Omnifit EZS-06-25-AF High-Pressure Axial Compression Chromatography Column (Borosilicate Glass, PEEK & Chemraz® Compatible)
| Origin | UK |
|---|---|
| Manufacturer Type | Authorized Distributor |
| Product Origin | Imported |
| Model | 006EZS-10-25-AF |
| Application Level | Laboratory Grade |
| Instrument Type | Medium-Low Pressure Preparative Liquid Chromatography |
| Max Operating Pressure | 900 psi (60 bar) for 6.6 mm ID |
| Column Material | Borosilicate Glass Tube, PTFE Frits, Chemraz® O-Rings, ETFE End Fittings |
| Temperature Range | 4–40 °C |
| pH Stability | 1–14 |
| Chemical Compatibility | Compatible with aqueous buffers, acetonitrile, methanol, THF, DMSO, DMF, chloroform, dichloroethane, pyridine, formamide, toluene |
Overview
The Omnifit EZS-06-25-AF is a high-performance axial compression chromatography column engineered for medium-pressure preparative liquid chromatography (PLC) applications in academic, biopharmaceutical, and analytical laboratories. Designed and manufactured in the United Kingdom by Omnipore (trading as Omnifit), this column employs a precision-engineered borosilicate glass body combined with chemically inert fluidic path components—including PTFE frits, Chemraz® elastomeric seals, and ETFE end fittings—to ensure exceptional chemical resistance and mechanical stability under sustained pressure. Unlike fixed-bed columns, the axial compression configuration allows dynamic bed consolidation via adjustable end fittings, minimizing channeling and enhancing packing homogeneity—critical for reproducible resolution of proteins, peptides, natural products, and synthetic intermediates. Its operational pressure envelope (up to 900 psi for the 6.6 mm internal diameter variant) bridges the gap between low-pressure flash chromatography and high-pressure HPLC systems, making it compatible with widely deployed preparative platforms including Cytiva AKTA systems, Bio-Rad NGC, and Sanotac Biolot purification workstations.
Key Features
- Borosilicate glass column body offering optical clarity, thermal stability (4–40 °C), and broad chemical compatibility across pH 1–14.
- Axial compression design with dual-adjustable end caps enables precise control over bed density and uniformity—essential for maintaining column efficiency during repeated loading cycles.
- Chemically inert wetted materials: PTFE frits (10 µm pore size standard), Chemraz® O-rings (superior solvent resistance vs. Viton® or silicone), and ETFE compression nuts eliminate metal ion leaching and adsorptive artifacts.
- Modular scalability: Available in six internal diameters (6.6, 10, 15, 25, 35, 50 mm) and lengths from 50 mm to 500 mm—supporting sample loads from milligram to multi-gram scale.
- Validated performance with common organic modifiers (e.g., acetonitrile, methanol, THF, DMSO) and aggressive solvents including chloroform, pyridine, and DMF—enabling both reversed-phase and normal-phase purification protocols.
- Designed for rapid assembly/disassembly without tools; standardized threading (¼-28 UNF) ensures interchangeability with standard PLC manifolds and fraction collectors.
Sample Compatibility & Compliance
The EZS-06-25-AF supports diverse biomolecular and small-molecule purification workflows, including recombinant protein capture (e.g., His-tagged elution), monoclonal antibody polishing, oligonucleotide desalting, and chiral separation of pharmaceutical intermediates. Its construction complies with ISO 8573-1 (compressed air purity) for pneumatic actuation interfaces and meets material safety requirements per USP for polymeric components. While not certified for GMP manufacturing, the column’s traceable batch documentation, absence of extractables (verified per USP ), and compatibility with GLP-compliant data acquisition systems make it suitable for method development, QC release testing, and preclinical batch purification under regulated environments. It is routinely employed in workflows aligned with ICH Q5A (biological product characterization) and ASTM D8022 (chromatographic column qualification).
Software & Data Management
As a hardware component—not an instrument—the EZS-06-25-AF integrates transparently into existing chromatography data systems (CDS) such as UNICORN (Cytiva), Bio-Rad Director, or third-party ELN/LIMS platforms via analog pressure/UV output signals from upstream detectors and pumps. Column metadata (ID, dimensions, packing date, solvent history) can be manually logged or auto-populated using QR-coded labels (optional accessory). For audit-trail integrity, users are advised to record column usage parameters—including flow rate, backpressure trends, and regeneration cycles—in accordance with FDA 21 CFR Part 11–compliant electronic notebooks when deployed in regulated settings. No proprietary firmware or driver installation is required.
Applications
- Protein purification: Capture and intermediate polishing of IgG, enzymes, and fusion proteins using ion exchange, hydrophobic interaction, or affinity media (e.g., Ni-NTA, Protein A).
- Small-molecule synthesis support: Flash-scale purification of reaction mixtures post-Suzuki coupling, reductive amination, or peptide cyclization.
- Natural product isolation: Gradient elution of plant extracts or microbial fermentation broths on C18, silica, or amino-modified stationary phases.
- Method development: Rapid screening of mobile phase compositions, gradient profiles, and column dimensions prior to transfer to automated HPLC or SFC systems.
- Educational use: Hands-on training in column packing techniques, bed compression optimization, and pressure-flow relationship analysis in university chromatography laboratories.
FAQ
What is the maximum recommended operating pressure for the 6.6 mm ID version?
The 6.6 mm internal diameter EZS-06-25-AF column is rated for continuous operation up to 900 psi (60 bar) at ambient temperature. Short-term excursions to 1000 psi are permissible but not advised for routine use.
Can this column be packed in-house with custom media?
Yes—its axial compression architecture and removable end fittings allow dry or slurry packing with commercially available resins (e.g., Toyopearl, Sepharose, XBridge, Kromasil) using standard slurry delivery manifolds and homogenization protocols.
Is sterilization by autoclaving supported?
No. Borosilicate glass and Chemraz® components are not autoclave-rated. Sterilization must be achieved via 0.22 µm filtration of solvents and sanitization with 70% ethanol or 0.1 M NaOH followed by thorough equilibration.
How does axial compression improve resolution compared to fixed-bed columns?
Axial compression mitigates radial void formation and bed settling during extended runs, preserving linear velocity homogeneity and reducing peak broadening—particularly critical for wide-bore preparative columns operating above 5 mL/min.
Are replacement frits and O-rings available separately?
Yes. PTFE frits (10 µm, 20 µm, 50 µm), Chemraz® O-rings (size-specific kits), and ETFE end caps are stocked as OEM spare parts (catalog numbers available upon request).
