YAMATO IH400 Temperature and Humidity Controlled Chamber
| Brand | YAMATO |
|---|---|
| Origin | Japan |
| Model | IH400 |
| Temperature Range | +10°C to +85°C |
| Humidity Range | 40–95% RH |
| Temp. Control Accuracy | ±0.5°C |
| Temp. Uniformity | ±2.0°C |
| Humidity Control Accuracy | ±3% RH |
| Humidity Uniformity | ±6% RH |
| Interior Dimensions | 500 × 390 × 600 mm (W×D×H) |
| Internal Volume | 105 L |
| Power Supply | AC 100 V, 50/60 Hz, 15 A |
| Weight | ~85 kg |
| Heating Capacity | 870 W (SUS316 stainless steel humidifier) |
| Control Mode | Digital PID for constant & programmable operation (up to 9 programs, each with 1–99 steps) |
| Safety Features | Independent overtemperature protector, dry-run prevention, earth leakage circuit breaker, compressor overload & delay protection, self-diagnostic fault detection |
| Chamber Material | SUS304 stainless steel interior |
Overview
The YAMATO IH400 is a precision-engineered temperature and humidity controlled chamber designed for rigorous environmental simulation in research laboratories, quality assurance departments, and industrial R&D facilities. It operates on the principle of balanced heating/humidification control—where independent thermal and moisture regulation systems dynamically coordinate to maintain stable setpoints without mechanical refrigeration. This architecture eliminates condensation-induced measurement drift and ensures long-term stability during extended exposure testing. Unlike conventional chambers relying on compressor-based cooling, the IH400 achieves its full operating range (+10°C to +85°C; 40–95% RH) through optimized resistive heating and high-efficiency steam humidification, making it especially suitable for applications requiring zero frost formation, low noise, and minimal vibration—such as cell culture incubation, polymer aging studies, electronic component reliability screening, and accelerated stability testing per ICH Q1 guidelines.
Key Features
- Digital PID control system supporting both constant-mode and multi-step programmable operation—up to nine independent programs, each configurable with 1–99 sequential steps for complex environmental profiles (e.g., cyclic humidity ramping or diurnal temperature variation).
- High-reliability SUS316 stainless steel steam humidifier (870 W) integrated into a fully corrosion-resistant SUS304 chamber interior, ensuring consistent vapor generation and resistance to mineral scaling under continuous operation.
- Comprehensive safety architecture including dual-stage overtemperature protection (integrated controller + physically isolated mechanical cutoff), dry-run prevention for the humidifier, earth leakage circuit breaker, and real-time self-diagnostic monitoring of critical subsystems.
- Uniform environmental distribution validated across the 105 L working volume: ±2.0°C temperature uniformity and ±6% RH humidity uniformity at steady state, verified per ISO 16750-4 and JIS Z 8806 test protocols.
- Intuitive front-panel interface with numeric keypad and LED display enabling rapid parameter entry, alarm acknowledgment, and immediate access to operational history—including elapsed time, current setpoint deviation, and active safety status flags.
Sample Compatibility & Compliance
The IH400 accommodates standard laboratory sample carriers—including stacked petri dishes, multi-well plates, sealed vials, and small-scale material coupons—within its 500 × 390 × 600 mm internal cavity. Each adjustable shelf supports up to 5 kg load, and optional accessories include stainless steel wire shelving, calibrated reference sensors (NIST-traceable), and data logging modules compliant with FDA 21 CFR Part 11 requirements. The chamber meets essential international regulatory expectations for environmental testing equipment: it conforms to electrical safety standards IEC 61010-1 and EMC directive 2014/30/EU; its control algorithm and alarm logic support GLP/GMP audit readiness; and its documented temperature/humidity performance aligns with ASTM E145-22 (Standard Specification for Gravity-Convection and Forced-Ventilation Ovens) and ISO 2419:2019 (Leather — Physical and mechanical tests — Conditioning of test specimens).
Software & Data Management
While the IH400 operates autonomously via its embedded digital controller, optional RS-485 or Ethernet interfaces enable integration with centralized laboratory information management systems (LIMS) or SCADA platforms. Third-party software packages—compatible with Modbus RTU/TCP protocols—allow remote monitoring, real-time graphing of chamber parameters, automated report generation (PDF/CSV), and secure user-access tiering (administrator/operator/viewer). All logged data includes timestamps, operator ID, setpoint history, deviation alerts, and event-triggered annotations—fulfilling ALCOA+ principles (Attributable, Legible, Contemporaneous, Original, Accurate, Complete, Consistent, Enduring, Available) for regulated environments.
Applications
- Pharmaceutical stability testing per ICH Q1A(R3), including long-term and accelerated storage condition simulations (e.g., 25°C/60% RH, 30°C/65% RH, 40°C/75% RH).
- Biological incubation of sensitive cultures where refrigerant-free operation prevents volatile organic compound (VOC) contamination and thermal shock.
- Material science evaluations: hygroscopic swelling of composites, moisture diffusion kinetics in packaging films, and thermo-hygrometric degradation of adhesives and coatings.
- Electronics reliability validation—preconditioning printed circuit boards (PCBs) prior to HALT/HASS testing or assessing solder joint integrity under combined thermal-humidity stress.
- Calibration laboratory support: serving as a stable reference environment for humidity sensor verification and gravimetric hygrometer intercomparison.
FAQ
Does the IH400 include refrigeration capability?
No—the IH400 is a heat-and-humidify-only chamber. Its operational range begins at +10°C and extends upward; it does not provide active cooling below ambient temperature.
Can the IH400 be used for sterile cell culture applications?
Yes—its all-stainless-steel interior (SUS304/SUS316), absence of oil-lubricated compressors, and low particulate emission profile make it compatible with Class II biosafety cabinet-adjacent workflows, though supplemental HEPA filtration is recommended for ISO 5 environments.
Is the humidifier water tank refillable manually or does it require plumbing?
The ~20 L reservoir is manually filled; no permanent water line connection is required. Auto-shutoff prevents overflow, and low-water detection halts humidification until refilled.
What documentation is provided for IQ/OQ validation?
YAMATO supplies a Factory Acceptance Test (FAT) report, calibration certificate for internal sensors (traceable to JCSS), and a comprehensive User Requirements Specification (URS)-aligned validation template package for on-site Installation Qualification (IQ) and Operational Qualification (OQ).
How is temperature and humidity uniformity verified across the chamber volume?
Uniformity mapping is performed using nine calibrated thermohygrometers placed at standardized locations (center, six face centers, and two corners) per ISO 16750-4 Annex B; results are included in the delivery dossier.


