Autosense AF-Series Disposable Syringe Filters
| Brand | Autosense |
|---|---|
| Membrane Materials | Mixed Cellulose Ester (MCE) & Polyvinylidene Fluoride (PVDF) |
| Available Diameters | 13 mm, 25 mm, 50 mm |
| Pore Sizes | 0.18 µm, 0.22 µm, 0.45 µm, 0.8 µm, 1.2 µm |
| Pack Size | 100 units per pack |
| Compliance | ISO 9001-manufactured |
Overview
The Autosense AF-Series Disposable Syringe Filters are precision-engineered membrane filtration devices designed for rapid, reliable, and contamination-free sample clarification prior to analytical instrumentation. Based on the principle of pressure-driven microfiltration, these filters utilize standardized syringe-driven flow to remove particulates, microorganisms, and aggregates from liquid samples—ensuring downstream instrument protection and data integrity. Each unit integrates a chemically resistant membrane supported by a rigid polypropylene housing, with seamless injection-molded construction eliminating adhesive-based delamination risks common in multi-component assemblies. The AF-Series is widely deployed in pharmaceutical QC labs, biotechnology R&D, clinical diagnostics, and environmental testing workflows where reproducible filtration performance and low extractables are non-negotiable.
Key Features
- One-piece injection-molded polypropylene housing ensures structural integrity under typical syringe pressures (up to 6 bar), minimizing risk of housing rupture or membrane collapse.
- Dual membrane chemistry portfolio: hydrophilic Mixed Cellulose Ester (MCE) for aqueous solutions and hydrophobic Polyvinylidene Fluoride (PVDF) for organic solvents—including acetonitrile, methanol, THF, and chloroform—enabling broad compatibility across HPLC, GC, and LC-MS sample preparation protocols.
- Controlled pore size distribution certified per ASTM F838-22 for bacterial retention; 0.22 µm variants provide sterile filtration (≥99.999% removal of Brevundimonas diminuta ATCC 19146), while 0.45 µm filters serve as general particulate removal units compliant with USP <788> subvisible particle testing requirements.
- Low protein binding (<5 ng/cm² for MCE; <10 ng/cm² for PVDF), validated via ELISA and BCA assays—critical for preserving analyte recovery in low-concentration biomolecule applications such as monoclonal antibody characterization and peptide mapping.
- Pre-sterilized options available via gamma irradiation (25–35 kGy), with full sterility assurance documentation (SAL 10⁻⁶) and EO residual testing per ISO 10993-7.
Sample Compatibility & Compliance
The AF-Series supports filtration of diverse sample matrices including cell culture supernatants, serum, plasma, urine, buffer solutions, mobile phases, and organic extracts. All membranes undergo rigorous extractables profiling per USP <661.1> and ISO 10993-12, with certified low leachables (<0.5 µg/mL total organic carbon) ensuring no interference in UV-Vis, fluorescence, or mass spectrometric detection. Units comply with ISO 9001:2015 quality management standards at the manufacturing site in Tianjin, China. Filter housings meet ISO 594-1/2 for Luer-lock interface dimensional tolerances, guaranteeing universal compatibility with standard 1–60 mL plastic or glass syringes. Documentation packages include Certificate of Analysis (CoA), Certificate of Conformance (CoC), and Material Safety Data Sheets (MSDS) upon request—supporting audit readiness for FDA 21 CFR Part 11, EU Annex 11, and ISO/IEC 17025-accredited laboratories.
Software & Data Management
As a passive consumable device, the AF-Series requires no embedded firmware, drivers, or software integration. However, lot-specific traceability is maintained through alphanumeric batch coding laser-etched on each individual filter housing and printed on outer packaging. Batch records—including raw material certifications, membrane lot traceability, sterilization logs (where applicable), and QC test reports—are retained for ≥10 years and accessible upon formal request to support root cause analysis during investigations or regulatory inspections. Integration into electronic lab notebook (ELN) systems or LIMS is achieved via manual entry or barcode scanning using standard GS1-128 labeling conventions.
Applications
- HPLC and UHPLC mobile phase and sample solution filtration to prevent column clogging and extend column lifetime.
- Pre-filtration of biological samples prior to ELISA, Western blotting, or flow cytometry to eliminate cellular debris without altering antigenicity.
- Clarification of fermentation broths and purification intermediates in downstream bioprocessing (e.g., pre-ultrafiltration conditioning).
- Environmental water analysis per EPA Method 160.1 and ISO 5667-12 for suspended solids removal before ICP-MS or AAS quantification.
- Pharmaceutical stability studies requiring particulate-free injectables per USP <788> and Ph. Eur. 2.9.20.
FAQ
Are AF-Series syringe filters suitable for use with aggressive organic solvents such as DMSO or DMF?
Yes—PVDF membrane variants demonstrate excellent chemical resistance to polar aprotic solvents, including DMSO, DMF, and NMP, up to 100% concentration at ambient temperature. Compatibility charts are available upon request.
Do you offer non-pyrogenic or endotoxin-tested versions?
Standard sterile units are tested for endotoxins per USP <85> (LAL assay); endotoxin levels are consistently ≤0.25 EU/mL. Custom endotoxin-free certification (≤0.03 EU/mL) is available under special order.
What is the maximum recommended operating pressure?
Maximum recommended syringe-driven pressure is 6 bar (87 psi) at 23°C. Exceeding this may compromise membrane integrity or housing seal performance.
Can these filters be autoclaved?
No—polypropylene housing and membrane materials are not autoclave-stable. Sterilization is exclusively performed via gamma irradiation or ethylene oxide (EO) gas, depending on configuration.
Is there lot-to-lot performance variability in flow rate or retention efficiency?
No—each production lot undergoes 100% visual inspection and statistical process control (SPC) sampling for bubble point, flow rate at fixed ΔP, and microbial retention. Cpk values ≥1.33 are maintained across all critical parameters.
