JIHEPU SPH-300D Benchtop Single-Shelf Orbital Shaker

Overview

The JIHEPU SPH-300D Benchtop Single-Shelf Orbital Shaker is an engineered solution for precise, reproducible orbital agitation of culture vessels in controlled laboratory environments. Designed for use within externally regulated temperature chambers—such as incubators or dedicated environmental rooms—the SPH-300D operates without integrated heating or cooling elements, distinguishing it from water-bath or refrigerated shakers. Its core mechanism employs a patented single-axis dynamic balance drive system, enabling stable, low-vibration orbital motion at speeds ranging from 50 to 300 rpm with a fixed 50 mm orbit diameter. This configuration ensures uniform mixing and suspension of microbial, mammalian, or yeast cultures in Erlenmeyer flasks (50–1000 mL), test tubes, and microplates—particularly where consistent oxygen transfer, cell suspension, or reagent homogenization is required. The unit’s compact footprint (680 × 615 × 300 mm) and 60 kg mass support stable operation on standard lab benches, while its rigid cold-rolled steel frame—reinforced with structural steel components and finished with corrosion-resistant electrostatic powder coating—ensures mechanical longevity under continuous duty cycles.

Key Features

• Patented single-axis dynamic balance drive system minimizes mechanical noise and vibration, extending bearing life and improving long-term speed stability.
• Eight-segment programmable controller allows independent setting of speed and duration per segment; parameters are retained after power interruption (auto-recovery function).
• High-precision frequency control with ±1 rpm speed accuracy across the full operating range, verified via internal tachometric feedback.
• Soft-start circuitry prevents sudden acceleration, eliminating splash risk and safeguarding sensitive biological samples during initiation.
• Dual-layer safety architecture: independent over-speed audio/visual alarm, leakage current protection, over-current cutoff, and motor thermal shutdown.
• PID-based microprocessor control with intuitive LCD interface supports rapid parameter entry, real-time monitoring, and clock-synchronized scheduling.
• Motor features high-torque, brushless design with sealed bearings—requiring no routine lubrication or maintenance over extended service intervals.
• Pre-programmed memory retains last-used speed; unit ramps gradually to target rpm upon startup, reducing mechanical stress.

Sample Compatibility & Compliance

The SPH-300D accommodates standard laboratory glassware and plastic vessels via interchangeable flask clamps, supporting capacities from 50 mL to 1000 mL (e.g., 24 × 250 mL flasks or 16 × 1000 mL flasks per run). Its orbital motion profile meets typical requirements for aerobic microbial cultivation, protein expression in *E. coli*, and suspension cell passaging. While the device itself does not provide temperature regulation, its operational envelope is validated for ambient temperatures between 10–40°C and relative humidity ≤80% non-condensing—conditions aligned with ISO 15195:2019 (laboratory equipment environmental classification). Electrical safety complies with IEC 61010-1:2010 (Measurement, Control, and Laboratory Equipment – Safety Requirements). Though not certified to FDA 21 CFR Part 11 out-of-the-box, audit-ready data logs (via optional RS-232/USB interface) may be configured to support GLP/GMP-aligned documentation workflows when integrated into validated laboratory systems.

Software & Data Management

The onboard embedded controller provides local parameter management only—no proprietary PC software is bundled. However, the unit includes an RS-232 serial port (optional USB adapter available) enabling integration with third-party laboratory information management systems (LIMS) or custom SCADA platforms. Time-stamped operational logs—including start/stop timestamps, setpoints, actual rpm, and fault events—are accessible via serial query commands. All user-modifiable settings (e.g., program sequences, alarm thresholds) are stored in non-volatile memory with write-protection logic to prevent accidental corruption. For regulated environments, external validation protocols (e.g., IQ/OQ/PQ) can be executed using calibrated tachometers and stopwatch verification against displayed timing functions.

Applications

• Aerobic bacterial and yeast fermentation screening in R&D and pilot-scale bioprocess development.
• Recombinant protein expression in shake-flask cultures under controlled environmental conditions.
• Suspension culture maintenance of hybridoma or CHO cells prior to bioreactor inoculation.
• Chemical reaction mixing where gentle yet consistent agitation is critical to kinetic consistency.
• Media preparation and reagent blending requiring homogeneous dispersion without foaming or shear-induced denaturation.
• Environmental microbiology studies involving soil slurry or water sample homogenization.

FAQ

Is the SPH-300D suitable for use inside a CO₂ incubator?
No. The unit is not rated for humidified, high-CO₂, or elevated-temperature internal environments. It must be operated in a separate, externally temperature-controlled room or chamber.
Does it include temperature control or heating capability?
No. The SPH-300D is a non-thermostatic shaker. Temperature regulation must be provided by the host environment (e.g., incubator, climate-controlled lab).
Can I connect it to a computer for remote monitoring?
Yes—via the optional RS-232 interface (with terminal emulation or custom script), though no vendor-supplied software is included.
What is the maximum flask size supported on the standard shelf?
Up to 1000 mL Erlenmeyer flasks (16 units simultaneously); custom clamp kits accommodate 50–5000 mL vessels.
Is calibration certification provided with the unit?
Factory calibration data (speed and timer verification) is included; full ISO/IEC 17025 traceable calibration requires third-party service.