm, supporting volumes from <1 mL to 940 mL
• Omnifit EZ SolventPlus variant optimized specifically for solvent-intensive applications such as reverse-phase preparative LC and continuous-flow synthesis

Sample Compatibility & Compliance

This column supports a wide range of biomolecular and small-molecule separation workflows. It is routinely employed in GMP-aligned protein purification processes where extractables and leachables must be minimized—its non-metallic construction eliminates metal ion contamination risks associated with stainless-steel columns. The Chemraz® O-rings meet USP Class VI biocompatibility standards, and PTFE frits conform to FDA 21 CFR §177.1550 for food-contact polymers. While not inherently GLP/GMP-certified as a standalone device, the column’s design supports audit-ready workflows when used within validated systems (e.g., AKTA Pure with UNICORN software configured for 21 CFR Part 11 compliance). It conforms to ISO 8573-1 for compressed air purity when used with pneumatic compression accessories, and its pressure ratings align with ASTM D3350 (plastic pipe materials) and ISO 7208 (chromatography column performance testing guidelines).

Software & Data Management

As a passive hardware component, the Omnifit EZ SolventPlus column does not incorporate embedded electronics or firmware. However, it integrates natively with industry-standard chromatography data systems (CDS) via analog pressure transducers or digital flow sensors installed upstream/downstream in the system manifold. When deployed on Cytiva AKTA platforms, column performance parameters—including backpressure trends, flow consistency, and fraction collection timing—are logged automatically within UNICORN software, enabling full traceability per ICH Q5A and EU Annex 11 requirements. Sanotac Biolot systems support CSV export of operational logs for external LIMS ingestion. All physical configuration data (ID, length, compression setting, packing date) should be manually recorded in electronic lab notebooks (ELN) to maintain chain-of-custody integrity during regulatory submissions.

Applications

• Preparative purification of monoclonal antibodies, recombinant enzymes, and fusion proteins using ion exchange, size exclusion, or affinity chromatography
• Isolation of natural products from botanical extracts (e.g., traditional Chinese medicine fractions) under GRAS-compliant solvent conditions
• Method development and scale-up studies bridging analytical HPLC to pilot-scale MPLC
• Continuous-flow synthesis workups requiring inline quenching and scavenger-column cleanup
• Stability-indicating assays for biopharmaceutical aggregates and deamidation variants
• Custom-packed columns for chiral separations using polysaccharide-based CSPs

FAQ

What is the maximum operating pressure for the 25 mm ID column?
The 006EZS-25-50-AF column is rated for continuous operation at up to 10 bar (150 psi) at 25 mm internal diameter, as specified in the Omnifit EZ SolventPlus pressure rating table.
Can this column be sterilized using autoclaving?
No. Borosilicate glass is heat-resistant, but Chemraz® O-rings and ETFE components are not autoclave-stable. Sterilization must be performed via 0.22 µm filtration of solvents or chemical sanitization with ethanol/water or dilute sodium hydroxide.
Is axial compression necessary for all applications?
Axial compression is recommended for any application involving dry-packed or slurry-packed beds, especially with compressible media (e.g., agarose-based resins), to prevent bed settling and void formation during extended runs.
How do I select between AF (one-end adjustable) and AA (dual-end adjustable) configurations?
AF columns are optimal for routine protein purification where consistent bed height and moderate pressure stability suffice; AA columns provide superior control for high-resolution small-molecule separations requiring fine-tuned bed density gradients.
Are replacement parts available separately?
Yes. Sanotac supplies OEM-certified replacement kits including Chemraz® O-rings (P/N OR-EZ-25), PTFE frits (P/N FR-EZ-25), and ETFE compression nuts (P/N CN-EZ-25), all traceably documented per batch.