Sanotac HPLC-5C18E Semi-Preparative/Preparative Reversed-Phase Chromatography Column
| Brand | Sanotac |
|---|---|
| Origin | Shanghai, China |
| Manufacturer Type | Direct Manufacturer |
| Regional Classification | Domestic (China) |
| Model | HPLC-5C18E |
| Application Level | Laboratory-Grade |
| Instrument Type | High-Pressure Preparative Liquid Chromatography System |
| Flow Rate Range | 0.01–100.00 mL/min |
| Flow Accuracy | ±1% |
| Flow Precision (RSD) | ≤0.5% |
| Maximum Operating Pressure | 10 MPa |
| Wavelength Range | ±1 nm |
| Wavelength Repeatability | 0.2 nm |
| Baseline Noise | ±0.5 × 10⁻⁵ AU (254 nm, TC = 1 s) |
| Data Acquisition Frequency | 10 Hz |
Overview
The Sanotac HPLC-5C18E is a semi-preparative to preparative-scale reversed-phase chromatography column engineered for high-resolution, high-loading purification of small molecules, natural products, synthetic intermediates, and early-stage pharmaceutical compounds. Designed for integration into high-pressure preparative liquid chromatography (PLC) systems, it operates under controlled isocratic or gradient elution conditions with precise flow delivery and UV detection at 254 nm. The column utilizes endcapped C18 silica-based packing material with 5 µm particle size and fully porous morphology, offering robust chemical stability across pH 2–8 and exceptional batch-to-batch reproducibility—critical for method transfer from analytical to preparative scale. Its mechanical integrity supports sustained operation at up to 10 MPa (1450 psi), enabling efficient separation of moderately polar to nonpolar analytes while maintaining peak symmetry and minimal backpressure drift over extended runs.
Key Features
- Pre-packed with premium-grade, imported C18 stationary phase (5 µm, 120 Å pore size), ensuring high surface area and consistent retention behavior
- Optimized column geometry for scalable purification: available in inner diameters of 10 mm, 20 mm, 30 mm, and 50 mm—with corresponding load capacities ranging from 12 mg to 300 mg per injection
- Robust stainless-steel housing with zero-dead-volume frits and precision-machined end-fittings compatible with standard 1/4″-28 UNF or M6 × 1 connectors
- Validated flow performance: RSD ≤ 0.5% for retention time and peak area under repeated injections (n ≥ 6), meeting ICH Q2(R2) intermediate precision criteria
- Extended column lifetime due to rigorous slurry packing uniformity, minimized channeling, and resistance to silanol activity-induced tailing
- Wide pH compatibility (pH 2–8) supported by high-purity silica and stable bonding chemistry—enabling method development flexibility without column degradation
Sample Compatibility & Compliance
The HPLC-5C18E column is validated for use with common organic modifiers (acetonitrile, methanol), aqueous buffers (ammonium acetate, ammonium formate, phosphate), and low-concentration TFA or formic acid additives. It complies with ISO 17025 requirements for calibration traceability when used within certified preparative LC workflows. Column performance data—including plate count (N > 25,000/m for caffeine at 1 mL/min), asymmetry factor (As = 0.9–1.2), and resolution (Rs ≥ 1.5 for critical pairs)—are documented per batch and supplied with CoA. For regulated environments, the column supports GLP/GMP-compliant purification protocols when paired with 21 CFR Part 11-enabled chromatography data systems (CDS) and audit-trail-capable acquisition software.
Software & Data Management
While the column itself is hardware-only, its performance is fully leveraged through modern preparative CDS platforms such as Waters Empower, Shimadzu LabSolutions Prep, or Thermo Chromeleon. These systems support automated fraction collection triggered by UV threshold, time window, or peak apex detection—all synchronized at 10 Hz sampling rate to capture transient elution profiles accurately. Baseline noise specification (±0.5 × 10⁻⁵ AU at 254 nm, 1 s time constant) ensures reliable integration of low-abundance components in complex matrices. All raw chromatograms, method parameters, and fraction metadata are stored in vendor-neutral formats (e.g., .cdf, .wiff) compliant with laboratory informatics infrastructure (LIMS/ELN).
Applications
- Purification of synthetic APIs and chiral intermediates prior to crystallization or biological testing
- Isolation of bioactive constituents from plant extracts, fermentation broths, or combinatorial libraries
- Desalting and buffer exchange of peptide fractions post-solid-phase synthesis
- Removal of residual catalysts or protecting groups in multi-step organic synthesis workflows
- Method scouting and scale-up studies bridging analytical HPLC (4.6 mm ID) to preparative throughput (≥20 mm ID)
- Supporting quality control of reference standards per USP and EP 2.2.46 chromatographic separations
FAQ
What is the recommended equilibration protocol before first use?
Condition with 10 column volumes of mobile phase at 1 mL/min (for 10 mm ID), followed by 5 CV at operating flow rate; monitor baseline stability and pressure profile.
Can this column be used with acidic or basic mobile phases?
Yes—validated for pH 2–8; avoid prolonged exposure below pH 2 or above pH 8 to preserve silica integrity and bonding stability.
How often should the column be cleaned between batches?
Perform a regeneration wash with 10 CV of 100% acetonitrile or isopropanol after each run; for strongly retained contaminants, add 5 CV of 5% DCM in IPA (if system-compatible).
Is column performance data traceable to NIST standards?
Batch-specific test chromatograms and efficiency metrics are traceable to internal reference standards calibrated against USP Lycopodium Clavatum spore suspension and caffeine CRMs.
Does Sanotac provide column recharging or refurbishment services?
Yes—Sanotac offers authorized column repacking and end-fitting replacement under ISO 9001-certified service protocols, with full QC documentation upon return.
