AB Sciex QTRAP 2000 LC/MS/MS System with NanoSpray Source and Nano-HPLC Integration

Overview

The AB Sciex QTRAP 2000 LC/MS/MS is a hybrid triple quadrupole/linear ion trap mass spectrometer engineered for quantitative and qualitative analysis in regulated and research laboratory environments. Combining the sensitivity and dynamic range of a triple quadrupole with the structural elucidation capabilities of a linear ion trap, the QTRAP platform enables both targeted quantitation (via MRM mode) and information-dependent acquisition (IDA) for compound identification — all within a single analytical run. This refurbished system includes factory-aligned NanoSpray™ ionization source with integrated video monitoring for real-time capillary alignment verification, and is fully coupled to an LC Packings Nano-HPLC system (model UltiMate 3000 RSLCnano), enabling robust nanoflow LC separation at flow rates from 50 nL/min to 10 µL/min. The instrument was originally manufactured in Canada under SCIEX’s ISO 9001-certified production standards and remains compliant with core performance criteria defined in SCIEX Service Bulletin SB-2000-08.

Key Features

• Hybrid QqLIT architecture: Triple quadrupole front end for high-sensitivity MRM quantification; linear ion trap rear section for MSⁿ fragmentation and rapid scanning
• NanoSpray™ ion source with Peltier-cooled desolvation gas, adjustable spray voltage (0–2.5 kV), and integrated high-resolution video camera for visual alignment and maintenance documentation
• Full integration with LC Packings UltiMate 3000 RSLCnano system: Includes nano-flow pump, autosampler with low-volume injection loop (1–100 nL), column oven, and switching valves for 2D-LC compatibility
• Mass range: m/z 10–2000; unit mass resolution in both Q1 and Q3; scan speed up to 6,000 Da/sec in survey scan mode
• Refurbishment includes full detector gain calibration, vacuum system leak check (<5×10⁻⁸ Torr base pressure), ion optic cleaning and alignment, and firmware update to version 1.4.2
• Complies with SCIEX-recommended preventive maintenance intervals and retains full compatibility with Analyst 1.6.3 software

Sample Compatibility & Compliance

The QTRAP 2000 supports broad sample classes including peptides, metabolites, lipids, small-molecule pharmaceuticals, and environmental contaminants. Its nanoflow configuration enhances sensitivity for low-abundance analytes in proteomics and biomarker discovery workflows. All refurbished units undergo traceable functional verification per ASTM E2623-20 (Standard Practice for Verification of Mass Spectrometer Performance). System documentation includes as-found/as-left test reports, vacuum integrity logs, and ion transmission efficiency validation across five reference compounds (reserpine, MRFA, Glu-Fib, Leu-Enkephalin, and Bradykinin). The instrument meets baseline requirements for GLP-compliant laboratories when operated with audit-trail-enabled software configurations and controlled access protocols.

Software & Data Management

Operates exclusively with SCIEX Analyst® 1.6.3 software (licensed, not included with hardware), supporting method development, acquisition control, peak integration, and report generation. Raw data files (.wiff) are fully compatible with third-party processing tools including PeakView®, MasterView™, and open-source platforms such as Skyline and ProteoWizard. Audit trail functionality is available when deployed on Windows Server environments with domain authentication and file-system-level write protection. Data archiving follows NIST SP 800-53 Rev. 4 guidelines for integrity preservation, and raw datasets retain embedded instrument metadata (calibration dates, operator ID, sequence logs) required for FDA 21 CFR Part 11 compliance when paired with appropriate electronic signature controls.

Applications

• Targeted quantitative proteomics (e.g., SRM/MRM assays for post-translational modifications)
• Metabolite identification and reaction phenotyping in drug development (CYP450 inhibition/induction studies)
• Residue analysis in food safety testing (veterinary drugs, mycotoxins, pesticides at sub-ppb levels)
• Environmental monitoring of emerging contaminants (PFAS, pharmaceuticals in wastewater)
• Method transfer and backup instrumentation in QC labs operating under ISO/IEC 17025 accreditation

FAQ

Is this system eligible for extended service contracts beyond the 6-month warranty?
Yes — eligible customers may purchase SCIEX-certified Extended Service Agreements (ESAs) directly through authorized SCIEX service partners, subject to pre-shipment hardware assessment.
Does the refurbishment include replacement of critical consumables?
Yes — all ion guides, detector dynodes, turbo pump bearings, and ESI/NanoSpray capillaries are replaced with genuine SCIEX parts during refurbishment.
Can this system be upgraded to support newer software versions?
Analyst 1.7.x is supported with optional hardware controller firmware update; Analyst 2.x requires QTRAP 4000 or later and is not compatible.
What documentation accompanies the system?
Shipment includes Certificate of Conformance, Functional Test Report, Vacuum Integrity Log, Calibration Summary, and complete service history extracted from SCIEX’s global instrument database.
Is remote diagnostic support available post-installation?
Yes — SpectraLab Scientific provides 30 days of complimentary remote technical assistance for installation validation and basic method transfer support.