ZR-B02 Airborne Microbial Impinger (AGI) by Junray

Overview

The ZR-B02 Airborne Microbial Impinger (AGI) is a precision-engineered liquid impaction sampler designed for quantitative collection of viable and non-viable airborne microorganisms—including bacteria, fungi, viruses, and bioaerosol particles—from ambient or controlled-environment air. Operating on the principle of inertial impaction via high-velocity jet aspiration, the device draws air through a fine-bore nozzle positioned just above the surface of a sterile collection liquid (e.g., neutralizing buffer, peptone water, or defined growth media). As airflow accelerates through the constricted orifice, particulate-laden air undergoes abrupt directional change; microorganisms—due to their mass-dependent inertia—deviate from the airstream and impact directly into the liquid phase. This physical capture mechanism ensures high collection efficiency (>90% for particles ≥0.5 µm at 12.5 L/min), minimal particle re-aerosolization, and preservation of microbial viability for subsequent culture-based enumeration or molecular analysis (e.g., qPCR, metagenomics).

Key Features

• Optimized nozzle geometry with 0.5 mm internal diameter for consistent jet velocity and reproducible impaction kinetics across standard sampling flow rates (10–15 L/min)
• Chemically resistant borosilicate glass construction (DIN 28120 compliant) ensuring compatibility with disinfectants, organic solvents, and sterilization protocols (autoclaving at 121°C, 20 min)
• Integrated splash guard and baffle design minimizing liquid entrainment and cross-contamination during extended sampling durations (up to 8 hours)
• Modular interface compatible with standardized vacuum manifolds, digital flow controllers, and calibrated rotameters meeting ISO 14644-1 and ASTM D1357 requirements
• Low dead-volume reservoir (15 mL nominal capacity) enabling high-concentration recovery for low-abundance bioaerosols without dilution artifacts
• Traceable manufacturing documentation supporting GLP-compliant environmental monitoring workflows in pharmaceutical cleanrooms and hospital HVAC validation

Sample Compatibility & Compliance

The ZR-B02 AGI is validated for use with microbiological media including Tryptic Soy Broth (TSB), Letheen Broth, and PBS-based neutralizing solutions. Its impaction efficiency profile aligns with ISO 8573-7:2019 Annex C for biological aerosol sampling and supports compliance with USP Environmental Monitoring for Aseptic Processing and EU GMP Annex 1 (2022) requirements for viable particle detection in Grade A/B zones. All units are supplied with individual calibration certificates referencing NIST-traceable flow standards. Device geometry conforms to the AGI-30 specification defined in EPA Method TO-11A for bioaerosol assessment in occupational health surveys.

Software & Data Management

While the ZR-B02 operates as a standalone passive collector, it integrates seamlessly into regulated data ecosystems when paired with Junray’s optional ZR-DataLink module (sold separately). This hardware interface enables real-time logging of cumulative volume, flow rate deviation alerts, and timestamped event markers—exportable in CSV/Excel format with audit trail functionality compliant with FDA 21 CFR Part 11. Raw sampling metadata (including operator ID, location tag, and medium lot number) can be embedded via QR-coded sample vials scanned prior to incubation, facilitating full chain-of-custody tracking in LIMS environments.

Applications

• Pharmaceutical facility environmental monitoring (cleanroom classification per ISO 14644-1, isolator qualification)
• Hospital infection control programs targeting airborne pathogens (e.g., Mycobacterium tuberculosis, Aspergillus spp.)
• Indoor air quality assessments in schools, offices, and public transport systems
• Research-grade bioaerosol characterization in atmospheric science and agricultural epidemiology
• Validation of HVAC filtration efficiency and UVGI system performance per ASHRAE Standard 185.2
• Occupational exposure assessment in wastewater treatment plants and composting facilities

FAQ

What is the recommended sampling flow rate for optimal microbial recovery?
The ZR-B02 achieves peak impaction efficiency between 12.5 L/min and 15 L/min; operation outside this range requires recalibration per ISO 14698-1 Annex B.
Can the impinger be sterilized using ethylene oxide (EtO)?
Yes—borosilicate glass and PTFE components are EtO-compatible; however, post-sterilization aeration per ISO 10993-7 is required to remove residual gas before use.
Is the ZR-B02 suitable for viral aerosol collection?
It has demonstrated efficacy for enveloped and non-enveloped virus capture (e.g., influenza A, MS2 bacteriophage) when used with protein-stabilized collection media and chilled operation (4°C).
Does Junray provide method validation support for regulatory submissions?
Yes—application-specific validation packages (including recovery studies, LOD/LOQ determination, and ruggedness testing per ICH Q2(R2)) are available upon request for FDA, EMA, or PMDA filings.