Yiheng DHP-9272B Electric Constant Temperature Incubator

Overview

The Yiheng DHP-9272B Electric Constant Temperature Incubator is a precision-engineered laboratory incubation system designed for stable, uniform thermal environments in microbiological culture, cell propagation, enzyme assays, and biochemical storage applications. Utilizing six-side direct heating with embedded electric heating films and forced-air convection, the unit ensures rapid thermal response and exceptional spatial temperature homogeneity across its 270 L working chamber. The microprocessor-based PID controller delivers high reproducibility with 0.1 °C resolution and maintains setpoint stability within ±0.5 °C fluctuation and ±0.5 °C uniformity—performance characteristics aligned with ISO 13408-1 (sterile processing) and ASTM E145-22 (standard specification for gravity-convection and forced-convection ovens). Its robust stainless steel mirror-finish interior resists corrosion and facilitates cleaning under GLP-compliant laboratory maintenance protocols.

Key Features

• Microprocessor-controlled digital PID temperature regulation with programmable timer (1–9999 minutes), supporting unattended operation and repeatable experimental cycles.
• Double-layer door design: outer insulated door and inner tempered glass viewing window; automatic suspension of heating and fan circulation upon glass door opening to prevent thermal overshoot and ensure operator safety.
• Six-face heating configuration with high-efficiency electric film elements enables rapid chamber warm-up and minimizes thermal gradients during steady-state operation.
• Intelligent fan speed modulation adjusts airflow intensity based on thermal load and setpoint, reducing evaporative loss from open petri dishes or liquid cultures without compromising uniformity.
• Independent over-temperature safety cut-off system (optional) provides hardware-level protection independent of the main controller—critical for long-duration incubations and regulatory compliance (e.g., FDA 21 CFR Part 11 Annex 11 traceability requirements).
• Standard RS485 interface (optional) supports integration into centralized lab monitoring networks using Modbus RTU protocol; compatible with LIMS and SCADA systems for audit-ready data logging.

Sample Compatibility & Compliance

The DHP-9272B accommodates standard laboratory vessels including Petri dishes (up to 150 mm diameter), multi-well plates (6–96-well), culture flasks (T25–T225), and glass serum bottles. Its chamber dimensions (600 × 600 × 750 mm) allow flexible rack configurations and support stacked shelving (standard: two adjustable stainless steel trays). All materials in contact with the chamber—including interior lining, tray supports, and gasketing—meet USP Class VI biocompatibility standards. The unit complies with IEC 61010-1:2010 for electrical safety in laboratory equipment and conforms to EN 60335-1 for household and similar electrical appliances, ensuring safe deployment in academic, clinical, and industrial QC laboratories.

Software & Data Management

While the base model operates via standalone front-panel controls, optional accessories extend its digital functionality: an embedded thermal printer (model YHP-PRT1) provides real-time hardcopy records of temperature profiles; alternatively, the RS485 interface enables bidirectional communication with third-party software platforms (e.g., LabVIEW, MATLAB, or custom Python-based acquisition tools) for time-stamped CSV export. When configured with the optional intelligent programmable controller, users can define up to 30 segments across 8 ramp/soak cycles—enabling complex protocols such as simulated diurnal temperature shifts or gradual stress acclimation studies. All logged data include timestamp, setpoint, actual chamber temperature, and operational status flags—supporting ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) for GxP-regulated workflows.

Applications

• Bacterial and fungal cultivation (e.g., E. coli, S. aureus, A. niger) per ISO 7218 and CLSI M02-A12 guidelines.
• Mammalian and insect cell line maintenance at 37 °C with CO₂-free ambient air protocols.
• Enzyme activity assays requiring precise thermal control between 25–65 °C (e.g., amylase, protease, lipase kinetics).
• Seed germination testing and plant tissue culture under controlled non-photoperiodic conditions (ASTM D5517).
• Stability testing of pharmaceutical excipients and diagnostic reagents per ICH Q1A(R2) accelerated conditions.
• Quality control incubation for food safety testing (e.g., APC, coliform enumeration per ISO 4833-1).

FAQ

What is the maximum operating temperature of the DHP-9272B?
The unit is rated for continuous operation from ambient +5 °C up to 65 °C.
Does the incubator support humidity control?
No—this is a dry-air constant temperature incubator; humidity control requires a separate humidified model (e.g., Yiheng HPS series).
Can the DHP-9272B be validated for GMP use?
Yes—the design supports IQ/OQ/PQ execution; temperature mapping reports, calibration certificates (NIST-traceable probe), and electronic audit trails (with optional software) fulfill FDA 21 CFR Part 11 and EU Annex 11 expectations.
Is the interior lighting UV-capable?
UV sterilization is available only as an optional add-on (254 nm germicidal lamp); it is not standard equipment.
What power supply configuration is required for installation?
The DHP-9272B operates on single-phase AC 220 V, 50 Hz, with a nominal input power of 3100 W; dedicated 16 A circuit protection is recommended.