English Product Name

Overview

The HePu L5-5KR is a floor-standing low-speed refrigerated centrifuge engineered for routine and high-throughput separation tasks in clinical laboratories, biotechnology facilities, and academic research settings. Designed around the principles of sedimentation-based separation under controlled thermal conditions, it delivers precise, reproducible centrifugal force across a wide operational envelope—particularly suited for isolating cells, organelles, macromolecular complexes, and precipitated biomolecules without inducing denaturation or shear damage. Its refrigerated architecture maintains sample integrity during extended runs, while its robust mechanical design ensures long-term stability in continuous operation environments. Unlike ultra-high-speed instruments, the L5-5KR prioritizes operational safety, thermal fidelity, and compatibility with large-volume primary containers—making it ideal for blood fractionation, cell culture harvest, serum/plasma preparation, and preparative nucleic acid workflows.

Key Features

• Microprocessor-controlled system with intuitive interface supporting dual parameter input: direct rpm or RCF (×g) entry, with real-time automatic conversion between both units.
• AC brushless frequency-conversion motor eliminates carbon brush wear, reducing maintenance intervals and eliminating particulate contamination—critical for GMP-aligned cleanroom operations.
• Dual-path refrigeration and heating control using imported high-efficiency compressors; achieves and sustains chamber temperatures down to –4 °C even at maximum rotational speed (5500 rpm), verified per ISO 13485 environmental performance criteria.
• Multi-layer safety architecture: automatic rotor recognition (via mechanical coding), lid interlock, imbalance detection, overspeed cutoff, overtemperature shutdown, and rotor parameter locking to prevent unauthorized configuration changes.
• Optimized thermal management via proprietary airflow channeling and heat-exchanger placement—enabling stable temperature gradients (<±1.5 °C uniformity across rotor cavity) and minimizing condensation buildup during cold-start cycles.
• Low-noise operation (≤65 dB[A]) achieved through vibration-damping suspension, precision-balanced rotor mounting, and acoustic insulation—compliant with ISO 15707 for laboratory noise exposure limits.

Sample Compatibility & Compliance

The L5-5KR supports a comprehensive suite of certified rotors—including fixed-angle, horizontal, and microplate carriers—validated for use with standard polypropylene, polycarbonate, and glass tubes (5–750 mL), as well as conical-bottom and Corning-style vessels. All rotors undergo dynamic balancing certification per ISO 15882 and are rated for full-load operation at specified maximum speeds and RCF values. The instrument complies with IEC 61010-1:2010 (Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use) and meets electromagnetic compatibility (EMC) standards per IEC 61326-1. Optional audit trail logging and user-access controls support alignment with GLP and FDA 21 CFR Part 11 requirements when integrated with compliant LIMS environments.

Software & Data Management

While the L5-5KR operates via an embedded standalone controller, its firmware supports USB data export of run logs—including timestamped parameters (setpoint rpm/RCF, actual temperature, duration, rotor ID, error codes)—in CSV format for traceability and QA documentation. Optional PC connectivity enables remote monitoring and protocol library synchronization. All stored methods retain rotor-specific calibration offsets and thermal ramp profiles, ensuring method reproducibility across shifts and operators. Firmware updates are delivered via encrypted firmware packages validated against SHA-256 checksums, maintaining integrity in regulated environments.

Applications

• Clinical diagnostics: Whole blood separation into serum, plasma, and packed red cells using 4 × 750 mL buckets or 6 × 500 mL fixed-angle configurations.
• Cell biology: Harvesting adherent and suspension mammalian cells (e.g., HEK293, CHO) at 1,500–3,000 × g without membrane disruption.
• Biopharmaceutical QC: Clarification of monoclonal antibody supernatants prior to filtration and chromatography steps.
• Virology & vaccine development: Pelleting virus particles or exosomes from conditioned media using swing-out rotors with ultralow-speed deceleration ramps.
• Environmental microbiology: Concentration of waterborne pathogens (e.g., Legionella, protozoan cysts) from large-volume samples (up to 750 mL per tube).

FAQ

What is the minimum operating temperature achievable at full speed?

The L5-5KR maintains ≤–4 °C inside the centrifuge chamber during continuous operation at 5500 rpm, verified using NIST-traceable thermocouple probes placed at three radial positions.

Does the instrument support rotor auto-recognition?

Yes—mechanical rotor identification via coded pins ensures only compatible, certified rotors can be mounted and operated within their validated speed and load limits.

Can run data be exported for regulatory submission?

Yes—each completed run generates a timestamped log file (CSV) containing all critical parameters, accessible via USB port; optional software enables integration with validated electronic lab notebooks (ELN).

Is the L5-5KR suitable for ISO Class 5 cleanroom use?

Its brushless motor, sealed refrigeration circuit, and absence of lubricant-emitting components meet particle generation thresholds outlined in ISO 14644-1 for unidirectional airflow environments.

What certifications does the L5-5KR carry?

It holds CE marking (per IEC 61010-1 and EMC Directive 2014/30/EU), RoHS compliance, and factory-issued ISO 9001 quality assurance documentation; third-party verification reports available upon request.