Cole-Parmer Chempette Adjustable Single-Channel Manual Pipette
| Brand | Cole-Parmer |
|---|---|
| Origin | Japan |
| Model | 07859-20 |
| Type | Single-Channel Manual Pipette |
| Volume Range | 0.2–10 mL |
| Maximum Capacity | 10 mL |
| Accuracy | ±1% |
| Precision (Repeatability) | ±1% |
| Increment | 0.1 mL |
| Sterilization | Not autoclavable |
| Tip Ejector | Integrated |
| Housing Material | Polypropylene |
| Piston Seal | PTFE-coated |
| Locking Mechanism | Fixed screw for volume setting |
| Compliance | ISO 8655-compliant design principles |
Overview
The Cole-Parmer Chempette Adjustable Single-Channel Manual Pipette (Model 07859-20) is a precision-engineered liquid handling instrument designed for reliable, repeatable volumetric transfer in routine laboratory workflows. Operating on the air displacement principle, this pipette delivers consistent aspiration and dispensing across its full 0.2–10 mL range by maintaining a stable air cushion between the piston and liquid surface. Its mechanical architecture—featuring a corrosion-resistant polypropylene body, PTFE-coated stainless-steel piston, and optimized O-ring placement—minimizes internal friction and thermal drift, supporting long-term metrological stability. Unlike disposable or low-cost alternatives, the Chempette series adheres to fundamental design tenets aligned with ISO 8655–1 through –5 (Pipettes — Part 1: Terms and general requirements; Part 2: Piston-operated volumetric apparatus), ensuring traceable performance under standard ambient conditions (20–25°C, 40–60% RH).
Key Features
- Volume locking mechanism: A dedicated fixed screw secures the set volume, preventing accidental adjustment during high-throughput operations or transport.
- Durable polypropylene construction: Chemically resistant housing withstands exposure to common solvents, acids, and bases encountered in analytical, QC, and preparative labs.
- PTFE-coated piston assembly: Ensures gas-tight sealing within the cylinder bore over extended use cycles while reducing hysteresis and carryover risk.
- Optimized O-ring positioning: Seals are located outside primary wear zones, preserving dimensional integrity and minimizing calibration drift across thousands of actuations.
- Ergonomic tip ejection: Integrated plunger lever enables controlled, low-force tip release—reducing hand fatigue during prolonged pipetting sessions.
- Graduated scale with vernier-style readability: Clear volume markings and fine increment lines (0.1 mL) support rapid, error-resistant setting verification.
Sample Compatibility & Compliance
The Chempette 07859-20 is compatible with standard conical, universal-fit, and low-retention pipette tips (0.2–10 mL nominal capacity). It is not rated for autoclaving; repeated exposure to steam sterilization (>121°C, 15 psi) may compromise piston seal elasticity and housing dimensional stability. While not certified to ISO 8655 with formal test reports, its mechanical layout, material selection, and functional behavior reflect compliance with core metrological requirements—including linearity, accuracy, and repeatability thresholds defined in ISO 8655–2. The device supports GLP- and GMP-aligned workflows when used with documented calibration intervals (recommended annually or per SOP), and its manual operation ensures full auditability without embedded software dependencies.
Software & Data Management
As a fully mechanical, non-electronic pipette, the Chempette 07859-20 requires no firmware, drivers, or connectivity protocols. All operational parameters—including volume setting, actuation force, and tip compatibility—are user-determined and physically verifiable. This analog architecture eliminates cybersecurity concerns, data logging overhead, and validation complexity associated with electronic pipettes. Laboratories implementing 21 CFR Part 11 compliance may document usage via paper-based logbooks or LIMS-integrated manual entry, with calibration certificates maintained separately per instrument ID. No proprietary software or cloud services are involved.
Applications
- Quantitative sample dilution in environmental testing (e.g., EPA Method 525.3, water extract analysis)
- Reagent dispensing for ELISA, PCR setup, and cell culture media preparation
- Standard solution preparation in pharmaceutical QC labs operating under USP Analytical Instrument Qualification guidelines
- Calibration standard transfer in reference material laboratories (ISO/IEC 17025 environments)
- Teaching labs requiring robust, maintenance-free tools for foundational pipetting technique instruction
FAQ
Is the Chempette 07859-20 autoclavable?
No. The polypropylene housing and PTFE piston seal are not rated for steam sterilization. Autoclaving may deform components and degrade sealing performance.
What tip types are compatible with this pipette?
Standard 10 mL conical tips meeting ISO 8655–3 dimensional specifications, including universal-fit and low-binding variants.
Does it require calibration certification upon delivery?
It ships without factory calibration documentation. Users must perform initial verification per internal SOP or ISO/IEC 17025 procedures before first use.
Can it be used with viscous or volatile liquids?
Yes—with appropriate technique adjustments (pre-wetting, reverse pipetting)—though accuracy remains specified only for aqueous solutions at 20°C per ISO 8655–2.
How often should volume verification be performed?
At minimum annually, or after any impact event, cleaning with aggressive solvents, or observed performance deviation—per laboratory quality management system requirements.
