Yoyilab LRH-150F Biochemical Incubator

Overview

The Yoyilab LRH-150F Biochemical Incubator is a precision-engineered, dual-mode (heating/cooling) environmental chamber designed for stable, long-term cultivation and preservation of microorganisms—including bacteria, yeasts, molds, and fungi—as well as for plant tissue culture, BOD testing, and environmental sample incubation. Built on a robust compressor-based refrigeration system coupled with PID-controlled resistive heating, the unit delivers reliable thermal regulation across its full operational range of 0–65 °C. Its architecture adheres to fundamental principles of convective heat transfer and thermal mass stabilization, ensuring minimal thermal lag and high reproducibility in static incubation protocols. The chamber is optimized for laboratories requiring compliance-ready operation in regulated life science workflows—particularly those aligned with ISO 17025-accredited testing environments, GLP-compliant microbiological assays, and educational or industrial QC applications where temperature fidelity directly impacts colony formation kinetics and metabolic activity.

Key Features

• Microprocessor-based PID temperature control system with 0.1 °C resolution and adaptive tuning—enabling precise setpoint maintenance under variable ambient loads (5–30 °C ambient operating range).
• Dual-zone thermal management: independent compressor cooling and PTC heating elements ensure seamless transition between sub-ambient and elevated temperatures without overshoot or hysteresis.
• Stainless steel interior (mirror-finish 304 grade) with rounded corners and adjustable stainless steel shelves—designed for easy decontamination and compatibility with alcohol-based or hydrogen peroxide vapor (HPV) sterilization cycles.
• Double-glazed, sealed observation door with low-emissivity coating—minimizes radiant heat loss and maintains internal stability during visual monitoring.
• Four-point leveling system with dual-mode mobility: swivel casters (lockable) + adjustable leveling feet—ensures mechanical stability on non-uniform laboratory flooring while supporting safe repositioning.
• 5.7-inch TFT-LCD graphical interface with menu-driven navigation—displays real-time chamber temperature, setpoint, elapsed time, remaining timer duration, and system status flags simultaneously.
• Power-loss recovery function with non-volatile memory: retains all user-defined parameters—including temperature setpoints, timer values, and alarm thresholds—after unexpected shutdowns or grid interruptions.
• Independent over-temperature safety cutoff (hardware-limited, non-software-dependent) compliant with IEC 61000-4-2/4-4 immunity standards for lab-grade electrical safety.

Sample Compatibility & Compliance

The LRH-150F accommodates standard microbiological vessels—including Petri dishes (up to 150 mm), test tubes (16 × 150 mm), Erlenmeyer flasks (up to 2 L), and multi-well plates—on its three adjustable stainless steel shelves (load capacity: 15 kg per shelf, evenly distributed). Its uniformity specification of ±1.5 °C (verified at 25 °C per ISO 15272:2019 Annex B methodology) supports applications requiring adherence to USP /, ASTM D5297 (BOD incubation), and EN ISO 8666 (microbial enumeration). The unit’s R134a-free refrigerant (designated “F” model) complies with EU F-Gas Regulation (EU) No 517/2014 and meets RoHS 3 directive requirements. Optional accessories—including BOD-rated power outlets, RS485 Modbus RTU interface (with traceable calibration log export), and 25 mm/50 mm pass-through ports—facilitate integration into validated laboratory information management systems (LIMS) and 21 CFR Part 11–compliant data workflows.

Software & Data Management

While the LRH-150F operates autonomously via its embedded controller, optional RS485 communication enables bidirectional data exchange with external SCADA or LIMS platforms using standard Modbus RTU protocol. Time-stamped temperature logs (including min/max/mean per 1-minute interval) can be exported in CSV format via connected PC software—supporting audit trails required under GLP (OECD Series No. 1) and GMP Annex 11. An optional embedded thermal printer provides hard-copy records of session start/end times, deviation alerts, and calibration verification events—fully traceable to NIST-traceable reference thermistors installed at three spatially distributed points (top/mid/bottom). All firmware updates are delivered via secure USB key; no cloud dependency or internet connectivity is required for core functionality.

Applications

• Biochemical oxygen demand (BOD₅) testing per APHA Standard Methods 5210B and ISO 5815-1.
• Microbial culture and viability assessment in pharmaceutical QC labs (e.g., sterility testing support, media growth promotion checks).
• Environmental monitoring programs—water, soil, and air sample incubation per EPA Method 1681 and ISO 9308-1.
• Plant physiology studies including seed germination, callus induction, and photoperiod-independent growth trials.
• Veterinary diagnostics and aquaculture pathogen isolation (e.g., Vibrio, Aeromonas spp.) under controlled thermal stress conditions.
• Academic teaching labs requiring durable, serviceable incubators capable of sustaining >10,000 hours of cumulative runtime with scheduled preventive maintenance.

FAQ

What is the maximum ambient temperature at which the LRH-150F maintains 0 °C internally?
The unit is rated for continuous operation at ambient temperatures up to 30 °C. At 30 °C ambient and 50% RH, it achieves and sustains 0 °C with ≤1.0 °C fluctuation, per factory validation reports.
Is the LRH-150F suitable for CO₂-sensitive cell cultures?
No—it is a non-CO₂, convection-only incubator. For mammalian cell culture requiring pH control, a dedicated CO₂ incubator with humidification and gas blending is required.
Can the unit be validated for IQ/OQ/PQ according to GxP guidelines?
Yes. Documentation packages—including DQ templates, FAT/SAT checklists, and sensor calibration certificates (NIST-traceable)—are available upon request for qualified customers.
Does the “F” suffix indicate compliance with any specific environmental regulations?
Yes. The “F” denotes use of R600a (isobutane) refrigerant, fully compliant with EU F-Gas Regulation phase-down schedules and exempt from global warming potential (GWP) taxation under national climate policies.
What is the expected service life of the compressor under continuous 24/7 operation?
Based on accelerated life testing per ISO 13374-2, the hermetic rotary compressor is rated for ≥35,000 hours MTBF when operated within specified ambient and load conditions.